Digital assays with a generic reporter

    公开(公告)号:AU2014238108B2

    公开(公告)日:2018-02-15

    申请号:AU2014238108

    申请日:2014-02-26

    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

    Digital assays with a generic reporter

    公开(公告)号:AU2014238108A1

    公开(公告)日:2015-10-08

    申请号:AU2014238108

    申请日:2014-02-26

    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

    DIGITAL ASSAYS WITH A GENERIC REPORTER
    4.
    发明公开
    DIGITAL ASSAYS WITH A GENERIC REPORTER 有权
    数字测定麻省理工学院通讯员

    公开(公告)号:EP2970367A4

    公开(公告)日:2016-08-31

    申请号:EP14769970

    申请日:2014-02-26

    Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification. Digital assays generally rely on the ability to detect the presence or activity of individual copies of an analyte in a sample. In an exemplary digital assay, a sample is separated into a set of partitions, generally of equal volume, with each containing, on average, less than about one copy of the analyte.

    Abstract translation: 数字测定系统,包括方法,装置和组合物,用于测定含有目标扩增通用报告物的一组分区中的一个或多个靶。 数字测定通常依赖于检测样品中分析物的单个拷贝的存在或活性的能力。 在示例性的数字测定中,将样品分成通常具有相等体积的一组分隔体,每个分隔物平均含有小于约一份的分析物。

    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
    5.
    发明公开
    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS 有权
    AMPLIFIKATIONSREPORTER MIT BASENPAARUNGSOLIGOMEREN

    公开(公告)号:EP3033445A4

    公开(公告)日:2017-03-22

    申请号:EP14836787

    申请日:2014-08-12

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    Abstract translation: 用扩增报道分子进行扩增测定的系统,包括能够在报道分子的解链温度以下彼此碱基配对的第一寡聚物和第二寡聚物。 报道分子可能具有可检测的光致发光,其通过第一和第二寡聚体彼此碱基配对而受到影响,例如减少。 诸如核酸靶序列之类的靶可以在熔解温度以上的至少一个体积(诸如多个分区)中被扩增,并且报道子的光致发光可以从低于解链温度的至少一个体积中被检测到 。 可以基于检测到的光致发光来确定目标的属性,诸如目标的浓度。

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