公开(公告)号：JPH10182453A

公开(公告)日：1998-07-07

申请号：JP1221698

申请日：1998-01-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KONO YASUHIRO ,  HIGASHIHARA TAKANORI ,  NAKANISHI HIROSHI ,  NAKAWA YOSHINOBU ,  OKA SHUICHI ,  ASADA SHINKO ,  INOUE MASAMI ,  NAKAGOME KAZUYA

IPC: C12P17/14 ,  A01N43/26 ,  A61K31/39 ,  A61P31/04 ,  A61P37/08 ,  C07D327/04 ,  C12N1/20 ,  C12R1/01

Abstract: PROBLEM TO BE SOLVED: To obtain an antiallergic agent having antimicrobial activities against fish diseases and antimicroalgal and antiallergic activities by make the agent include thiotropocin as an active ingredient. SOLUTION: This antiallergic agent comprises thiotropocin, separated and collected from a cultured product prepared by culturing, e.g. a microorganism belonging to the genus Caulobacter (FERM BP-5080) in a culture medium under conditions of a pH within the range of 7.4-7.6, 10-35 deg.C temperature, 5-7 days, aeration spinner culture, etc., as an active ingredient. The method for administering the objective antiallergic agent is carried out by including 5-10% thiotoropocin in, e.g. lactose which is a formulation substrate, providing a powdery or a granular form, mixing the prepared formulation in a daily dose of 0.1-0.5g/kg body weight of fishes with a formula feed and administering the resultant formula feed to the fishes for 5-7 days.

公开(公告)号：JPH10306062A

公开(公告)日：1998-11-17

申请号：JP11698797

申请日：1997-05-07

Applicant: AGENCY IND SCIENCE TECHN

Inventor： NAKAWA YOSHINOBU ,  BABA TERUHIKO ,  KINUGASA SHINICHI ,  NAKANISHI HIROSHI

IPC: G01N33/544 ,  C07C68/02 ,  C07C69/96 ,  C07J7/00

Abstract: PROBLEM TO BE SOLVED: To provide a novel sterol derivative that is useful for producing vesicles of good stability. SOLUTION: This compound is a sterol derivative represented by the formula (n is 4-8; R and R' are each a sterol having OH in the 3β-position), typically α,ω-bis(cholesteryloxycarbonly)hexaethylene-glycol. The sterol derivative of the formula is prepared by reaction of an oligoethylene glycol and a sterol halognoformate. When vesicles are produced, the sterol derivative of the formula is mixed with a cation, or with a phospholipid, or with a long-chain aliphatic amine or with a surfactant. The vesicles prepared from this cholesterol derivative can include a variety of hydrophilic substances.

公开(公告)号：JPH10182314A

公开(公告)日：1998-07-07

申请号：JP1221498

申请日：1998-01-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KONO YASUHIRO ,  HIGASHIHARA TAKANORI ,  NAKANISHI HIROSHI ,  NAKAWA YOSHINOBU ,  OKA SHUICHI ,  ASADA SHINKO ,  INOUE MASAMI ,  NAKAGOME KAZUYA

IPC: C12P17/00 ,  A01N43/22 ,  A01N43/26 ,  A01N63/02 ,  A61K31/38 ,  A61K31/39 ,  A61P31/04 ,  A61P37/08 ,  C07D327/04 ,  C12R1/01

Abstract: PROBLEM TO BE SOLVED: To obtain an antimocroalgal agent excellent in new kinds of bioactivity such as antimicrobial activity for fish diseases, antimicroalgal activity and antiallergic activity by using a specific seven-membered heterocyclic compound bearing a sulfur atom as the active ingredient. SOLUTION: This antimicroalgal agent uses thiotropocin obtained by use of a microorganism belonging to the genus Caulobacter and having the ability to produce thiotropocin as the active ingredient. The microorganism is represented by the strain of Caulobacter sp. PK-654 (National Institute of Bioscience and Human-Technology, reference No. FERM BP-5080). The concentration of the active ingredient is suitably 0.01-10wt.% in emulsions, wettable powders or oils and 0.1-10wt.% in granule or dust.

