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公开(公告)号：DK166685A
公开(公告)日：1986-07-08
申请号：DK166685
申请日：1985-04-12
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMANOBE TAKASHI , MITSUISHI YASUSHI , TAKASAKI YOSHIYUKI
Abstract: A method for the saccharification of a cellulosic material comprises the steps of culturing a microorganism of Acremonium cellulolyticus in a medium containing carbon sources and nitrogen sources, collecting a cellulolytic enzyme from the resultant culture broth, and causing the cellulolytic enzyme to act on the cellulosic material.
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公开(公告)号：DK164070C
公开(公告)日：1992-10-12
申请号：DK166685
申请日：1985-04-12
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMANOBE TAKASHI , MITSUISHI YASUSHI , TAKASAKI YOSHIYUKI
Abstract: A method for the saccharification of a cellulosic material comprises the steps of culturing a microorganism of Acremonium cellulolyticus in a medium containing carbon sources and nitrogen sources, collecting a cellulolytic enzyme from the resultant culture broth, and causing the cellulolytic enzyme to act on the cellulosic material.
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公开(公告)号：DK164070B
公开(公告)日：1992-05-04
申请号：DK166685
申请日：1985-04-12
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMANOBE TAKASHI , MITSUISHI YASUSHI , TAKASAKI YOSHIYUKI
Abstract: A method for the saccharification of a cellulosic material comprises the steps of culturing a microorganism of Acremonium cellulolyticus in a medium containing carbon sources and nitrogen sources, collecting a cellulolytic enzyme from the resultant culture broth, and causing the cellulolytic enzyme to act on the cellulosic material.
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公开(公告)号：DE3583603D1
公开(公告)日：1991-08-29
申请号：DE3583603
申请日：1985-04-10
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMANOBE TAKASHI , MITSUISHI YASUSHI , TAKASAKI YOSHIYUKI
Abstract: A method for the saccharification of a cellulosic material comprises the steps of culturing a microorganism of Acremonium cellulolyticus in a medium containing carbon sources and nitrogen sources, collecting a cellulolytic enzyme from the resultant culture broth, and causing the cellulolytic enzyme to act on the cellulosic material.
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公开(公告)号：DK166685D0
公开(公告)日：1985-04-12
申请号：DK166685
申请日：1985-04-12
Applicant: AGENCY IND SCIENCE TECHN
Inventor： YAMANOBE TAKASHI , MITSUISHI YASUSHI , TAKASAKI YOSHIYUKI
Abstract: A method for the saccharification of a cellulosic material comprises the steps of culturing a microorganism of Acremonium cellulolyticus in a medium containing carbon sources and nitrogen sources, collecting a cellulolytic enzyme from the resultant culture broth, and causing the cellulolytic enzyme to act on the cellulosic material.
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Patent Agency Ranking
公开(公告)号：JPH01171484A
公开(公告)日：1989-07-06
申请号：JP32846887
申请日：1987-12-25
Applicant: AGENCY IND SCIENCE TECHN
Inventor： MITSUISHI YASUSHI , YAMABE HITOSHI , YAGISAWA MITSUO , TAKASAKI YOSHIYUKI
Abstract: PURPOSE:To obtain xylooligosyl transferase A capable of carrying out xylooligosyl transfer using a xylooligosaccharide, etc., prepared from xylan as a donor, by cultivating a fungus of the genus Acremonium having the ability to produce xylooligosyl transferase A and collecting the above-mentioned transferase A from the resultant culture. CONSTITUTION:Xylooligosyl transferase A is produced by using a liquid or solid culture medium normally containing a vegetable biomass, such as xyloglucan or cellulose, as a carbon source and further a nitrogen source, such as nitrate or NH4 salt, and a small amount of a metallic salt with a fungus of the genus Acremonium. The cultivation in this case is aerobically carried out at 20-40 deg.C for about 2-15 days. Since the afore-mentioned transferase A is an extracellularly produced enzyme, a supernatant liquid prepared by filtration or centrifugation after the cultivation is used as a crude enzymic liquid. Enzymic powder is then obtained from the above-mentioned crude enzymic liquid by a well-known method, e.g., salting out with ammonium sulfate. Furthermore, since this enzyme is thermostable, heat treatment can be carried out at 65 deg.C and pH 4.9 to denature, precipitate and remove impurities without impairing activity of the above-mentioned transferase A. Thereby the aimed enzymic liquid having a high purity of the afore-mentioned transferase A is obtained.
