公开(公告)号：US3904587A

公开(公告)日：1975-09-09

申请号：US30107772

申请日：1972-10-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： TANAKA YOSHIO ,  KATO MASAO ,  OKADA AKIRA ,  SHIMURA YUKIO

IPC: C08F2/00 ,  C08F2/04 ,  C08F20/00 ,  C08F20/32 ,  C08F26/00 ,  C08F220/32 ,  C08F246/00 ,  C09D201/02 ,  C08G22/04 ,  C08F1/08 ,  C08F17/00

CPC classification number: C08F220/32

Abstract: A polymer capable of self-curing or cross-linking at room temperature is produced by free radical polymerization in a nonprotonic solvent of: A. i. a combination of (a) at least one ethylenically unsaturated compound having an epoxy group and (b) at least one ethylenically unsaturated compound having a tertiary amino group selected from the group consisting of an N,Ndisubstituted amino group, a pyridyl group, a quinolyl group and an isoquinolyl group or II. AT LEAST ONE ETHYLENICALLY UNSATURATED COMPOUND HAVING ONE EPOXY GROUP AND ONE TERTIARY AMINO GROUP SELECTED FROM THE GROUP CONSISTING OF AN N,N-disubstituted amino group, a pyridyl group, a quinolyl group and an isoquinolyl group, and optionally B. at least one free radical-copolymerizable ethylenically unsaturated compound other than the ethylenically unsaturated compounds (i) and (ii) and which is free of active hydrogen atoms.

公开(公告)号：DE2252866A1

公开(公告)日：1973-05-10

申请号：DE2252866

申请日：1972-10-27

Applicant: AGENCY IND SCIENCE TECHN

Inventor： TANAKA YOSHIO ,  KATO MASAO ,  MINOWA MICHIKO ,  OKADA AKIRA ,  SHIMURA YUKIO

IPC: C08F2/04 ,  C08F220/32 ,  C08G33/14

公开(公告)号：JPH0638786A

公开(公告)日：1994-02-15

申请号：JP21813091

申请日：1991-05-21

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  FURUSAWA KIYOTAKA ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/53 ,  C12N15/62 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To provide a new manifestation vector useful for the production of a protein containing a heterogenic product fused to the carboxy-terminal of DHFR. CONSTITUTION:The manifestation vector pTP104-4 having the DNA sequence of formula. The vector can be prepared by introducing the terminator region of rrnB gene to the downstream of the DHFR gene of pTP70-1.

公开(公告)号：JPH04351607A

公开(公告)日：1992-12-07

申请号：JP12333591

申请日：1991-05-28

Applicant: SANKYO CO ,  AGENCY IND SCIENCE TECHN

Inventor： KANEKO MASAKATSU ,  KAMOGARI MAKOTO ,  KOBAYASHI TOMOO ,  HIRANO TAKASHI ,  OHASHI SHINICHI ,  TANAKA YOSHIO

IPC: A61K31/70 ,  A61K31/7042 ,  A61K31/7052 ,  A61K31/7064 ,  A61K31/7072 ,  A61K47/48 ,  A61P35/00 ,  C07H19/067 ,  C08F8/12 ,  C08F8/30 ,  C08F16/32 ,  C08F216/12 ,  C08F220/04 ,  C08F222/06

Abstract: PURPOSE:To obtain a polymer complex having good sustained release properties and strong anti-tumor activity against tumor cells by reacting a divinyl ether- maleic anhydride copolymer with a 5-fluorouridine derivative. CONSTITUTION:The title complex is obtained by reacting a divinyl ether-maleic anhydride copolymer represented by formula I (wherein n is 10 to 30) with a compound represented by formula II (wherein m is 1 to 19) and hydrolyzing the acid anhydride moieties remaining unreacted. It has a structural unit selected from those represented by formulae III to V, and has a -CONH-/-COO- ratio of 0.01-1.0. Preferred examples of the complex include one obtained from a copolymer of formula I (wherein n is an integer of 18 to 23), one having a -CONH-/-COO- ratio of 0.1-0.4, one obtained using a compound of formula II (wherein m is an integer of 2 to 15), one having an average molecular weight of 6,000-26,000, and one containing a structural unit represented by formula IV.

公开(公告)号：JPH04117286A

公开(公告)日：1992-04-17

申请号：JP12320390

申请日：1990-05-15

Applicant: AGENCY IND SCIENCE TECHN ,  HITACHI CHEMICAL CO LTD

Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  TANAKA YOSHIO ,  BABA KENZO ,  IZUTSU HIROSHI ,  OBARA KAZUHIKO ,  TAKASUKA AKIKO

IPC: C12N15/09 ,  A61K31/00 ,  A61K38/00 ,  A61K38/44 ,  A61P37/08 ,  C07K1/12 ,  C07K1/22 ,  C07K7/06 ,  C07K14/00 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12N15/12 ,  C12N15/53 ,  C12N15/62 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

Abstract: NEW MATERIAL:A recombinant plasmid containing a base sequence encoding dihydrofolate reductase-antiallergic peptide polymer fused protein (II). EXAMPLE:A recombinant plasmid wherein an antiallergic pentapeptide, a fused protein (II) of dimer contains a base sequence encoding an amino acid sequence shown by the [formula (underline shows amino acids existing between dihydrofolate reductase and antiallergic pentapeptide). USE:Production of DSDGK. PREPARATION:A DNA encoding DSDGK polymer obtained by chemically synthesizing a single strand DNA and annealing both chains is inserted into a vector plasmid capable of manifesting DHFR polymer.

