公开(公告)号：WO03027669A2

公开(公告)日：2003-04-03

申请号：PCT/EP0210510

申请日：2002-09-19

Applicant: BASF AG ,  HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24

CPC classification number: G01N33/24

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

Abstract translation: 本发明涉及从土壤样品的核酸提取物生产标准化基因文库的方法，以及其中使用的基因结构和载体。 此外，还包括使用标准化基因库筛选编码来自Bodenprogen的新生物催化剂的基因。

公开(公告)号：WO2004063359A3

公开(公告)日：2005-01-27

申请号：PCT/EP2004000099

申请日：2004-01-09

Applicant: BASF AG ,  MATUSCHEK MARKUS ,  KLEIN DANIELA ,  HEINEKAMP THORSTEN ,  SCHMIDT ANDRE ,  BRAKHAGE AXEL ,  ACHATZ BRIGITTE

Inventor： MATUSCHEK MARKUS ,  KLEIN DANIELA ,  HEINEKAMP THORSTEN ,  SCHMIDT ANDRE ,  BRAKHAGE AXEL ,  ACHATZ BRIGITTE

IPC: A23K1/16 ,  A23L1/275 ,  A23L1/28 ,  A23L1/30 ,  A61Q90/00 ,  C12N1/15 ,  C12N15/80 ,  C12P23/00 ,  A23J1/00 ,  A23J3/00

CPC classification number: C12N15/80 ,  A23K20/179 ,  A23L5/44 ,  A23L31/00 ,  A23L33/105 ,  C12P23/00

Abstract: The invention relates to a method for producing carotenoids or their precursors using genetically modified organisms of the Blakeslea genus. Said method comprises the following steps (i) transformation of at least one of the cells, (ii) optional homokaryotic conversion of the cells obtained in step (i) to produce cells, in which one or more genetic characteristics of the nucleii are all modified in an identical manner and said modification manifests itself in the cells, (iii) selection and reproduction of the genetically modified cell or cells, (iv) cultivation of the genetically modified cells, (v) preparation of the carotenoids produced by the genetically modified cells or the carotenoid precursor produced by said genetically modified cells. The invention also relates to carotenoids or their precursors produced according to said method and to the use thereof.

Abstract translation: 从而形成在其中通过包括选自（i）获得的细胞（i）的转化细胞中的至少一种，（ii）任选Homokaryotisierung属布拉的转基因生物的装置，用于生产类胡萝卜素或其前体的方法，细胞在芯 或多种遗传特性都是类似的变化和所述遗传修饰的表达，以及（ix）选择和遗传修饰的细胞或细胞的繁殖，（X）培养遗传工程改造的细胞，（XI）提供由所述细胞经遗传修饰 生产的类胡萝卜素或Carotinoidvorstufe由遗传修饰的细胞中产生; 由该方法或它们的前体和它们的使用所产生的类胡萝卜素。

公开(公告)号：DE10341271A1

公开(公告)日：2005-03-31

申请号：DE10341271

申请日：2003-09-08

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  KLEIN DANIELA ,  ACHATZ BRIGITTE ,  HEINEKAMP THORSTEN ,  SCHMIDT ANDRE ,  BRAKHAGE AXEL

IPC: A23K1/16 ,  A23L1/275 ,  A23L1/28 ,  A23L1/30 ,  C12N15/80 ,  C12P23/00 ,  C12N15/09 ,  C07C403/00 ,  C12N1/15

Abstract: Preparing carotenoids (I) or their precursors (Ia) using genetically modified organisms of the genus Blakeslea, is new. Preparing carotenoids (I) or their precursors (Ia) using genetically modified organisms of the genus Blakesleacomprises: (1) transforming at least one cell; (2) optionally homokaryotizing the cells so that cells are produced in which the nuclei are all simultaneously altered in one or more genetic characteristics and these alterations are expressed; (3) selection, multiplication and culture of the modified cell(s); and (4) recovery of (I) and (Ia) from the cells. Independent claims are also included for: (1) producing at least one high-purity (I) and a nutrient, consisting of (I)-producing organisms and at least one (I), by culturing a (I)-producing Blakeslea; and (2) human and animal foods or food supplements produced by the new method.

