Abstract:
An apparatus for use in a method of detecting and analysing fluorescent in-situ hybridisations (fig. 5) employing numerous chromosome paints (fig. 9) each labelled with a different fluorophore or combination of fluorophores, the apparatus being highly sensitive both in spatial and spectral resolutions (fig. 6) such that it is capable of simultaneous detection of dozens of fluorophores or combinations of fluorophores (fig. 7) so as to enable the detection of a complete set of fluorescently painted human chromosomes (fig. 10).
Abstract:
A method and an apparatus for analyzing an optical image of a scene to determine the spectral intensity of each pixel thereof, comprising: collecting incident light from the scene; passing the light through an interferometer wherein the light is first split into a finite plurality of coherent beams which travel along different optical paths and then recombine to interfere with each other, the interferometer thereby outputting modulated light corresponding to a predetermined set of linear combinations of the spectral intensity of the light emitted from each pixel; focusing the light outputted from the interferometer on a two dimensional detector array; and processing the output of the detector array to determine the spectral intensity of each pixel thereof; the interferometer being of the translating type in which the optical path difference is varied to modulate the light by translating an element of the interferometer, such that at each instant each detector sees a different point of the scene.
Abstract:
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Abstract:
A method and an apparatus of analyzing an optical image of a scene to determine the spectral intensity of each pixel thereof, comprising: collecting incident light from the scene; passing the light through an interferometer which outputs modulated light corresponding to a predetermined set of linear combinations of the spectral intensity of the light emitted from each pixel; focusing the light outputted from the interferometer on a two dimensional detector array; and processing the output of the detector array to determine the spectral intensity of each pixel thereof; the interferometer being of the low-finesse translating Fabry-Perot type in which the optical path difference is varied to modulate the light by translating an element of the interferometer, such that at each instant each detector sees a different point of the scene and its signal is a linear combination of the spectral content of the light coming from each pixel, and that when the scanner completes one interferometer scan, the scene will have been scanned at all relevant linear combinations of the spectral content.
Abstract:
A method and an apparatus of analyzing an optical image of a scene to determine the spectral intensity of each pixel thereof, comprising: collecting incident light from the scene; passing the light through an interferometer which outputs modulated light corresponding to a predetermined set of linear combinations of the spectral intensity of the light emitted from each pixel; focusing the light outputted from the interferometer on a two dimensional detector array; and processing the output of the detector array to determine the spectral intensity of each pixel thereof; the interferometer being of the low-finesse translating Fabry-Perot type in which the optical path difference is varied to modulate the light by translating an element of the interferometer, such that at each instant each detector sees a different point of the scene.
Abstract:
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
Abstract:
A method and an apparatus for analyzing an optical image of a scene to determine the spectral intensity of each pixel thereof, comprising: collecting and collimating incident light from the scene; passing the light through an interferometer wherein the light is first split into a finite plurality of coherent beams which travel along different optical paths inside the interferometer and then recombine to interfere with each other, the interferometer thereby outputting modulated light corresponding to a predetermined set of linear combinations of the spectral intensity of the light emitted from each pixel; focusing the light outputted from the interferometer on a two dimensional detector array; and processing the output of the detector array to determine the spectral intensity of each pixel thereof; the interferometer being of the translating type in which the optical path difference is varied to modulate the light by translating an element of the interferometer, such that at each instant each detector sees a different point of the scene and its signal is a linear combination of the spectral content of the light coming from each pixel, and that when the scanner completes one interferometer scan, the scene will have been scanned at all relevant linear combinations of the spectral content.