公开(公告)号：JPH02138981A

公开(公告)日：1990-05-28

申请号：JP29338988

申请日：1988-11-19

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  TANAKA YOSHIO

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/575 ,  C07K14/60 ,  C07K19/00 ,  C12N1/21 ,  C12N15/18 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To mass-produce a GRF1-29 derivative by forming a recombinant plasmid pSG1-12 having a DNA sequence coding a peptide fragment of GRF with a fused protein of dihydrofolate reductase. CONSTITUTION:A recombinant plasmid pSG1-12, stably replicative in Escherichia coli, capable of imparting tolerance to trimethoprim and ampicillin to Escherichia coli which is a host, coding a fused protein of a peptide fragment of a growth hormone releasing factor having a specific amino acid sequence and a dihydrofolate reductase and forming a recombinant plasmid and having a specific DNA sequence is formed. The recombinant plasmid encodes DHFR- GRFM and Escherichia coli having the pSG1-12 is capable of accumulating and producing the DHFR-GRFM in a large amount.

公开(公告)号：JPH01252290A

公开(公告)日：1989-10-06

申请号：JP7968188

申请日：1988-03-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  KOKUBU TOMOKUNI ,  FURUSAWA KIYOTAKA ,  OHASHI SHINICHI ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  A61K38/22 ,  A61P23/00 ,  A61P25/04 ,  C07K1/14 ,  C07K1/22 ,  C07K14/00 ,  C07K14/575 ,  C07K14/655 ,  C07K14/70 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a large amount of dihydrofolic acid reductaseleucine enkephalin fused protein, by manifesting a fused gene in which a leucine enkephalin gene is included in a plasmid vector pTP 104-4 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pLEK 1, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4207 basic pairs and having a bonded structure of chemically synthesized DNA of 34 basic pairs containing conformations coding BamHI of pTP 104-4, 4173 basic pairs DNA fragments of large side obtained by Sa I abscission and a leucine enkephalin, is produced. A colon bacillus introduced of the plasmid pLEK 1 (FERM BP-1818) produces a large amount of dihydrofolic acid reductase-leucine enkephalin fused protein.

公开(公告)号：JPH01252287A

公开(公告)日：1989-10-06

申请号：JP7967888

申请日：1988-03-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  C07K1/14 ,  C07K1/22 ,  C07K7/14 ,  C07K14/00 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a large amount of dihydrofolic acid reductase-angiotensin fused protein, by manifesting a fused gene in which an angiotensin I gene is included in a recombined plasmid pLEK1 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pANG1-23, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4222 basic pairs and having a replaced structure with a conformation of 28 basic pairs containing a conformation coding leucine enkephaline between BamHI site and MluI site in pLEK1 to a chemically synthesized DNA of 43 basic pairs containing a conformation coding angiotensin I, is produced. A colon bacillus introduced of pANG1-23 produces a large amount of dihydrofolic acid reductase- angiotensin fused protein.

公开(公告)号：JPH01249763A

公开(公告)日：1989-10-05

申请号：JP7967488

申请日：1988-03-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： TANAKA YOSHIO ,  SAKURABA EIGO

IPC: C07D301/03

Abstract: PURPOSE:To readily obtain an optically active high-purity epoxy compound useful as an intermediate for various preformed chemical mediators and elements for information, by including an ethylenic unsaturated compound in cyclodextrin, dispersing and bringing the resultant substance into contact with an oxidizing agent. CONSTITUTION:An arbitrary organic group-containing ethylenic unsaturated compound not preventing inclusion in cyclodextrin and oxidation reaction is included in cyclodextrin, optionally dried, dispersed into any of various organic solvents or an aqueous solution optionally containing an organic solvent and catalytically reacted with an oxidizing agent well-known as an epoxidizing agent of an unsaturated compound preferably at -20-50 deg.C for 2-100hours to selectively obtain an optically active epoxy compound in high purity useful as an intermediate for ferroelectric liquid crystal, antitumor agent, antibiotic by using an inexpensive and repeatedly usable cyclic oligosaccharide free from toxicity.

公开(公告)号：JPH01230594A

公开(公告)日：1989-09-14

申请号：JP5717288

申请日：1988-03-10

Applicant: AGENCY IND SCIENCE TECHN

Inventor： FURUSAWA KIYOTAKA ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C07H19/06 ,  C07H1/00 ,  C07H19/16 ,  C07H23/00

Abstract: PURPOSE:To eliminate protecting groups completely under mild conditions in a shortened time, being very beneficial in nucleic acid synthesis, by allowing a trialkylammonium fluoride to act on specific cyclic silicon-protecting derivatives. CONSTITUTION:(A) Trialkylammonium fluoride is allowed to act on (B) cyclic silicon-protecting groups of formula I (R, R' are alkyl, cycloalkyl, aryl, aralkyl, alkaryl; R'' is H, hydroxyl, protected hydroxyl; B is monovalent residue of nucleic acid base or its derivative to effect hydrolysis whereby the compound of formula II is obtained.