公开(公告)号：WO1997024621A2

公开(公告)日：1997-07-10

申请号：PCT/US1996019544

申请日：1996-12-16

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  ADAMCZYK, Maciej ,  FISHPAUGH, Jeffrey, R. ,  HEUSER, Kevin ,  RAMP, John, M. ,  WONG, Martin

IPC: G01N33/72

CPC classification number: C07D207/337 ,  C07D405/12 ,  G01N33/532 ,  G01N33/72 ,  G01N33/84

Abstract: Immunoassay methods and reagents for measuring the levels of lead in a test sample via the quantification of porphobilinogen use antibodies prepared with compounds of Formulas (II) and (III), wherein X and Y are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to an immunogenic carrier material P wherein P is preferably bovine serum albumin. Also described are the synthesis of fluorescein tracers of the structure of Formulas (V) and (VII), wherein A and C are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to a detectable moiety Q, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.

Abstract translation: 用于通过定量用式（II）和（III）化合物制备的胆色素原使用抗体来测定测试样品中铅含量的免疫测定方法和试剂，其中X和Y是由0至2个杂原子组成的连接基团和0至 8个碳原子与免疫原性载体材料P连接，其中P优选为牛血清白蛋白。 还描述了式（V）和（VII）结构的荧光素示踪剂的合成，其中A和C是连接到可检测部分Q的0至2个杂原子和0至8个碳原子组成的连接基团，其中Q 是可检测的部分，优选荧光素或荧光素衍生物。

公开(公告)号：EP0876616A2

公开(公告)日：1998-11-11

申请号：EP96943648.0

申请日：1996-12-16

Applicant: Abbott Laboratories

Inventor： ADAMCZYK, Maciej ,  FISHPAUGH, Jeffrey, R. ,  HEUSER, Kevin ,  RAMP, John, M. ,  WONG, Martin

IPC: C07D207 ,  C07D405 ,  C07K16 ,  G01N33

CPC classification number: C07D207/337 ,  C07D405/12 ,  G01N33/532 ,  G01N33/72 ,  G01N33/84

Abstract: Immunoassay methods and reagents for measuring the levels of lead in a test sample via the quantification of porphobilinogen use antibodies prepared with compounds of Formulas (II) and (III), wherein X and Y are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to an immunogenic carrier material P wherein P is preferably bovine serum albumin. Also described are the synthesis of fluorescein tracers of the structure of Formulas (V) and (VII), wherein A and C are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to a detectable moiety Q, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.