公开(公告)号：JPH0919294A

公开(公告)日：1997-01-21

申请号：JP19118395

申请日：1995-07-04

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KONO YASUHIRO ,  HIGASHIHARA TAKANORI ,  NAKANISHI HIROSHI ,  NAKAWA YOSHINOBU ,  OKA SHUICHI ,  ASADA SHINKO ,  INOUE MASAMI ,  NAKAGOME KAZUYA

IPC: C12P17/00 ,  A01N43/26 ,  A61K31/38 ,  A61K31/39 ,  A61P37/08 ,  C07D327/04 ,  C07D337/02 ,  C12N1/20 ,  C12P17/14 ,  C12R1/01

Abstract: PROBLEM TO BE SOLVED: To efficiently obtain thiotropocin useful as e.g. an antimicrobial agent for fish diseases, anti-fine algal agent, antiallergic agent, by culturing in a medium microbes belonging to Caulobacter and having ability to produce thiotropocin and by gathering the resultant product from the cultured product. SOLUTION: Microbes belonging to Caulobacter and capable of producing thiotropocin [e.g. Caulobacter sp. PK 654 strain (FERM BP-5080)] are inoculated into a medium followed by conducting a shaking culture at 20 deg.C for 5 days at 45rpm to obtain a seed culture fluid which is then inoculated into a medium- contg. triangular flask followed by conducting a culture at 20 deg.C for 5-7 days, and the resultant culture fluid is centrifuged to gather the resultant supernatant which is then filtered and subjected to column chromatography, and the resultant active fraction is recrystallized from methanol, thus efficiently affording the objective thiotropocin useful as an active ingredient of e.g. antimicrobial agents for fish diseases, anti-fine algal agents, antiallergic agents.

公开(公告)号：JPS6475466A

公开(公告)日：1989-03-22

申请号：JP23139187

申请日：1987-09-16

Applicant: AGENCY IND SCIENCE TECHN

Inventor： NAKAWA YOSHINOBU ,  HONDA KOICHI ,  NAKANISHI HIROSHI

IPC: C07D209/60 ,  C07D403/04 ,  C07D403/10 ,  C07D487/04

Abstract: NEW MATERIAL:An indole derivative shown by formula I (Rn is the same or different and H, alkyl, halogen, amino, amido, nitro, group shown by formula II or group shown by formula III subjected to ortho-condensation to naphthalene ring; Wn and Zn are the same or different and H, halogen, alkyl, -COOH or ester thereof; n is 1 or 2). EXAMPLE:Diethyl 9-( 4, 5-diethoxycarbonyl-1H-1,2,3-triazol-1-yl)-1H-benz(g)in-dole-2, 3-dicarboxylate. USE:Useful as a drug, agricultural chemical and industrial chemical and also useful as a synthetic intermediate thereof. PREPARATION:A triazolylnaphthalene derivative shown by formula IV (Rn' is H, halogen or alkyl) as a raw material is irradiated with light rays to give a compound shown b formula I.

公开(公告)号：JPH10182454A

公开(公告)日：1998-07-07

申请号：JP1221398

申请日：1998-01-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： KONO YASUHIRO ,  HIGASHIHARA TAKANORI ,  NAKANISHI HIROSHI ,  NAKAWA YOSHINOBU ,  OKA SHUICHI ,  ASADA SHINKO ,  INOUE MASAMI ,  NAKAGOME KAZUYA

IPC: C12P17/14 ,  A01N43/26 ,  A61K31/39 ,  A61P31/04 ,  A61P33/00 ,  A61P37/08 ,  C07D327/04 ,  C12N1/20 ,  C12R1/01

Abstract: PROBLEM TO BE SOLVED: To obtain an antimicrobial agent for fish diseases having antimicrobial activities against the fish disease and antimicroalgal and antiallergic activities by making the agent include thiogropocin as an active ingredient. SOLUTION: This antimicrobial agent for fish diseases comprises thiotropocin, separated and collected from cultured product prepared by culturing, e.g. a microorganism belonging to the genus Caulobacter (FERM BP-5080) under conditions of a pH within the range of 7.4-7.6, 10-35 deg.C temperature, 5-7 days, aeration spinner culture, etc., as an active ingredient. The method for administering the objective antimicrobial agent for the fish diseases is carried out by including 5-10% thiotorpicin in, e.g. lactose, which is a preparation substrate, providing a powdery or a granular form, mixing the preparation in a daily dose of 0.1-0.5g/kg body weight of the fishes with a blended feed and administering the resultant blended feed to the fishes for 5-7 days.