公开(公告)号：WO2012112970A3

公开(公告)日：2013-11-14

申请号：PCT/US2012025760

申请日：2012-02-18

Applicant: BIO RAD LABORATORIES ,  HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY ,  HODGES SHAWN PAUL ,  HEREDIA NICHOLAS J ,  MELLEN JEFFREY CLARK ,  TROUP CAMILLE BODLEY ,  WYATT PAUL

Inventor： HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY ,  HODGES SHAWN PAUL ,  HEREDIA NICHOLAS J ,  MELLEN JEFFREY CLARK ,  TROUP CAMILLE BODLEY ,  WYATT PAUL

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6883 ,  C12Q2521/331 ,  C12Q2523/125 ,  C12Q2600/154

Abstract: Methods and compositions for detecting and quantifying polynucleotide in a sample. Specifically, methylated DNA is quantified to determine the load ofpolynucleotides in a sample. Non-invasive methods for determining the load of fetal polynucleotides in maternal plasma. Methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. Methods for detecting differences in copy number of a target polynucleotide, specifically, for diagnosis of fetal genetic abnormalities, when the starting sample is maternal blood, serum, or plasma.

Abstract translation: 用于检测和定量样品中多核苷酸的方法和组合物。 具体地，定量甲基化的DNA以确定样品中多核苷酸的负载。 用于确定母体血浆中胎儿多核苷酸负荷的非侵入性方法。 用于检测细胞过程如细胞活力，生长速率和感染率的方法和组合物。 用于检测目标多核苷酸的拷贝数差异的方法，特别是用于诊断胎儿遗传异常时，起始样品是母体血液，血清或血浆时的差异。

公开(公告)号：CA3041540A1

公开(公告)日：2011-03-24

申请号：CA3041540

申请日：2010-09-20

Applicant: BIO RAD LABORATORIES ,  AKONNI BIOSYSTEMS

Inventor： BELGRADER PHILLIP ,  HINDSON BENJAMIN

IPC: C12N1/06 ,  B01F7/16 ,  B01F13/08 ,  C12M1/33 ,  C12Q1/6806

Abstract: A Method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

公开(公告)号：ES2709123T3

公开(公告)日：2019-04-15

申请号：ES16166269

申请日：2010-09-20

Applicant: AKONNI BIOSYSTEMS ,  BIO RAD LABORATORIES

Inventor： BELGRADER PHILLIP ,  HINDSON BENJAMIN

IPC: B01F13/08 ,  B01F9/10 ,  B02C17/20 ,  C12M1/33 ,  C12N1/06 ,  C12N13/00

Abstract: Un método para lisar una o más células, que comprende: colocar una pluralidad de cámaras en una superficie, donde cada cámara comprende una o más células de interés, uno o más elementos de agitación magnética, y una pluralidad de perlas; y rotar un imán entre 1000 y 6000 rpm durante 90 a 120 segundos alrededor de un eje para producir un campo magnético de rotación, donde el eje es paralelo a la superficie sobre la cual residen las cámaras, la rotación del imán hace que uno o más agitadores magnéticos en las cámaras roten, lo que a su vez dirige el movimiento de las perlas, una o más células y el medio líquido y lisa las células dentro de las cámaras.

公开(公告)号：CA3041540C

公开(公告)日：2021-08-24

申请号：CA3041540

申请日：2010-09-20

Applicant: BIO RAD LABORATORIES ,  AKONNI BIOSYSTEMS

Inventor： BELGRADER PHILLIP ,  HINDSON BENJAMIN

IPC: C12N1/06 ,  B01F7/16 ,  B01F13/08 ,  C12M1/33 ,  C12Q1/6806

Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein, The container is dimensioned to allow rotation of the magnetic stir element inside the container.

公开(公告)号：EP2504448A4

公开(公告)日：2013-06-05

申请号：EP10833991

申请日：2010-11-25

Applicant: BIO RAD LABORATORIES

Inventor： HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY

IPC: C12P19/34

CPC classification number: C12Q1/6806 ,  C12Q1/6827 ,  C12Q2537/157 ,  C12Q2563/161 ,  C12Q2533/107 ,  C12Q2521/319

公开(公告)号：EP2675923A4

公开(公告)日：2015-07-01

申请号：EP12747706

申请日：2012-02-18

Applicant: BIO RAD LABORATORIES

Inventor： HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY ,  HODGES SHAWN PAUL ,  HEREDIA NICHOLAS J ,  MELLEN JEFFREY CLARK ,  TROUP CAMILLE BODLEY ,  WYATT PAUL

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2521/331 ,  C12Q2523/125 ,  C12Q2600/154

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma).