公开(公告)号：DE4403666A1

公开(公告)日：1995-08-10

申请号：DE4403666

申请日：1994-02-07

Applicant: BASF AG

Inventor： HOEGER THOMAS DR ,  ULTSCH ANDREAS DR ,  BACH ALFRED DR ,  STERRER SYLVIA DR ,  LEMAIRE HANS-GEORG DR

IPC: G01N33/567 ,  C07K14/705 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/68 ,  C12Q1/00 ,  C07H21/04

Abstract: The invention pertains to new glutamate receptor subunits and DNA sequences coding for them and to methods for producing DNA sequences and receptors. The invention also pertains to methods for identifying functional ligands for these receptors.

公开(公告)号：DE4410882A1

公开(公告)日：1995-10-05

申请号：DE4410882

申请日：1994-03-29

Applicant: BASF AG

Inventor： STERRER SYLVIA DR ,  ULTSCH ANDREAS DR ,  HOEGER THOMAS DR ,  LEMAIRE HANS-GEORG DR ,  BACH ALFRED DR

IPC: G01N33/566 ,  C07K14/705 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/91 ,  C12N15/79 ,  C12Q1/00 ,  G01N33/50

Abstract: The invention concerns a method for the preparation of eucaryotic cell lines for the permanently ectopic expression of glutamate receptors, the cell lines being prepared by the transformation of cells containing nucleic acids coding for glutamate receptors. The method is characterized in that, during the establishment of the cell lines, at least one of the following conditions is satisfied for the culture: a) the transformed cells are cultured in a culture medium containing a glutamate precursor; b) the transformed cells are cultured in the presence of a glutamate-receptor antagonist; c) the transformed cells are cultured in a first phase under conditions in which glutamate-receptor expression is repressed and in a second phase under conditions in which the repression is discontinued. The invention also concerns the cell lines thus obtained and their use.