公开(公告)号：AU2014238108B2

公开(公告)日：2018-02-15

申请号：AU2014238108

申请日：2014-02-26

Applicant: BIO RAD LABORATORIES

Inventor： DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

IPC: C12Q1/68 ,  C07H21/04

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

公开(公告)号：AU2014238108A1

公开(公告)日：2015-10-08

申请号：AU2014238108

申请日：2014-02-26

Applicant: BIO RAD LABORATORIES ,  MAAR DIANNA ,  DO DUC ,  LITTERST CLAUDIA

Inventor： DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

IPC: C07H21/04

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

公开(公告)号：SG11201507617WA

公开(公告)日：2015-10-29

申请号：SG11201507617W

申请日：2014-02-26

Applicant: BIO RAD LABORATORIES ,  DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

Inventor： DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

IPC: C07H21/04

公开(公告)号：EP2970367A4

公开(公告)日：2016-08-31

申请号：EP14769970

申请日：2014-02-26

Applicant: BIO RAD LABORATORIES ,  DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

Inventor： DO DUC ,  LITTERST CLAUDIA ,  MAAR DIANNA

IPC: C07H21/04 ,  C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2565/119 ,  C12Q2527/143 ,  C12Q2545/101 ,  C12Q2563/159 ,  C12Q2563/173

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

Abstract translation: 数字测定系统，包括方法，装置和组合物，用于测定含有目标扩增通用报告物的一组分区中的一个或多个靶。 数字测定通常依赖于检测样品中分析物的单个拷贝的存在或活性的能力。 在示例性的数字测定中，将样品分成通常具有相等体积的一组分隔体，每个分隔物平均含有小于约一份的分析物。

公开(公告)号：EP2888375A4

公开(公告)日：2016-03-30

申请号：EP13831166

申请日：2013-08-22

Applicant: BIO RAD LABORATORIES

Inventor： LITTERST CLAUDIA ,  SO AUSTIN P ,  DO DUC

IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04

CPC classification number: G01N21/6486 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2525/204 ,  C12Q2563/159

Abstract: Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.