公开(公告)号：WO2005098430A3

公开(公告)日：2005-12-15

申请号：PCT/US2005008866

申请日：2005-03-16

Applicant: AMNIS CORP ,  GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

Inventor： GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

IPC: G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/56966

Abstract: The use of a multi-spectral imaging system, cell compartment markers, and molecular probes in a method for measuring movement of molecules within a cell by correlation analysis is provided, including measuring molecular movement to a particular compartment in adherent and non-adherent cells, e.g. in response to biological stimuli. A compartment in the cell is defined by the image of a specific compartment marker, e.g., a nuclear fluorescent stain. Molecule location is provided by a probe labeled with a different fluorochrome. A mask is generated based on the compartmental marker, and a correlation measurement is made between the locations of the molecular probe and the compartment marker. The correlation value between the regions defined by the compartment mask and molecular probe gives a quantitative measurement of the translocation of the molecule. The use of only a single masking function simplifies measurement of molecular translocation within a cell.

Abstract translation: 提供了通过相关分析在测量细胞内分子运动的方法中使用多光谱成像系统，细胞室标记物和分子探针，包括测量分子对粘附细胞和非贴壁细胞中特定隔室的运动， 例如 响应生物刺激。 细胞中的隔室由特定区室标记物的图像限定，例如核荧光染色剂。 分子位置由标记有不同荧光染料的探针提供。 基于区室标记产生掩模，并且在分子探针和隔室标记的位置之间进行相关性测量。 由隔室掩模和分子探针限定的区域之间的相关值给出了分子易位的定量测量。 仅使用单一屏蔽功能简化了细胞内分子易位的测量。

公开(公告)号：CA2598602A1

公开(公告)日：2005-10-20

申请号：CA2598602

申请日：2005-03-16

Applicant: AMNIS CORP

Inventor： HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J ,  FROST KEITH ,  GEORGE THADDEUS C ,  BASIJI DAVID A

IPC: G01N33/50 ,  G01N21/64 ,  G01N33/569 ,  G01N33/58

公开(公告)号：CA2596101A1

公开(公告)日：2005-09-29

申请号：CA2596101

申请日：2005-03-16

Applicant: AMNIS CORP

Inventor： SEO MICHAEL J ,  GEORGE THADDEUS C ,  HALL BRIAN E ,  BASIJI DAVID A ,  ORTYN WILLIAM E ,  ZIMMERMAN CATHLEEN A ,  MORRISSEY PHILIP J

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/50

Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.