公开(公告)号：JPH09140380A

公开(公告)日：1997-06-03

申请号：JP30138895

申请日：1995-11-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： BABA TERUHIKO ,  NAMIKAWA HIROYUKI ,  HADO MASAKATSU ,  MOTOKI AKIHIRO ,  HIRANO MASAHIKO

IPC: G01N33/545 ,  C07K17/02 ,  C12N11/08

Abstract: PROBLEM TO BE SOLVED: To provide the subject substrate capable of immobilizing a membrane protein useful in a bioreactor engineering field, etc., to enable activity control of the protein by blending a specific glucolipid with an anionic lipid as components. SOLUTION: A synthetic glucolipid represented by the formula [R is an 12-18C straight-chain saturated alkyl or a straight-chain unsaturated alkyl or phytanyl; (n) is an integer of 1-7] is mixed with an anionic lipid (e.g. sulfoquinobosyl diglyceride) as components to prepare a lipid membrane substrate for immobilizing membrane protein and the protein is dissolved in chloroform into 10mol% concentration and the solvent is distilled away from the solution to prepare the phospholipid thin film and subjected to vacuum drying one night and a buffer aqueous solution is added thereto to prepare a multilamellar vesicle and filtered using a filter having 0.1μm pores under nitrogen gas pressure to afford a single membrane vesicle and the membrane protein is immobilized to provide the objective lipid membrane.

公开(公告)号：JP2000344797A

公开(公告)日：2000-12-12

申请号：JP15264199

申请日：1999-05-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： BABA TERUHIKO ,  NAMIKAWA HIROYUKI ,  HADO MASAKATSU ,  MOTOKI AKIHIRO ,  HIRANO MASAHIKO

IPC: B01F17/42 ,  B01F17/56 ,  C07K14/405 ,  C07K14/415

Abstract: PROBLEM TO BE SOLVED: To provide a method for industrially advantageously producing a lipid vesicle membrane incorporated with a photosynthetic membrane protein, having no restriction on concentration of a surfactant used, capable of increasing the incorporation efficiency of the protein in spite of simple treatment operation and highly maintaining the water-splitting activity ability of photochemical system II protein aggregate. SOLUTION: In this method for producing a lipid vesicle membrane incorporated with a photosynthetic membrane protein by incorporating a photosynthetic membrane protein into a lipid vesicle membrane in the presence of a surfactant, an osmotic substance such as preferably glycinebetaine is further added to the production. Moreover, the osmotic substance is glycine betaine and a photosynthetic membrane protein is a photochemical system II protein aggregate. A lipid constituting a vesicle membrane is not particularly restricted if the lipid is in a liquid crystal state in a dialysis temperature condition. A natural phospholipid, natural glycolipid, synthetic phospholipid, synthetic glycolipid, a synthetic amphiphatic substance having vesicle membrane forming properties, etc., may be cited as the lipid.

公开(公告)号：JPH11123096A

公开(公告)日：1999-05-11

申请号：JP18872998

申请日：1998-07-03

Applicant: AGENCY IND SCIENCE TECHN

Inventor： BABA TERUHIKO ,  NAMIKAWA HIROYUKI ,  HADO MASAKATSU ,  MOTOKI AKIHIRO ,  HIRANO MASAHIKO

IPC: C12P3/00 ,  A61K9/127 ,  C07K14/405 ,  C07K14/415 ,  C08L5/00 ,  C08L89/00

Abstract: PROBLEM TO BE SOLVED: To obtain a photosynthetic membrane protein-integrated vesicle membrane, which does not cause any low-temperature trouble, can be used at a wide range of temperature, retains proton and the like within the vesicle in a closed state for a long time, has stable tolerance to electric potential difference generated by proton gradient and the like, and is useful to utilize ion gradient separated by a membrane, wherein a lipid layer is a vesicle membrane. SOLUTION: The photosynthetic membrane protein-integrated vesicle membrane, which a photosynthetic membrane protein is integrated in a vesicle membrane comprising a 1,3-bisphytanylglycerylether-type oligosaccharide glycolipid and an anionic lipid, is obtained by adding a surfactant to a vesicle membrane comprising a 1,3-bisphytanylglycerylether-type oligosaccharide glycolipid and an anionic lipid at such a concentration of the surfactant that the vesicle membrane is not solubilized even partially but only swollen, then adding a photosynthetic membrane protein, followed by removal of the surfactant.

公开(公告)号：JPH10306062A

公开(公告)日：1998-11-17

申请号：JP11698797

申请日：1997-05-07

Applicant: AGENCY IND SCIENCE TECHN

Inventor： NAKAWA YOSHINOBU ,  BABA TERUHIKO ,  KINUGASA SHINICHI ,  NAKANISHI HIROSHI

IPC: G01N33/544 ,  C07C68/02 ,  C07C69/96 ,  C07J7/00

Abstract: PROBLEM TO BE SOLVED: To provide a novel sterol derivative that is useful for producing vesicles of good stability. SOLUTION: This compound is a sterol derivative represented by the formula (n is 4-8; R and R' are each a sterol having OH in the 3β-position), typically α,ω-bis(cholesteryloxycarbonly)hexaethylene-glycol. The sterol derivative of the formula is prepared by reaction of an oligoethylene glycol and a sterol halognoformate. When vesicles are produced, the sterol derivative of the formula is mixed with a cation, or with a phospholipid, or with a long-chain aliphatic amine or with a surfactant. The vesicles prepared from this cholesterol derivative can include a variety of hydrophilic substances.