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    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING
    1.
    发明申请
    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING 审中-公开
    用于校正多通道成像的波形和空间分辨率的方法和装置

    公开(公告)号：WO02086416A3

    公开(公告)日：2003-02-20

    申请号：PCT/US0214979

    申请日：2002-04-24

    Applicant: AMNIS CORP

    Inventor： RILEY JAMES ,  FROST KEITH

    IPC: A61B6/00 ,  G01B20060101 ,  G06K9/00 ,  G06K9/03 ,  G06K9/32 ,  G06K9/40 ,  G06K9/68 ,  G06T5/00 ,  G06T5/50 ,  G06T7/00

    CPC classification number: G06K9/00503 ,  G01J3/28 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01N15/147 ,  G01N15/1475 ,  G06K9/00127 ,  G06T5/50 ,  G06T7/32 ,  G06T7/33 ,  G06T2207/10036

    Abstract: A multichannel imaging system generates an ensemble of images for each field of view of an object (401). Each image in the ensemble is intended to contain information from only one source among a plurality of sources for the object. However, due to crosstalk, at least a portion of the signal from a first source appears in a channel intended for a second source (404). Because the accuracy of the correction will be degraded if the images in an ensemble are spatially misaligned with respect to one another, the spatial offset between images is determined (408) and a correction is applied to substantially eliminate the offset. Then, a correction to the signals is determined to substantially reduce the contributions to the signal in a channel from the signals in other channels. The signal processing can be employed to process the output signals for each of a plurality of different disclosed imaging systems (409).

    Abstract translation: 多通道成像系统为对象的每个视场生成图像集合（401）。 集合中的每个图像旨在包含来自对象的多个源中的一个源的信息。 然而，由于串扰，来自第一源的信号的至少一部分出现在用于第二源（404）的通道中。 因为如果一个集合中的图像相对于彼此在空间上不对准，校正的精度将被降低，则确定图像之间的空间偏移（408），并应用校正以基本上消除偏移。 然后，确定对信号的校正，以从其它通道中的信号基本上减少对信道中的信号的贡献。 可以采用信号处理来处理多个不同公开的成像系统（409）中的每一个的输出信号。

    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING
    2.
    发明专利
    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING 未知

    公开(公告)号：CA2445044C

    公开(公告)日：2011-02-15

    申请号：CA2445044

    申请日：2002-04-24

    Applicant: AMNIS CORP

    Inventor： RILEY JAMES ,  FROST KEITH

    IPC: G01N21/84 ,  A61B6/00 ,  G01B20060101 ,  G01D3/032 ,  G01N15/10 ,  G06K9/00 ,  G06K9/03 ,  G06K9/32 ,  G06K9/40 ,  G06K9/68 ,  G06T5/00 ,  G06T5/50 ,  G06T7/00 ,  H04N5/21

    Abstract: A multichannel imaging system generates an ensemble of images for each field of view of an object (401). Each image in the ensemble is intended to contain information from only one source among a plurality of sources for the object. However, due to crosstalk, at least a portion of the signal from a first source appears in a channel intended for a second source (404). Because the accuracy of the correction will be degraded if the images in an ensemble are spatially misaligned with respect to one another, the spatial offset between images is determined (408) and a correction is applied to substantially eliminate the offset. Then, a correction to the signals is determined to substantially reduce the contributions to the signal in a channel from the signals in other channels. The signal processing can be employed to process the output signals for each of a plurality of different disclosed imaging systems (409).

    IMAGE BASED QUANTITATION OF MOLECULAR TRANSLOCATION
    3.
    发明申请
    IMAGE BASED QUANTITATION OF MOLECULAR TRANSLOCATION 审中-公开
    基于图像的分子量转换定量

    公开(公告)号：WO2005098430A3

    公开(公告)日：2005-12-15

    申请号：PCT/US2005008866

    申请日：2005-03-16

    Applicant: AMNIS CORP ,  GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

    Inventor： GEORGE THADDEUS C ,  BASIJI DAVID A ,  FROST KEITH ,  HALL BRIAN E ,  ORTYN WILLIAM E ,  SEO MICHAEL J

    IPC: G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58

    CPC classification number: G01N33/56966

    Abstract: The use of a multi-spectral imaging system, cell compartment markers, and molecular probes in a method for measuring movement of molecules within a cell by correlation analysis is provided, including measuring molecular movement to a particular compartment in adherent and non-adherent cells, e.g. in response to biological stimuli. A compartment in the cell is defined by the image of a specific compartment marker, e.g., a nuclear fluorescent stain. Molecule location is provided by a probe labeled with a different fluorochrome. A mask is generated based on the compartmental marker, and a correlation measurement is made between the locations of the molecular probe and the compartment marker. The correlation value between the regions defined by the compartment mask and molecular probe gives a quantitative measurement of the translocation of the molecule. The use of only a single masking function simplifies measurement of molecular translocation within a cell.