公开(公告)号：AU2003253323A1

公开(公告)日：2004-02-23

申请号：AU2003253323

申请日：2003-07-25

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  HAUER BERNHARD ,  SCHMID ROLF ,  KAUFFMANN ISABELLE MELANIE ,  BLASCO FRANCESCA ,  SCHMIDT-DANNERT CLAUDIA

IPC: C12N9/02 ,  C12P23/00

Abstract: The invention relates to a process for the biotransformation of carotenoids using enzymes having cytochrome P450 monooxygenase activity; in particular monooxygenases from thermophilic bacteria, in particular of the genus Thermus sp.

公开(公告)号：DE10223057A1

公开(公告)日：2003-12-11

申请号：DE10223057

申请日：2002-05-24

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  HAUER BERNHARD

IPC: C12N15/09 ,  C12N15/10 ,  C12P19/34 ,  C07H21/00 ,  C12N15/11

Abstract: The invention relates to methods for creating polynucleotide molecules with modified characteristics, said methods running through a cycle comprising the following steps:(a) provision of a double-stranded polynucleotide molecule or a population of double-stranded polynucleotide molecules, the individual polynucleotides of said population having at least one homologous sequence part and at least one heterologous sequence part,(b) creation of single-strand breaks in the double-stranded polynucleotide molecules, (c) nucleolytic degradation in the 5'} 3' direction starting from the single-strand breaks with simultaneous new synthesis in the 5' } 3' direction and shifting of the single-strand breaks in the direction of the 3' end, (d) production of single-stranded polynucleotide molecules, (e) production of partially double-stranded polynucleotide molecules of the single-stranded polynucleotide molecules provided in step (d), (f) template-directed nucleic acid synthesis starting from the partially double-stranded polynucleotide molecules produced in step (e), whereby steps (b) and (c) can be carried out in succession or simultaneously.

公开(公告)号：DE50211528D1

公开(公告)日：2008-02-21

申请号：DE50211528

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/24 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88

Abstract: The present invention relates to a method for preparing a normalized gene library from nucleic acid extracts of soil samples and to gene structures and vectors used in said method. The invention further relates to the use of the normalized gene library for the screening of genes coding for novel biocatalysts from the soil samples.

公开(公告)号：DE102005057178A1

公开(公告)日：2007-07-19

申请号：DE102005057178

申请日：2005-11-30

Applicant: BASF AG

Inventor： KLATT MARTIN JOCHEN ,  HASSELWANDER OLIVER ,  MATUSCHEK MARKUS ,  HAUNERT FRANK

IPC: A61K31/385 ,  A61P3/00

公开(公告)号：AU2003240683A1

公开(公告)日：2003-12-12

申请号：AU2003240683

申请日：2003-05-21

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  HAUER BERNHARD

IPC: C12N15/09 ,  C12N15/10 ,  C12P19/34

Abstract: The invention relates to methods for creating polynucleotide molecules with modified characteristics, said methods running through a cycle comprising the following steps:(a) provision of a double-stranded polynucleotide molecule or a population of double-stranded polynucleotide molecules, the individual polynucleotides of said population having at least one homologous sequence part and at least one heterologous sequence part,(b) creation of single-strand breaks in the double-stranded polynucleotide molecules, (c) nucleolytic degradation in the 5'} 3' direction starting from the single-strand breaks with simultaneous new synthesis in the 5' } 3' direction and shifting of the single-strand breaks in the direction of the 3' end, (d) production of single-stranded polynucleotide molecules, (e) production of partially double-stranded polynucleotide molecules of the single-stranded polynucleotide molecules provided in step (d), (f) template-directed nucleic acid synthesis starting from the partially double-stranded polynucleotide molecules produced in step (e), whereby steps (b) and (c) can be carried out in succession or simultaneously.

公开(公告)号：CA2461139A1

公开(公告)日：2003-04-03

申请号：CA2461139

申请日：2002-09-19

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  ZIPPER HUBERT ,  HAUER BERNHARD ,  KAUFFMANN ISABELLE ,  BUTA CHRISTIANE ,  SCHMID ROLF ,  LAEMMLE KATRIN

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24 ,  C12N15/63 ,  C12N15/10 ,  C12N15/66

Abstract: The invention relates to a method for producing a normalized gene library fr om nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

公开(公告)号：CA2461139C

公开(公告)日：2013-08-27

申请号：CA2461139

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: C12N15/63 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12N15/10 ,  C12N15/66 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.