公开(公告)号：JPH02100681A

公开(公告)日：1990-04-12

申请号：JP25226588

申请日：1988-10-06

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IZUKURA MASAHIRO ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  C07K1/16 ,  C07K1/22 ,  C07K14/00 ,  C07K14/575 ,  C07K14/655 ,  C07K14/675 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N15/12 ,  C12N15/62 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a recombinant plasmid pENDB 15 capable of producing beta-endorphin stably replicated in Escherichia coli and having strong analgesic activity and having DNA sequence of 4718 base pair. CONSTITUTION:The novel recombinant plasmid pENDB15 has size of 4718 base pair and provides trimethoprim resistance and ampicillin resistance to Escherichia coli which is a host. The above-mentioned plasmid is obtained by replacing sequence of 26 base pair containing sequence coding MEK between BamHI site of pMEK2 (patent application No.63-79680) and XhoI site with chemically synthesized DNA of 104 base pair containing sequence coding beta- endorphin. Escherichia coli transformed by the pENDB15 is deposited as FERM BP-2029 to Fermentation Research Institute.