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    PRODUCTION OF XYLOOLIGOSACCHARIDE DERIVATIVE
    6.
    发明专利
    PRODUCTION OF XYLOOLIGOSACCHARIDE DERIVATIVE 失效

    公开(公告)号：JPH01171492A

    公开(公告)日：1989-07-06

    申请号：JP32847087

    申请日：1987-12-25

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： MITSUISHI YASUSHI ,  YAMABE HITOSHI ,  YAGISAWA MITSUO ,  TAKASAKI YOSHIYUKI

    IPC: C12P19/04 ,  C12N9/10 ,  C12P19/00 ,  C12P19/12 ,  C12R1/645

    Abstract: PURPOSE:To obtain a xylooligosaccharide derivative useful in various starch industries, in high efficiency, by contacting a specific enzyme with xylan or hydrolyzed xylan in the presence of an alcohol. CONSTITUTION:A microbial strain of genus Acremonium (e.g. Acremonium cellulolyticus) capable of producing xylooligosyl transferase A is cultured in a medium containing a vegetable biomass (e.g. bagasse) as a carbon source and a nitrogen source (e.g. peptone) at 20-40 deg.C for 2-15 days under aerobic condition. The cultured product is heat-treated and purified to obtain a xylooligosyl transferase A having a molecular weight of 51,000, an isoelectric point of about 5.0, a working pH of 3-6 and an optimum working temperature of 50 deg.C. The enzyme A is added to a reaction liquid containing xylotetraose and a 1-10C straight-chain alkyl alcohol, etc., and made to react at about 45 deg.C for about 2hr to obtain the objective xylooligosaccharide derivative.

    NOVEL XYLOOLIGOSYL TRANSFERASE
    7.
    发明专利
    NOVEL XYLOOLIGOSYL TRANSFERASE 失效

    公开(公告)号：JPH01171485A

    公开(公告)日：1989-07-06

    申请号：JP32846987

    申请日：1987-12-25

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： MITSUISHI YASUSHI ,  YAMABE HITOSHI ,  YAGISAWA MITSUO ,  TAKASAKI YOSHIYUKI

    IPC: C12N9/10 ,  C12R1/645

    Abstract: PURPOSE:To provide a novel xylooligosyl transferase having specific molecular weight, isoelectric point, optimum temperature in the case of using a sugar as a receptor, etc., capable of transferring a xylooligosaccharide residue having a polymerization degree of >=2 and effective in performing xylooligosyl transfer reaction using xylooligosaccharide as a donor. CONSTITUTION:The objective novel xylooligosyl transferase provided in the present invention is effective in producing a useful substance by the sugar trans fer reaction using an enzyme catalyst from xylan or hydrolyzed xylan which is an important biomass raw material and in expanding the utilization range of xylan. An effective microbial strain capable of producing said transferase is e.g. Acremonium cellulolyticus. The transferase has the following enzymatic properties. Molecular weight, about 51,000; isoelectric point, pH 5.0; optimum temperature mentioned above, about 80 deg.C; optimum working pH in the case of using a sugar as a receptor, about 4.9; etc.

    PRODUCTION OF XYLOOLIGOSYL TRANSFERASE
    8.
    发明专利
    PRODUCTION OF XYLOOLIGOSYL TRANSFERASE 失效

    公开(公告)号：JPH01171484A

    公开(公告)日：1989-07-06

    申请号：JP32846887

    申请日：1987-12-25

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： MITSUISHI YASUSHI ,  YAMABE HITOSHI ,  YAGISAWA MITSUO ,  TAKASAKI YOSHIYUKI

    IPC: C12N9/10 ,  C12R1/645

    Abstract: PURPOSE:To obtain xylooligosyl transferase A capable of carrying out xylooligosyl transfer using a xylooligosaccharide, etc., prepared from xylan as a donor, by cultivating a fungus of the genus Acremonium having the ability to produce xylooligosyl transferase A and collecting the above-mentioned transferase A from the resultant culture. CONSTITUTION:Xylooligosyl transferase A is produced by using a liquid or solid culture medium normally containing a vegetable biomass, such as xyloglucan or cellulose, as a carbon source and further a nitrogen source, such as nitrate or NH4 salt, and a small amount of a metallic salt with a fungus of the genus Acremonium. The cultivation in this case is aerobically carried out at 20-40 deg.C for about 2-15 days. Since the afore-mentioned transferase A is an extracellularly produced enzyme, a supernatant liquid prepared by filtration or centrifugation after the cultivation is used as a crude enzymic liquid. Enzymic powder is then obtained from the above-mentioned crude enzymic liquid by a well-known method, e.g., salting out with ammonium sulfate. Furthermore, since this enzyme is thermostable, heat treatment can be carried out at 65 deg.C and pH 4.9 to denature, precipitate and remove impurities without impairing activity of the above-mentioned transferase A. Thereby the aimed enzymic liquid having a high purity of the afore-mentioned transferase A is obtained.

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