Abstract:
Die vorliegende Erfindung betrifft neuartige Verfahren zur biokatalytischen Herstellung von Nitrilen aus Oximen unter Verwendung von Oxim-Dehydratasen sowie neuartige Mutanten mit Oxim-Dehydratase-Aktivität und deren Anwendung in einem Verfahren zur biokatalytischen Herstellung von Nitrilen, wie insbesondere zur Herstellung von Citralnitril, Neralnitril, Geranialnitril oder Citronellylnitril aus Citraloxim, Neraloxim, Geranialoxim beziehungsweise Citronellaloxim; sowie dafür einsetzbare Oxim-Dehydratasen, Nukleotidsequenzen dafür sowie diese umfassende Expressionkonstrukte oder Mikroorganismen.
Abstract:
The invention relates to the use of an enzyme preparation which catalyzes the degradation of formaldehyde for reducing the formaldehyde content in a formaldehyde-containing formulation. In a preferred embodiment, the enzyme preparation contains a formaldehyde dismutase from a Pseudomonas putida strain. Further, the invention refers to a process for reducing the formal dehyde content in cross-linking agents for textile finishing or in polymer dispersions used, e.g. in construction chemistry. Further the invention relates to the use of an enzyme preparation which catalyzes the degradation of aldehydes for reducing the formaldehyde content in an aldehyde-containing formulation. Furthermore, the invention relates to a novel variant of the formaldehyde dismutase from Pseudomonas putida.
Abstract:
An indicator for temperature monitoring a chilled or frozen product, which indicator comprises an enzyme which catalyzes the conversion of a polysaccharide to a monosaccharide, and enzyme which catalyzes the oxidation of the monosaccharide formed, and a mixture which is suitable for detecting the enzyme activities and which comprises a color indicator. In addition, a method for producing the indicator, a method for temperature monitoring and a package provided with the indicator thereof.
Abstract:
A process for preparing sebacic acid by reacting in a first step (i) linoleic acid with water catalyzed by an oleate hydratase to form10-hydroxy-12-octadecenoic acid, in a second step (ii) pyrolysing the 10-hydroxy-12-octadecenoic acid to 1-octene and 10-oxo-decanoic acid and in a third step (iii) oxidizing the 10-oxo-decanoic acid to sebacic acid.
Abstract:
The present invention relates to methods of a new type for biocatalytically producing nitriles from oximes using oxime dehydratases and to mutations of a new type having oxime dehydratase activity and use thereof in a method for biocatalytically producing nitriles, such as in particular for producing citral nitrile, neral nitrile, geranial nitrile, or citronellyl nitrile from citral oxime, neral oxime, geranial oxime, or citronellal oxime; and to oxime dehydratases that can be used therefor, nucleotide sequences therefor, and expression constructs or microorganisms comprising said nucleotide sequences.
Abstract:
A process for preparing sebacic acid by reacting in a first step (i) linoleic acid with water catalyzed by an oleate hydratase to form10- hydroxy-12-octadecenoic acid, in a second step (ii) pyrolysing the 10-hydroxy-12-octadecenoic acid to 1-octene and 10-oxo-decanoic acid and in a third step(iii) oxidizing the 10-oxo-decanoic acid to sebacic acid.
Abstract:
The invention relates to the use of an enzyme preparation which catalyzes the degradation of formaldehyde for reducing the formaldehyde content in a formaldehyde-containing formulation. In a preferred embodiment, the enzyme preparation contains a formaldehyde dismutase from a Pseudomonas putida strain. Further, the invention refers to a process for reducing the formal dehyde content in cross-linking agents for textile finishing or in polymer dispersions used, e.g. in construction chemistry. Further the invention relates to the use of an enzyme preparation which catalyzes the degradation of aldehydes for reducing the formaldehyde content in an aldehyde-containing formulation. Furthermore, the invention relates to a novel variant of the formaldehyde dismutase from Pseudomonas putida.
Abstract:
A process for preparing sebacic acid by reacting in a first step (i) linoleic acid with water catalyzed by an oleate hydratase to form10- hydroxy-12-octadecenoic acid, in a second step (ii) pyrolysing the 10-hydroxy-12-octadecenoic acid to 1-octene and 10-oxo-decanoic acid and in a third step(iii) oxidizing the 10-oxo-decanoic acid to sebacic acid.