    Abstract translation: 提供了通过相关分析在测量细胞内分子运动的方法中使用多光谱成像系统，细胞室标记物和分子探针，包括测量分子对粘附细胞和非贴壁细胞中特定隔室的运动， 例如 响应生物刺激。 细胞中的隔室由特定区室标记物的图像限定，例如核荧光染色剂。 分子位置由标记有不同荧光染料的探针提供。 基于区室标记产生掩模，并且在分子探针和隔室标记的位置之间进行相关性测量。 由隔室掩模和分子探针限定的区域之间的相关值给出了分子易位的定量测量。 仅使用单一屏蔽功能简化了细胞内分子易位的测量。

    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS
    4.
    发明申请
    EXTENDED DEPTH OF FIELD IMAGING FOR HIGH SPEED OBJECT ANALYSIS 审中-公开
    用于高速物体分析的现场成像的扩展深度

    公开(公告)号：WO2007067999A3

    公开(公告)日：2007-11-29

    申请号：PCT/US2006061871

    申请日：2006-12-11

    Applicant: AMNIS CORP ,  ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

    Inventor： ORTYN WILLIAM ,  BASIJI DAVID ,  FROST KEITH ,  LIANG LUCHUAN ,  BAUER RICHARD ,  HALL BRIAN ,  PERRY DAVID

    IPC: G02F1/01

    CPC classification number: G02B27/0075 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/1452 ,  G02B21/365 ,  G02B27/0012

    Abstract: A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

    Abstract translation: 改进了高速，高分辨率流动成像系统，以实现扩展的景深成像。 光学失真元件被引入到流动成像系统中。 来自诸如单元的物体的光被失真元件扭曲，使得成像系统的点扩散函数（PSF）在扩展的景深范围内是不变的。 失真光被光谱分散，并且分散的光被用于同时产生多个图像。 检测图像，并且使用图像处理来通过补偿失真来增强检测到的图像，以实现对象的扩展景深图像。 后图像处理优选地涉及去卷积，并且需要由光学失真元件修改的成像系统的PSF的知识。

    Computational methods for the segmentation of images of objects from background in a flow imaging instrument
    8.
    发明专利
    Computational methods for the segmentation of images of objects from background in a flow imaging instrument 未知

    公开(公告)号：AU2002319621A1

    公开(公告)日：2003-03-03

    申请号：AU2002319621

    申请日：2002-07-17

    Applicant: AMNIS CORP

    Inventor： RILEY JAMES ,  FROST KEITH

    IPC: G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G02B27/00 ,  G06K9/00 ,  G06K9/32

    Abstract: In automated computation-based interpretation of images, the accuracy and reliability of the detection and delineation of objects, known as "object segmentation," is implemented so as to provide efficient performance. In a multi-step process, objects are first detected and captured into regions of interest (ROIs). Sets of pixels belonging to respective objects are then identified. Preferably object detection is achieved using both a two-dimensional (2D) low pass filter and a 2D edge enhancement filter. Two different gradient based edge enhancement filters are disclosed. One embodiment of the invention defines a (ROI) by first determining the center of objects by executing a plurality of decimations on the filtered image data, and then establishing object boundaries. In a second embodiment the ROI is defined by generating an amplitude histogram of the filtered image data, and for histograms exceeding a threshold determining by pixel which rows are to be included in the ROI.

    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING
    9.
    发明专利
    METHOD AND APPARATUS FOR CORRECTING CROSSTALK AND SPATIAL RESOLUTION FOR MULTICHANNEL IMAGING 未知

    公开(公告)号：CA2445044A1

    公开(公告)日：2002-10-31

    申请号：CA2445044

    申请日：2002-04-24

    Applicant: AMNIS CORP

    Inventor： FROST KEITH ,  RILEY JAMES

    IPC: A61B6/00 ,  G01B20060101 ,  G06K9/00 ,  G06K9/03 ,  G06K9/32 ,  G06K9/40 ,  G06K9/68 ,  G06T5/00 ,  G06T5/50 ,  G06T7/00

    Abstract: A multichannel imaging system generates an ensemble of images for each field of view of an object (401). Each image in the ensemble is intended to contai n information from only one source among a plurality of sources for the object . However, due to crosstalk, at least a portion of the signal from a first source appears in a channel intended for a second source (404). Because the accuracy of the correction will be degraded if the images in an ensemble are spatially misaligned with respect to one another, the spatial offset between images is determined (408) and a correction is applied to substantially eliminate the offset. Then, a correction to the signals is determined to substantially reduce the contributions to the signal in a channel from the signals in other channels. The signal processing can be employed to process the output signals for each of a plurality of different disclosed imaging systems (409).

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