公开(公告)号：WO03027669A2

公开(公告)日：2003-04-03

申请号：PCT/EP0210510

申请日：2002-09-19

Applicant: BASF AG ,  HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24

CPC classification number: G01N33/24

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

Abstract translation: 本发明涉及从土壤样品的核酸提取物生产标准化基因文库的方法，以及其中使用的基因结构和载体。 此外，还包括使用标准化基因库筛选编码来自Bodenprogen的新生物催化剂的基因。

公开(公告)号：AT383575T

公开(公告)日：2008-01-15

申请号：AT02799414

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/24 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88

Abstract: The present invention relates to a method for preparing a normalized gene library from nucleic acid extracts of soil samples and to gene structures and vectors used in said method. The invention further relates to the use of the normalized gene library for the screening of genes coding for novel biocatalysts from the soil samples.

公开(公告)号：CA2461139C

公开(公告)日：2013-08-27

申请号：CA2461139

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: C12N15/63 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

公开(公告)号：DE10146572A1

公开(公告)日：2003-04-10

申请号：DE10146572

申请日：2001-09-21

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24 ,  C12N15/10 ,  C12N15/63

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

公开(公告)号：DE50211528D1

公开(公告)日：2008-02-21

申请号：DE50211528

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/24 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88

Abstract: The present invention relates to a method for preparing a normalized gene library from nucleic acid extracts of soil samples and to gene structures and vectors used in said method. The invention further relates to the use of the normalized gene library for the screening of genes coding for novel biocatalysts from the soil samples.

公开(公告)号：CA2461139A1

公开(公告)日：2003-04-03

申请号：CA2461139

申请日：2002-09-19

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  ZIPPER HUBERT ,  HAUER BERNHARD ,  KAUFFMANN ISABELLE ,  BUTA CHRISTIANE ,  SCHMID ROLF ,  LAEMMLE KATRIN

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24 ,  C12N15/63 ,  C12N15/10 ,  C12N15/66

Abstract: The invention relates to a method for producing a normalized gene library fr om nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.