公开(公告)号：DE10011723A1

公开(公告)日：2001-09-13

申请号：DE10011723

申请日：2000-03-10

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEIS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12N9/02 ,  C12N15/53 ,  C12P7/42 ,  C12P7/64 ,  C12Q1/26 ,  C12N15/70

Abstract: An electron donor system (M1) that transfers electrons to an enzyme with redox properties comprising an inorganic, not electrode-bound, electron sink and a mediator which enable the electron transfer is new. Independent claims are also included for: (1) transferring (M2) oxygen to a hydrocarbon containing hydrogen donor molecule, where the hydrogen donor molecule is in a reaction medium comprising the oxygen transferring enzyme and (M1) in the presence of oxygen and incubating under suitable reaction conditions; (2) the enzymatic production (M3) of terminally or subterminally hydroxylated (position w-1 to w-4) fatty acids comprising: (i) mixing a hydroxylatable fatty acid or fatty acid derivative in the presence of (M1) and cytochrome P450 monooxygenase; and (ii) isolating the hydroxylated product; (3) a bioreactor useful for the production of w-hydroxylated fatty acids as described in (2); and (4) detecting (M4) fatty acid-monooxygenases comprising: (i) contacting the analyte with a w-hydroxylatable fatty acid or a derivative comprising a terminal chromophore or fluorescent label in (M1); and (ii) qualitatively or quantitatively detecting the signal.