公开(公告)号：US3761226A

公开(公告)日：1973-09-25

申请号：US3761226D

申请日：1972-03-24

Applicant: BAXTER LABORATORIES INC

Inventor： LOUDERBACK A ,  YOUHNE Y ,  FONTANA A

IPC: G01N33/72 ,  G01N21/06 ,  G01N33/16

CPC classification number: G01N33/52 ,  G01N33/5091 ,  G01N33/721

Abstract: A DIAGNOSTIC TEST AND REAGENT FOR THE RAPID IDENTIFICATION OF SICKLE-CELL ANEMIA AND SICKLE-CELL TRAIT COMPRISING REACTION OF UNCLOTTED BLOOD WITH AN ADMIXTURE OF (NH4)2SO4/K2HPO4 BUFFER, PH 7-7.5, SAPONIN AND SODIUM DITHIONITE AND OBSERVATION OF THE TURBIDOMETRICCOLORMETRIC CHARACTERISTICS.

公开(公告)号：BE746068A

公开(公告)日：1970-07-31

申请号：BE746068D

申请日：1970-02-17

Applicant: BAXTER LABORATORIES INC

Inventor： LOUDERBACK A L ,  YOUHNE Y

IPC: G01N33/49 ,  G01N15/12 ,  G01N33/543 ,  G01N33/96 ,  A61B

公开(公告)号：ZA731900B

公开(公告)日：1973-12-19

申请号：ZA731900

申请日：1973-03-19

Applicant: BAXTER LABORATORIES INC

Inventor： LOUDERBACK A ,  YOUHNE Y ,  FONTANA A

IPC: G01N33/72 ,  A61K

Abstract: 1377346 Diagnostic agent for detecting sicklecell disease BAXTER LABORATORIES Inc 26 March 1973 [24 March 1972] 14443/73 Heading G1B A reagent kit for use in testing blood for sicklecell disease and for distinguishing between sicklecell anemia and sickle-cell trait comprises (a) an aqueous buffer solution having a pH of from 7 to 7À5 and containing, per 100 ml of solution, from 21 to 35 grams of (NH 4 ) 2 SO 4 and from 0À5 to 2À0 grams of K 2 HPO 4 , (b) a hemolyzing agent, for example saponin, and (c) a reducing agent, for example sodium dithionite. The hemolyzing agent and the reducing agent may be in powdered form. Reagent solution prepared by mixing the contents of such kit may contain, per 100 ml of solution, from 0À01 to 2 grams of hemolyzing agent and from 0À01 to 2 grams of reducing agent. A sample of unclotted blood is mixed with a predetermined amount of reagent solution, the mixture being observed visually both before and after centrifuging. A translucent solution before centrifuging indicates normal (AA) hemoglobin whereas a turbid solution indicates either sickle-cell trait (AS) hemoglobin or sickle-cell anemia (SS) hemoglobin; a small amount of white precipitate after centrifuging indicates normal hemoglobin whereas a pink solution with red precipitate indicates sickle-cell hemoglobin and a yellow solution with red precipitate indicates sickle-cell anemia hemoglobin. The buffer solution additionally may contain a redox dye, for example methylene blue or basic fuchsin, for indicating decomposition of sodium dithionite reducing agent.

公开(公告)号：SE377966B

公开(公告)日：1975-08-04

申请号：SE281370

申请日：1970-03-03

Applicant: BAXTER LABORATORIES INC

Inventor： LOUDERBACK A L ,  YOUHNE Y

IPC: G01N33/49 ,  G01N15/12 ,  G01N33/543 ,  G01N33/96

公开(公告)号：SE365614B

公开(公告)日：1974-03-25

申请号：SE64471

申请日：1971-01-20

Applicant: BAXTER LABORATORIES INC

Inventor： YOUHNE Y ,  LOUDERBACK A

IPC: G01N20060101 ,  G01N33/50

公开(公告)号：ZA7301900B

公开(公告)日：1973-12-19

申请号：ZA7301900

申请日：1973-03-19

Applicant: BAXTER LABORATORIES INC

Inventor： LOUDERBACK A ,  YOUHNE Y ,  FONTANA A

IPC: G01N33/72 ,  A61K

CPC classification number: G01N33/52 ,  G01N33/5091 ,  G01N33/721

公开(公告)号：CA932632A

公开(公告)日：1973-08-28

申请号：CA103191

申请日：1971-01-20

Applicant: BAXTER LABORATORIES INC

Inventor： YOUHNE Y ,  LOUDERBACK A

IPC: G01N20060101

公开(公告)号：BE761686A

公开(公告)日：1971-07-01

申请号：BE761686

申请日：1971-01-18

Applicant: BAXTER LABORATORIES INC

Inventor： YOUHNE Y ,  LOUDERBACK A L

IPC: C12Q1/48 ,  C12Q1/61 ,  G01N