公开(公告)号：WO0107574A2

公开(公告)日：2001-02-01

申请号：PCT/EP0007252

申请日：2000-07-27

Applicant: BASF AG ,  HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING SHAN

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12M1/40 ,  C12N1/21 ,  C12N9/02 ,  C12N15/09 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/11 ,  C12R1/19 ,  C12N9/00

CPC classification number: C12P7/6409 ,  C12N9/0071 ,  C12N9/0077 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P17/10 ,  C12P17/165 ,  C12Q1/26 ,  G01N2333/90245

Abstract: The invention relates to modified cytochrome P450 monooxygenases comprising a modified substrate profile, to nucleic acid sequences that code therefor, to expression constructs and vectors, and to recombinant microorganisms which contain these vectors. The invention also relates to methods for microbiologically producing terminally or subterminally hydroxylated aliphatic carboxylic acids.

Abstract translation: 本发明涉及具有改变的底物轮廓，编码核酸序列，表达构建体和载体，含有用于微生物生产终端或近hydroylierter脂族羧酸，这些载体和方法重组微生物修饰细胞色素P450单加氧酶。

公开(公告)号：WO03027669A2

公开(公告)日：2003-04-03

申请号：PCT/EP0210510

申请日：2002-09-19

Applicant: BASF AG ,  HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24

CPC classification number: G01N33/24

Abstract: The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.

Abstract translation: 本发明涉及从土壤样品的核酸提取物生产标准化基因文库的方法，以及其中使用的基因结构和载体。 此外，还包括使用标准化基因库筛选编码来自Bodenprogen的新生物催化剂的基因。

公开(公告)号：WO0233057A2

公开(公告)日：2002-04-25

申请号：PCT/EP0111958

申请日：2001-10-16

Applicant: BASF AG ,  HAUER BERNHARD ,  SCHMID ROLF ,  MERKL RAINER ,  BLASCO FRANCESCA

Inventor： HAUER BERNHARD ,  SCHMID ROLF ,  MERKL RAINER ,  BLASCO FRANCESCA

IPC: C12N15/09 ,  C12M1/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/53 ,  C12P1/04 ,  C12P7/00 ,  C12R1/01 ,  C12N9/00

CPC classification number: C12N9/0077 ,  C12P1/04

Abstract: The invention relates to novel cytochrome P450 monooxygenases consisting of thermophilic bacteria, especially the species Thermus sp., nucleotide sequences coding for the same, the recombinant production of said monooxygenases and the use thereof for the microbiological oxidation of organic compounds.

Abstract translation: 本发明涉及来自嗜热菌的新的细胞色素P450单加氧酶，尤其是杆菌属栖热菌种，核苷酸序列编码为此，重组生产这些单加氧酶的和它们用于有机化合物的微生物氧化的用途。

公开(公告)号：HU229450B1

公开(公告)日：2013-12-30

申请号：HU0202074

申请日：2000-07-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN ,  LUTZ-WAHL SABINE ,  APPEL DANIEL

IPC: C12N15/09 ,  C12N15/53 ,  C12M1/40 ,  C12N1/21 ,  C12N9/02 ,  C12N15/70 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/19

公开(公告)号：DE50211528D1

公开(公告)日：2008-02-21

申请号：DE50211528

申请日：2002-09-19

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  MATUSCHEK MARKUS ,  SCHMID ROLF ,  BUTA CHRISTIANE ,  KAUFFMANN ISABELLE ,  LAEMMLE KATRIN ,  ZIPPER HUBERT

IPC: G01N33/24 ,  G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88

Abstract: The present invention relates to a method for preparing a normalized gene library from nucleic acid extracts of soil samples and to gene structures and vectors used in said method. The invention further relates to the use of the normalized gene library for the screening of genes coding for novel biocatalysts from the soil samples.

公开(公告)号：DE102006016023A1

公开(公告)日：2007-10-11

申请号：DE102006016023

申请日：2006-04-05

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  HAERING DIETMAR ,  BRANNEBY CECILIA ,  SCHMID ROLF ,  RUSNAK MONIKA ,  LIU DANNI

IPC: C12N15/55 ,  C12N9/20 ,  C12N15/70 ,  C12Q1/44

Abstract: The present invention relates to nucleic acids that code for triacylglycerol lipases, vectors comprising said nucleic acids, host cells comprising said nucleic acids or vectors, methods for the expression of triacylglycerol lipases in prokaryotes, methods for the detection and for the production of triacylglycerol lipases, triacylglycerol lipases obtainable thereby, and the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for said methods.

公开(公告)号：AT348150T

公开(公告)日：2007-01-15

申请号：AT04739133

申请日：2004-05-05

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  HABICHER TILO ,  SCHMID ROLF ,  MAURER STEFFEN CHRISTIAN ,  URLACHER VLADA BENIAMINOVNA ,  SCHULZE HOLGER ,  HUBER NORBERT ,  BACHMANN TILL T

IPC: C12N9/02 ,  C12Q1/26

Abstract: A process for preparing a hydroxylation catalyst by i) embedding a cystochrome P450 monooxygenase in a sol-gel matrix, ii) embedding an enzymatic NADPH-regenerating system in a sol-gel matrix, and combining the two components i) and ii) unless they were already mixed together before the embedding.

公开(公告)号：UA76701C2

公开(公告)日：2006-09-15

申请号：UA200221606

申请日：2000-07-27

Applicant: BASF AG

Inventor： FISCHER MARKUS ,  SCHMID ROLF

IPC: C12N15/09 ,  C12M1/40 ,  C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/19

Abstract: Винахідналежитьдоспособумікробіологічногоокиснення N-, O- або S- гетероциклічниходно- абобагатоядернихароматичнихсполук, способумікробіологічногоокисненнязаміщениходно- абобагатоядернихароматичнихвуглеводнів, нерозгалуженихаборозгалуженихалканіві алкенів, способумікробіологічноговиробництваіндигоі/абоіндирубінузадопомогоюмутованоїцитохромР450-монооксигенази.

公开(公告)号：ZA200303761B

公开(公告)日：2004-07-05

申请号：ZA200303761

申请日：2003-05-15

Applicant: BASF AG

Inventor： BLASCO FRANCESCA ,  HAUER BERNHARD ,  MERKL RAINER ,  SCHMID ROLF

IPC: C12M1/40 ,  C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/53 ,  C12P1/04 ,  C12P7/00 ,  C12R1/01 ,  C12N

Abstract: The invention relates to novel cytochrome P450 monooxygenases from thermophilic bacteria, in particular the genus Thermus sp., to nucleotide sequences encoding them, to the recombinant production of these monooxygenases and to their use for the microbiological oxidation of organic compounds.

公开(公告)号：CA2461139A1

公开(公告)日：2003-04-03

申请号：CA2461139

申请日：2002-09-19

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  ZIPPER HUBERT ,  HAUER BERNHARD ,  KAUFFMANN ISABELLE ,  BUTA CHRISTIANE ,  SCHMID ROLF ,  LAEMMLE KATRIN

IPC: G01N33/50 ,  B01J20/281 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/78 ,  G01N30/26 ,  G01N30/88 ,  G01N33/24 ,  C12N15/63 ,  C12N15/10 ,  C12N15/66

Abstract: The invention relates to a method for producing a normalized gene library fr om nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.