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    CONTAINER FOR NUCLEIC ACID EXTRACTION, METHOD OF CLEANING SOLID MATRIX AND RELEVANT CLEANING MECHANISM, AND METHOD OF PURIFYING NUCLEIC ACID
    1.
    发明申请
    CONTAINER FOR NUCLEIC ACID EXTRACTION, METHOD OF CLEANING SOLID MATRIX AND RELEVANT CLEANING MECHANISM, AND METHOD OF PURIFYING NUCLEIC ACID 审中-公开
    用于核酸提取的容器，清洁固体基质的方法和相关的清洁机理，以及净化核酸的方法

    公开(公告)号：WO2005118803A9

    公开(公告)日：2007-03-15

    申请号：PCT/JP2005010078

    申请日：2005-06-01

    Applicant: ARKRAY INC ,  KAMATA TATSUO ,  OHTA SHINICHI ,  EGAWA KOUJI

    Inventor： KAMATA TATSUO ,  OHTA SHINICHI ,  EGAWA KOUJI

    IPC: C12N15/09 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  G01N1/34 ,  G01N1/40

    CPC classification number: B01L3/5085 ,  B01L3/502 ,  B01L2200/0631 ,  B01L2300/0609 ,  G01N1/34 ,  G01N1/405

    Abstract: Container for nucleic acid extraction (2) comprising nucleic acid extraction element (20) for extracting of a target nucleic acid from a sample and for adhesion of the extracted nucleic acid and, provided separate from the nucleic acid extraction element (20), a container frame equipped with accommodation vessel (27) for accommodating of the nucleic acid extraction element (20). The nucleic acid extraction element (20) is constructed so as to include, for example, solid matrix (23) for carrying of the target nucleic acid and holding member (20) for holding of the solid matrix (23). Preferably, the solid matrix (23) is held in a form sloping against the vertical axis of the holding member (20).

    Abstract translation: 用于核酸提取的容器（2），其包含用于从样品中提取靶核酸并用于粘附所提取的核酸的核酸提取元件（20），并且与所述核酸提取元件（20）分开提供容器 框架配备有用于容纳核酸提取元件（20）的容纳容器（27）。 核酸提取元件（20）被构造为包括例如用于携带用于保持固体基质（23）的靶核酸和保持构件（20）的固体基质（23）。 优选地，固体基质（23）以与保持构件（20）的垂直轴线倾斜的形式保持。

    2.
    发明专利
    未知

    公开(公告)号：AT553214T

    公开(公告)日：2012-04-15

    申请号：AT08711870

    申请日：2008-02-24

    Applicant: ARKRAY INC

    Inventor： KAMATA TATSUO ,  TAKAGI TAKESHI

    IPC: C12Q1/00 ,  C12Q1/28

    Abstract: The present invention relates to a method, a reagent and a device for measuring a substrate concentration based on an amount of hydrogen peroxide generated from a substrate. In the present invention, a suppressing agent for suppressing a reaction between the hydrogen peroxide and an inhibitor is added. As the suppressing agent, an azide compound such as sodium azide or a nitrite compound such as sodium nitrite is used. In the invention, a supporting electrolyte, such as sodium chloride or potassium chloride may be further added.

    METHOD OF EFFECTING LYSIS OF ACID-FAST BACTERIA AND METHOD OF PERFORMING GENE AMPLIFICATION OR DETECTION THEREWITH
    3.
    发明专利
    METHOD OF EFFECTING LYSIS OF ACID-FAST BACTERIA AND METHOD OF PERFORMING GENE AMPLIFICATION OR DETECTION THEREWITH 未知

    公开(公告)号：AU2003242353A1

    公开(公告)日：2003-12-02

    申请号：AU2003242353

    申请日：2003-05-21

    Applicant: ARKRAY INC

    Inventor： KAMATA TATSUO ,  IZUMIZAWA YUJI

    IPC: C12N1/06 ,  C12N15/10 ,  C12Q1/68

    Abstract: A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96 DEG C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.

    4.
    发明专利
    未知

    公开(公告)号：DE60329676D1

    公开(公告)日：2009-11-26

    申请号：DE60329676

    申请日：2003-05-21

    Applicant: ARKRAY INC

    Inventor： KAMATA TATSUO ,  IZUMIZAWA YUJI

    IPC: C12N1/06 ,  C12N15/10 ,  C12Q1/68

    Abstract: A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96 DEG C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.

    5.
    发明专利
    未知

    公开(公告)号：AT445697T

    公开(公告)日：2009-10-15

    申请号：AT03730537

    申请日：2003-05-21

    Applicant: ARKRAY INC

    Inventor： KAMATA TATSUO ,  IZUMIZAWA YUJI

    IPC: C12N1/06 ,  C12N15/10 ,  C12Q1/68

    Abstract: A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96 DEG C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.

    Method for collecting microorganism and method for amplifying or detecting gene using the same
    10.
    发明专利
    Method for collecting microorganism and method for amplifying or detecting gene using the same 审中-公开
    用于收集微生物的方法和使用其放大或检测基因的方法

    公开(公告)号：JP2004180551A

    公开(公告)日：2004-07-02

    申请号：JP2002349587

    申请日：2002-12-02

    Applicant: Arkray Inc ,  アークレイ株式会社

    Inventor： KAMATA TATSUO ,  IZUMISAWA YUJI ,  MURAKAMI ATSUSHI

    IPC: C12Q1/68 ,  C12N1/02 ,  C12R1/265

    Abstract: PROBLEM TO BE SOLVED: To provide a method for simply collecting microorganisms in a short time without using a specific apparatus.
    SOLUTION: A liquid sample is placed on a collection filter 13 of a centrifugal tube 1 whose inside is divided into an upper part and a lower part by the collection tube 13 having pore diameters to catch the microorganisms. In this state, the centrifugal tube 1 is centrifuged to catch and collect the microorganisms on the filter 13. The pore diameters of the collection filter is 0.1-1.0μm. The centrifugation condition is, for example, one minute under 5,000G. A prefilter for catching an admixture may be arranged on the collection filter.
    COPYRIGHT: (C)2004,JPO&NCIPI

    Abstract translation: 待解决的问题：提供一种在不使用特定装置的情况下在短时间内简单地收集微生物的方法。 解决方案：将液体样品放置在离心管1的收集过滤器13上，离心管1的内部通过具有孔径的收集管13分成上部和下部，以捕获微生物。 在这种状态下，将离心管1离心以捕集并收集过滤器13上的微生物。收集过滤器的孔径为0.1-1.0μm。 离心条件例如在5,000G以下一分钟。 用于捕集混合物的预过滤器可以布置在收集过滤器上。 版权所有（C）2004，JPO＆NCIPI

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