公开(公告)号：WO9509176A1

公开(公告)日：1995-04-06

申请号：PCT/US9309294

申请日：1993-09-28

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  MATSON ROBERT S ,  RAMPAL JANG B

IPC: C07H21/00 ,  C08F8/00 ,  C08F8/32 ,  C12Q1/68 ,  C07K1/04 ,  C08F8/30 ,  C08J3/28 ,  G01N33/68

CPC classification number: C07H21/00 ,  C08F8/30 ,  C08F8/32 ,  C12Q1/6827 ,  C12Q1/6834 ,  C08F110/06

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

Abstract translation: 本文公开了可用于生物聚合物合成的表面活化的有机聚合物。 最优选地，胺化聚丙烯用于合成寡核苷酸，并且这些装置最优选用于患者样品的遗传分析。

公开(公告)号：WO9424312A3

公开(公告)日：1994-12-08

申请号：PCT/US9404275

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C07H21/00 ,  C12N15/1034 ,  C12Q1/6853 ,  C12Q1/6862

Abstract: Disclosed herein, inter alia, are Inverse Linkage Oligonucleotides ('ILO') useful in enzymatic process. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：WO9511912A3

公开(公告)日：1995-06-08

申请号：PCT/US9412437

申请日：1994-10-28

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  MATSON ROBERT S ,  RAMPAL JANG B

IPC: G01N33/53 ,  A61K38/00 ,  A61K39/44 ,  B01J19/00 ,  C07H21/00 ,  C07H21/04 ,  C07K1/04 ,  C07K7/06 ,  C07K7/08 ,  C08B37/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  G01N33/543 ,  G01N33/545 ,  G01N33/566 ,  G01N37/00

CPC classification number: C07H21/00 ,  B01J19/0046 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  C07K1/042 ,  C12Q1/6834 ,  C12Q1/6869 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  G01N33/54353 ,  Y10T436/143333

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated biaxially oriented polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

公开(公告)号：AU1530497A

公开(公告)日：1997-08-11

申请号：AU1530497

申请日：1997-01-08

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  NEAL JACK D MC ,  HELPHREY DAVID B

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/02 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N21/27 ,  G01N21/47 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N37/00

Abstract: An analytical biochemistry system featuring a substrate with reactants immobilized thereon at fixed, known locations, a holder supporting the substrate and a manipulator for transporting the holder to a fixed sample and to an inspection station. The reactants are binding agents for a target biomolecule in a sample which forms a bound substance having a detectable characteristic. The holder may be a standard pipettor, optionally carried by a robot arm or hand as the manipulator to contact the sample for detection of the presence of target biomolecules within the sample. In one embodiment, the holder is a pipette tip within which the substrate is housed, or it may be a pipette adapter which bears the substrate and fits within the sample wells of a standard microtiter plate. After appropriate incubation, the substrate and holder may be moved from contact with the sample, and the substrate may be exposed to any necessary development steps before being moved by the manipulator to a location for probing, such as by a beam. Probing of the substrate is performed to identify binding or complexing of target biomolecules of the sample with the reactants immobilized on the substrate, and may be accomplished via fluorescence detection, light scattering, autoradiography, or some other detection method.

公开(公告)号：AU734126C

公开(公告)日：2002-01-03

申请号：AU1530497

申请日：1997-01-08

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  NEAL JACK D MC ,  HELPHREY DAVID B

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/02 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N21/27 ,  G01N21/47 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N37/00

Abstract: An analytical biochemistry system featuring a substrate with reactants immobilized thereon at fixed, known locations, a holder supporting the substrate and a manipulator for transporting the holder to a fixed sample and to an inspection station. The reactants are binding agents for a target biomolecule in a sample which forms a bound substance having a detectable characteristic. The holder may be a standard pipettor, optionally carried by a robot arm or hand as the manipulator to contact the sample for detection of the presence of target biomolecules within the sample. In one embodiment, the holder is a pipette tip within which the substrate is housed, or it may be a pipette adapter which bears the substrate and fits within the sample wells of a standard microtiter plate. After appropriate incubation, the substrate and holder may be moved from contact with the sample, and the substrate may be exposed to any necessary development steps before being moved by the manipulator to a location for probing, such as by a beam. Probing of the substrate is performed to identify binding or complexing of target biomolecules of the sample with the reactants immobilized on the substrate, and may be accomplished via fluorescence detection, light scattering, autoradiography, or some other detection method.

