公开(公告)号：WO2005098430A3

公开(公告)日：2005-12-15

申请号：PCT/US2005008866

申请日：2005-03-16

Applicant: AMNIS CORP ,  GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

Inventor： GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

IPC: G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/56966

Abstract: The use of a multi-spectral imaging system, cell compartment markers, and molecular probes in a method for measuring movement of molecules within a cell by correlation analysis is provided, including measuring molecular movement to a particular compartment in adherent and non-adherent cells, e.g. in response to biological stimuli. A compartment in the cell is defined by the image of a specific compartment marker, e.g., a nuclear fluorescent stain. Molecule location is provided by a probe labeled with a different fluorochrome. A mask is generated based on the compartmental marker, and a correlation measurement is made between the locations of the molecular probe and the compartment marker. The correlation value between the regions defined by the compartment mask and molecular probe gives a quantitative measurement of the translocation of the molecule. The use of only a single masking function simplifies measurement of molecular translocation within a cell.

Abstract translation: 提供了通过相关分析在测量细胞内分子运动的方法中使用多光谱成像系统，细胞室标记物和分子探针，包括测量分子对粘附细胞和非贴壁细胞中特定隔室的运动， 例如 响应生物刺激。 细胞中的隔室由特定区室标记物的图像限定，例如核荧光染色剂。 分子位置由标记有不同荧光染料的探针提供。 基于区室标记产生掩模，并且在分子探针和隔室标记的位置之间进行相关性测量。 由隔室掩模和分子探针限定的区域之间的相关值给出了分子易位的定量测量。 仅使用单一屏蔽功能简化了细胞内分子易位的测量。

公开(公告)号：WO0231583A8

公开(公告)日：2002-07-04

申请号：PCT/US0131930

申请日：2001-10-12

Applicant: AMNIS CORP ,  ORTYN WILLIAM E ,  BASIJI DAVID A ,  PERRY DAVID J

Inventor： ORTYN WILLIAM E ,  BASIJI DAVID A ,  PERRY DAVID J

IPC: C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/14 ,  G01N21/03 ,  G01N21/05 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G02B27/10 ,  G01J3/14

CPC classification number: G02B27/1086 ,  B01J2219/00274 ,  C07K1/047 ,  C12Q1/6816 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/031 ,  G01N21/05 ,  G01N2015/0294 ,  G01N2015/1443 ,  G01N2015/1452 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2015/1488 ,  G01N2021/0346 ,  G01N2021/052 ,  G01N2021/058 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  H04N5/2254 ,  H04N5/23212 ,  C12Q2563/179 ,  C12Q2563/107

Abstract: A system and method for high numeric aperture imaging systems includes a splitter (110), a defocusing system (126), and a combiner (114). The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and reflected portion such that the transmitted portion and reflected are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.

Abstract translation: 用于高数值孔径成像系统的系统和方法包括分离器（110），散焦系统（126）和组合器（114）。 分光器反射一部分收集的光并透射另一部分收集的光。 散焦系统被配置为修改所收集的光的透射部分或反射部分的光焦度。 组合器相对于机械角度取向。 组合器将发送部分和反射部分的部分重新组合，使得发送部分和反射随后被发送，基于组合器的机械取向的机械角度，光学分离角度被分离。 使用各种其他实现方式来保持所涉及的成像系统的焦点。

公开(公告)号：CA2395627C

公开(公告)日：2005-04-26

申请号：CA2395627

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM E ,  BASIJI DAVID A

IPC: C12Q1/02 ,  G01J3/28 ,  G01N15/02 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/48 ,  G02B7/28 ,  G02B27/00 ,  G02B27/14 ,  G06K9/00 ,  G06T1/00 ,  G01J3/18 ,  G01J3/30

Abstract: Light (30) from an object (24) such as a cell moving through an imaging syst em (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light (30) can be emitted from a luminous object (24) or can be light from a light source that has been scattered by the object or can be a fluorescent emission by one or more FISH probes, frequently used to detect substances within cells. Further, light that is absorbed or reflected by the object can also be used to produce images for determining specific characteristics of the object. The movement of the object matches the rate at which a signal is read from the TDI detector. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and spectrally dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：CA2401614A1

公开(公告)日：2001-07-26

申请号：CA2401614

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： BASIJI DAVID A ,  ORTYN WILLIAM E

IPC: G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G02B7/28 ,  G02B27/14 ,  G01J3/12 ,  G01J3/51

Abstract: Light from an object (24), such as a cell, moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：CA2395627A1

公开(公告)日：2001-07-26

申请号：CA2395627

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： BASIJI DAVID A ,  ORTYN WILLIAM E

IPC: C12Q1/02 ,  G01J3/28 ,  G01N15/02 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/48 ,  G02B7/28 ,  G02B27/00 ,  G02B27/14 ,  G06K9/00 ,  G06T1/00 ,  G01J3/18 ,  G01J3/30

Abstract: Light (30) from an object (24) such as a cell moving through an imaging syst em (20) is collected and dispersed so that it can be imaged onto a time delay a nd integration (TDI) detector (44). The light (30) can be emitted from a lumino us object (24) or can be light from a light source that has been scattered by t he object or can be a fluorescent emission by one or more FISH probes, frequent ly used to detect substances within cells. Further, light that is absorbed or reflected by the object can also be used to produce images for determining specific characteristics of the object. The movement of the object matches t he rate at which a signal is read from the TDI detector. Multiple objects passi ng through the imaging system (20) can be imaged, producing both scatter images and spectrally dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：CA2401614C

公开(公告)日：2009-06-02

申请号：CA2401614

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM E ,  BASIJI DAVID A

IPC: G01J3/12 ,  G01D5/28 ,  G01J3/26 ,  G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/52 ,  G02B5/26 ,  G02B7/28 ,  G02B27/14

Abstract: Light from an object (24), such as a cell, moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：CA2598602A1

公开(公告)日：2005-10-20

申请号：CA2598602

申请日：2005-03-16

Applicant: AMNIS CORP

Inventor： HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J ,  FROST KEITH ,  GEORGE THADDEUS C ,  BASIJI DAVID A

IPC: G01N33/50 ,  G01N21/64 ,  G01N33/569 ,  G01N33/58

公开(公告)号：CA2596101A1

公开(公告)日：2005-09-29

申请号：CA2596101

申请日：2005-03-16

Applicant: AMNIS CORP

Inventor： SEO MICHAEL J ,  GEORGE THADDEUS C ,  HALL BRIAN E ,  BASIJI DAVID A ,  ORTYN WILLIAM E ,  ZIMMERMAN CATHLEEN A ,  MORRISSEY PHILIP J

IPC: G01N15/14 ,  G01N21/64 ,  G01N33/50

Abstract: Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.

公开(公告)号：AU1315702A

公开(公告)日：2002-04-22

申请号：AU1315702

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM E ,  BASIJI DAVID A ,  PERRY DAVID J

IPC: C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N15/02 ,  G01N15/14 ,  G01N21/03 ,  G01N21/05 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G02B27/10 ,  G01J3/14

Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.