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公开(公告)号：WO2004066189B1
公开(公告)日：2004-11-04
申请号：PCT/US2004001116
申请日：2004-01-16
Applicant: AMNIS CORP , ORTYN WILLIAM , BASIJI DAVID , FROST KEITH , HALL BRIAN , SEO MICHAEL
Inventor： ORTYN WILLIAM , BASIJI DAVID , FROST KEITH , HALL BRIAN , SEO MICHAEL
CPC classification number: G01N15/1012 , G01N15/1459 , G01N15/1463 , G01N15/147 , G01N15/1475 , G01N2015/1018 , G01N2015/1025 , G01N2015/1075 , G01N2015/144 , G01N2015/1443 , G01N2015/1447 , G01N2015/1452 , G01N2015/1465 , G01N2015/1472 , G01N2015/1477 , G01N2015/1497 , G02B27/0012 , G06T7/20
Abstract: When utilized in a flow imaging instrument, calibration beads provide a known data source that can be employed in various self diagnostic, calibration, and quality metric applications for the both the optical system of the flow imaging instrument, as well as the flow cell of the flow imaging instrument. Such data can be used to determine point spread functions associated with an imaging system, to determine a sensitivity of an imaging system, and to determine a focal point of the imaging system. Imagery collected from calibration beads can be used to determine core size and stability and TDI/flow speed synchronization. Calibration beads can be beneficially employed to enable stable system operation, even when very low sample concentration, or very small sample sizes are to be analyzed.
Abstract translation: 当用于流动成像仪器时，校准珠粒提供了一种已知的数据源，可用于各种自诊断，校准和质量度量应用，用于流动成像仪器的光学系统以及流动成像仪器的流动池 流量成像仪。 这样的数据可以用于确定与成像系统相关联的点扩散函数，确定成像系统的灵敏度并且确定成像系统的焦点。 从校准珠收集的图像可用于确定核心大小和稳定性以及TDI /流速同步。 即使样品浓度非常低或者要分析非常小的样品量，校准珠也可以有利地用于稳定的系统操作。
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公开(公告)号：AT538138T
公开(公告)日：2012-01-15
申请号：AT05760334
申请日：2005-03-16
Applicant: AMNIS CORP
Inventor： GEORGE THADDEUS , BASIJI DAVID , FROST KEITH , HALL BRIAN , ORTYN WILLIAM , SEO MICHAEL
IPC: C07K16/28 , G01N21/64 , G01N33/50 , G01N33/569 , G01N33/58
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公开(公告)号：WO2004092781A3
公开(公告)日：2005-05-06
申请号：PCT/US2004011030
申请日：2004-04-09
Applicant: AMNIS CORP , ORTYN WILLIAM , SEO MICHAEL , BASIJI DAVID , FROST KEITH , PERRY DAVID
Inventor： ORTYN WILLIAM , SEO MICHAEL , BASIJI DAVID , FROST KEITH , PERRY DAVID
CPC classification number: G01N15/147 , G01N2015/1075 , G01N2015/1452
Abstract: A pair of optical gratings (126, 134) are used to modulate light from an object (102), and the modulated light from either optical is used to determine the velocity of the object. Each optical grating is offset from a reference focal point by the same distance, one grating being offset in a positive direction, the other in a negative direction. Signals produced in response to the modulated light can be processed to determine a direction in which a primary collection lens (110) should be moved in order to improve a focus of the imaging system on the object. The lens is moved incrementally in the direction so determined, and the process is repeated until an optimal focus is achieved. In a preferred embodiment, the signals are weighted, so that the optical grating disposed closest to the optimal focus position contributes the most to velocity detection.
Abstract translation: 一对光栅（126,134）用于调制来自物体（102）的光，并且来自任一光学的调制光用于确定物体的速度。 每个光栅从参考焦点偏移相同的距离，一个光栅在正方向上偏移，另一个光栅在负方向上偏移。 可以处理响应于调制光产生的信号以确定主收集透镜（110）应该移动的方向，以便改善成像系统对物体的焦点。 透镜在如此确定的方向上逐渐移动，并重复该过程，直到达到最佳聚焦。 在优选实施例中，对信号进行加权，使得最靠近最佳聚焦位置布置的光栅最有助于速度检测。
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公开(公告)号：WO2004066189A3
公开(公告)日：2004-09-23
申请号：PCT/US2004001116
申请日：2004-01-16
Applicant: AMNIS CORP , ORTYN WILLIAM , BASIJI DAVID , FROST KEITH , HALL BRIAN , SEO MICHAEL
Inventor： ORTYN WILLIAM , BASIJI DAVID , FROST KEITH , HALL BRIAN , SEO MICHAEL
CPC classification number: G01N15/1012 , G01N15/1459 , G01N15/1463 , G01N15/147 , G01N15/1475 , G01N2015/1018 , G01N2015/1025 , G01N2015/1075 , G01N2015/144 , G01N2015/1443 , G01N2015/1447 , G01N2015/1452 , G01N2015/1465 , G01N2015/1472 , G01N2015/1477 , G01N2015/1497 , G02B27/0012 , G06T7/20
Abstract: When utilized in a flow imaging instrument, calibration beads provide a known data source that can be employed in various self diagnostic, calibration, and quality metric applications for the both the optical system of the flow imaging instrument, as well as the flow cell of the flow imaging instrument. Such data can be used to determine point spread functions associated with an imaging system, to determine a sensitivity of an imaging system, and to determine a focal point of the imaging system. Imagery collected from calibration beads can be used to determine core size and stability and TDI/flow speed synchronization. Calibration beads can be beneficially employed to enable stable system operation, even when very low sample concentration, or very small sample sizes are to be analyzed.
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