公开(公告)号：AU734126B2

公开(公告)日：2001-06-07

申请号：AU1530497

申请日：1997-01-08

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  NEAL JACK D MC ,  HELPHREY DAVID B

IPC: G01N21/64 ,  B01J19/00 ,  B01L3/02 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N21/27 ,  G01N21/47 ,  G01N21/78 ,  G01N33/483 ,  G01N33/53 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N37/00

Abstract: An analytical biochemistry system featuring a substrate with reactants immobilized thereon at fixed, known locations, a holder supporting the substrate and a manipulator for transporting the holder to a fixed sample and to an inspection station. The reactants are binding agents for a target biomolecule in a sample which forms a bound substance having a detectable characteristic. The holder may be a standard pipettor, optionally carried by a robot arm or hand as the manipulator to contact the sample for detection of the presence of target biomolecules within the sample. In one embodiment, the holder is a pipette tip within which the substrate is housed, or it may be a pipette adapter which bears the substrate and fits within the sample wells of a standard microtiter plate. After appropriate incubation, the substrate and holder may be moved from contact with the sample, and the substrate may be exposed to any necessary development steps before being moved by the manipulator to a location for probing, such as by a beam. Probing of the substrate is performed to identify binding or complexing of target biomolecules of the sample with the reactants immobilized on the substrate, and may be accomplished via fluorescence detection, light scattering, autoradiography, or some other detection method.

公开(公告)号：AU671688B2

公开(公告)日：1996-09-05

申请号：AU5294493

申请日：1993-09-28

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  MATSON ROBERT S ,  RAMPAL JANG B

IPC: C07H21/00 ,  C08F8/00 ,  C08F8/32 ,  C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6834 ,  C07K1/04 ,  C08F8/30 ,  C07H3/00 ,  C08J7/12

Abstract: Disclosed herein are surface activated, organic polymers useful for biopolymer synthesis. Most preferably, aminated polypropylene is used for the synthesis of oligonucleotides thereto, and these devices are most preferably utilized for genetic analysis of patient samples.

公开(公告)号：AU2360195A

公开(公告)日：1995-11-29

申请号：AU2360195

申请日：1995-04-24

Applicant: BECKMAN INSTRUMENTS INC ,  CHARLES THOMAS CASKEY

Inventor： CASKEY CHARLES THOMAS ,  MATSON ROBERT S ,  COASSIN PETER J ,  RAMPAL JANG B

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization to a reverse dot blot array comprising strings of such repeats complementary to those found in particular nucleic acid targets (e.g., analyte PCR product). An addressable library (i.e., an indexed set) of complementary repeats is synthesized on a suitable support. Preferably, the support comprises a low fluorescent background support, thereby facilitating the use of non-radioisotopic modes of detection (such as fluorescence or chemiluminescence); particularly suitable in this regard is an aminated polypropylene support or similar material. Preferred arrays permit screening of DNA and RNA samples for complete sets of particular types of nucleotide repeat sequences (e.g., all nucleotide doublet or triplet repeats).

公开(公告)号：CA2160799A1

公开(公告)日：1994-10-27

申请号：CA2160799

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12P19/34

Abstract: Disclosed herein, inter alia, are Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

公开(公告)号：AU6707794A

公开(公告)日：1994-11-08

申请号：AU6707794

申请日：1994-04-19

Applicant: BECKMAN INSTRUMENTS INC

Inventor： COASSIN PETER J ,  KONRAD KENNETH D ,  RAMPAL JANG B ,  COOK RONALD M

IPC: C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6862 ,  C12P19/34

Abstract: Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.