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    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor
    1.
    发明公开
    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor 无效
    用于通过多个宽带滤光器的装置的图像元素分类为根据它们的光谱组的方法和装置

    公开(公告)号：EP0899558A2

    公开(公告)日：1999-03-03

    申请号：EP98306743.0

    申请日：1998-08-24

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： Katzir, Nir ,  Garini, Yuval ,  Wine, David ,  Cabib, Dario

    IPC: G01N21/64

    CPC classification number: G01N21/6428 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6441 ,  G02B21/06

    Abstract: A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

    Abstract translation: 像素分类成像素gemäß的基团及其具有单个荧光团或荧光团的组合关联，包括：（a）提供许多宽带发射滤光片; （B）对于每个像素，记录发射的穿过发射滤波器中的每一个之后作为检索光强度，搜索并每个像素是通过等于宽带发射的数量的维数的矢量表示的 过滤器; 和（c）使用在算法评定每个与每个像素相关联的荧光团的存在，因此，每个像素分类成一组像素gemäß的其与荧光团缔合。 每个荧光团具有一个字符伊辛发射光谱和一个指定发射峰。

    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor
    3.
    发明公开
    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor 审中-公开
    使用多个宽带滤光器和相关联的硬件，根据它们的光谱为像素分类成组的方法

    公开(公告)号：EP1207376A1

    公开(公告)日：2002-05-22

    申请号：EP02001413.0

    申请日：1998-08-24

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： Katzir, Nir ,  Garini, Yuval ,  Wine, David ,  Cabib, Dario

    IPC: G01J3/28

    CPC classification number: G01N21/6428 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6441 ,  G02B21/06

    Abstract: A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

    Abstract translation: 像素分类成雅丁它们与单一荧光团或从荧光团的多个所选择的荧光团的组合关联的像素组的方法，每一个具有表征激发和发射光谱，并指定激发和发射峰的荧光团，该方法包括 （a）提供对宽带激发滤波器（114）和宽谱带发射滤波器（116）的多个的步骤; （B）每个具有通过宽带激发滤波器之一过滤的光的像素的令人兴奋的荧光团，并穿过其配对的发射滤光器之后作为检索记录发射光强度（122）; （C）重复对所有对过滤器的多个步骤（b），搜索并每个像素是通过尺寸的复数，尺寸等于对的多个数目的数目的向量表示的 过滤器; （D）使用算法的评估每个荧光团在每个像素的所述多个的存在，从而每一个像素分类成一组像素gemäß到其用荧光团的单荧光团或组合的关联。

    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor
    4.
    发明公开
    Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardware therefor 无效
    用于通过多个宽带滤光器的装置的图像元素分类为根据它们的光谱组的方法和装置

    公开(公告)号：EP0899558A3

    公开(公告)日：1999-09-29

    申请号：EP98306743.0

    申请日：1998-08-24

    Applicant: Applied Spectral Imaging Ltd.

    Inventor： Katzir, Nir ,  Garini, Yuval ,  Wine, David ,  Cabib, Dario

    IPC: G01N21/64

    CPC classification number: G01N21/6428 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6441 ,  G02B21/06

    Abstract: A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters (114) and wide-band emission filters (116); (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity (122) as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

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