公开(公告)号：CA2160799A1

公开(公告)日：1994-10-27

申请号：CA2160799

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12P19/34

Abstract: Disclosed herein, inter alia, are Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：WO9424312A3

公开(公告)日：1994-12-08

申请号：PCT/US9404275

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C07H21/00 ,  C12N15/1034 ,  C12Q1/6853 ,  C12Q1/6862

Abstract: Disclosed herein, inter alia, are Inverse Linkage Oligonucleotides ('ILO') useful in enzymatic process. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：AU6707794A

公开(公告)日：1994-11-08

申请号：AU6707794

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12P19/34

Abstract: Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：AU6543696A

公开(公告)日：1997-02-10

申请号：AU6543696

申请日：1996-07-10

Applicant: BECKMAN INSTRUMENTS INC

Inventor： PENTONEY STEPHEN L JR ,  KONRAD KENNETH D

IPC: G01N27/447

Abstract: An electrophoretic system employing a capillary with at least one end having an electrically conductive coating or layer directly thereon. In order to introduce a plug of sample from a small amount of the sample in a vial, the conductive tip is submersed into the small amount of sample and an electric field is applied at the end by applying the high voltage to the tip in order to electrokinetically inject a plug of the sample. The conductive tip may also be used as a terminal for completing the circuit for applying the high voltage across the capillary column for electrophoresis. The separated components may be collected on a surface or small amount of buffer to reduce sample dilution or mixing of one sample component with a different sample component from the electrophoretic process.