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公开(公告)号：JPS61162179A
公开(公告)日：1986-07-22
申请号：JP58385
申请日：1985-01-07
Applicant: AGENCY IND SCIENCE TECHN
Inventor： TAKASAKI YOSHIYUKI , YAMABE HITOSHI , MITSUISHI YASUSHI
Abstract: PURPOSE:To increase the productivity of cellulase in the culture of a cellulase- producing mold, by carrying out the culture in the presence of betaine. CONSTITUTION:A cellulase-producing strain belonging to Acremonium genus, Trichoderma genus, Aspergillus genus, Penicillium genus, etc. such as Acremonium cellolyticus (FERM BP-685) is cultured in a medium containing a cellulose-containing component such as cellulose, avicel, etc. as a carbon source and added with a nitrogen source, metallic salt, etc. In the above process, the medium is added with about 0.1-1% betaine. The betaine may be the one separated from animals or vegetables or produced from glycine by synthetic means. After the cultivation by conventional method, the objective cellulase is separated from the culture liquid.
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公开(公告)号：JPH1060005A
公开(公告)日：1998-03-03
申请号：JP21449996
申请日：1996-08-14
Applicant: AGENCY IND SCIENCE TECHN
Inventor： MITSUISHI YASUSHI
Abstract: PROBLEM TO BE SOLVED: To provide a straight sugar chain molecular weight marker having a single molecular weight of >=10,000 free from molecular weight dispersion which is difficult to produce by conventional technique and provide a process for the production of the marker. SOLUTION: This process comprises a step to partially decompose a straight- chain sugar consisting of maltotriose having a regular subunit structure with its decomposition enzyme, a step to remove a component having a molecular weight lower than a prescribed level from the partially decomposed product and a step to separate and purify the obtained product free from the component having a molecular weight lower than a prescribed level by a normal-phase high-performance liquid chromatography containing an amide-bonded silica- packed column and
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公开(公告)号：JPH0759565A
公开(公告)日：1995-03-07
申请号：JP23425993
申请日：1993-08-26
Applicant: AGENCY IND SCIENCE TECHN
Inventor： MITSUISHI YASUSHI , KOSUGI YOSHIJI
Abstract: PURPOSE:To obtain the xyloglucan-decomposing enzyme not acting on cellulose but specifically acting only on xyloglucan to utilize the enzyme for the elucida tion of the structure and function of the xyloglucan which is a biological poly mer having an important role on e.g. the elongation and differentiation of plants. CONSTITUTION:This method for producing the xyloglucan-decomposing enzyme comprises culturing M451 strain belonging to the genus Penicilfum and subsequently removing the coexisting endo type cellulase-like enzymatic activity front the obtained crude enzyme solution with chromatography, etc.
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公开(公告)号：JPS61162181A
公开(公告)日：1986-07-22
申请号：JP349085
申请日：1985-01-11
Applicant: AGENCY IND SCIENCE TECHN
Inventor： MITSUISHI YASUSHI , YAMABE HITOSHI , TAKASAKI YOSHIYUKI
Abstract: PURPOSE:To collect thermostable xylanase from a culture mixture, by cultivating a microorganism belonging to the genus Acremonium, capable of producing thermostable xylanase. CONSTITUTION:A microorganism such as Acromonium cellulolyticus TN (FERMBP-685), etc. belonging to the genus Acremonium, capable of producing thermostable xylanase, is cultivated. It is cultivated aerobically in a medium containing a carbon source such as xylan, cellulose, bagasse, etc., a nitrogen source such as a nitrate, etc. and a metallic salt. A supernatant liquid or an extracted solution obtained after the cultivation is used as a crude enzyme solution. Crude enzyme powder can be obtained by acetone precipitation method, etc., impure protein is denatured and precipitated by heat treatment and removed, to give an enzyme solution having only xylanase activity.
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