公开(公告)号：WO2012149042A3

公开(公告)日：2014-01-03

申请号：PCT/US2012035030

申请日：2012-04-25

Applicant: BIO RAD LABORATORIES ,  SAXONOV SERGE

Inventor： SAXONOV SERGE

IPC: C12Q1/68

CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1075 ,  C12Q1/6876 ,  C12Q2525/191 ,  C12Q2563/179

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

Abstract translation: 本文提供了用于测定的方法，组合物和试剂盒，其中许多涉及扩增反应，例如数字PCR或液滴数字PCR。 测定可以用于诸如测序，拷贝数变异分析等的应用。 在一些情况下，测定涉及将样品细分成多个分隔（例如，液滴），并将分区与包括具有条形码的适配器的其它分区合并。

公开(公告)号：AU2012231098A1

公开(公告)日：2013-10-03

申请号：AU2012231098

申请日：2012-03-19

Applicant: BIO RAD LABORATORIES

Inventor： SAXONOV SERGE ,  DUBE SIMANT ,  HINDSON BENJAMIN J ,  MCCOY ADAM M

IPC: C12Q1/68 ,  G01N21/00

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

公开(公告)号：WO2012112970A3

公开(公告)日：2013-11-14

申请号：PCT/US2012025760

申请日：2012-02-18

Applicant: BIO RAD LABORATORIES ,  HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY ,  HODGES SHAWN PAUL ,  HEREDIA NICHOLAS J ,  MELLEN JEFFREY CLARK ,  TROUP CAMILLE BODLEY ,  WYATT PAUL

Inventor： HINDSON BENJAMIN ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN ,  LUCERO MICHAEL ,  COLSTON BILLY ,  HODGES SHAWN PAUL ,  HEREDIA NICHOLAS J ,  MELLEN JEFFREY CLARK ,  TROUP CAMILLE BODLEY ,  WYATT PAUL

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6883 ,  C12Q2521/331 ,  C12Q2523/125 ,  C12Q2600/154

Abstract: Methods and compositions for detecting and quantifying polynucleotide in a sample. Specifically, methylated DNA is quantified to determine the load ofpolynucleotides in a sample. Non-invasive methods for determining the load of fetal polynucleotides in maternal plasma. Methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. Methods for detecting differences in copy number of a target polynucleotide, specifically, for diagnosis of fetal genetic abnormalities, when the starting sample is maternal blood, serum, or plasma.

Abstract translation: 用于检测和定量样品中多核苷酸的方法和组合物。 具体地，定量甲基化的DNA以确定样品中多核苷酸的负载。 用于确定母体血浆中胎儿多核苷酸负荷的非侵入性方法。 用于检测细胞过程如细胞活力，生长速率和感染率的方法和组合物。 用于检测目标多核苷酸的拷贝数差异的方法，特别是用于诊断胎儿遗传异常时，起始样品是母体血液，血清或血浆时的差异。

公开(公告)号：WO2012109500A3

公开(公告)日：2012-10-11

申请号：PCT/US2012024573

申请日：2012-02-09

Applicant: BIO RAD LABORATORIES ,  REGAN JOHN FREDERICK ,  SAXONOV SERGE ,  LUCERO MIKE ,  HINDSON BEN ,  BELGRADER PHIL ,  DUBE SIMANT ,  SO AUSTIN ,  MELLEN JEFFREY CLARK ,  HEREDIA NICHOLAS JACK ,  NESS KEVIN ,  COLSTON BILL

Inventor： REGAN JOHN FREDERICK ,  SAXONOV SERGE ,  LUCERO MIKE ,  HINDSON BEN ,  BELGRADER PHIL ,  DUBE SIMANT ,  SO AUSTIN ,  MELLEN JEFFREY CLARK ,  HEREDIA NICHOLAS JACK ,  NESS KEVIN ,  COLSTON BILL

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2537/165

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以实现样品中核酸的拷贝数估计。 本文还提供了用于确定样品中两个或更多个拷贝的靶核酸的连接（例如，两个或更多个拷贝是在相同染色体上还是不同染色体上）或用于定相等位基因的方法，组合物和试剂盒。

公开(公告)号：AU2012231098B2

公开(公告)日：2016-09-29

申请号：AU2012231098

申请日：2012-03-19

Applicant: BIO RAD LABORATORIES

Inventor： SAXONOV SERGE ,  DUBE SIMANT ,  HINDSON BENJAMIN J ,  MCCOY ADAM M

IPC: C12Q1/68 ,  G01N21/00

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

公开(公告)号：GB2485850A

公开(公告)日：2012-05-30

申请号：GB201100787

申请日：2010-11-25

Applicant: BIO RAD LABORATORIES

Inventor： HINDSON BENJAMIN JOSEPH ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN DEAN ,  LUCERO MICHAEL ,  COLSTON JR BILLY WAYNE

IPC: C12Q1/68

Abstract: Methods to determine nucleic acid copy numbers within a genetic material, such as chromosome number for the detection of foetal genetic or chromosomal diseases such as trisomy or tetraploidy in Downâ s syndrome, trisomy 8, Kleinfelterâ s syndrome, Patau syndrome, Edwards syndrome or XXX syndrome are disclosed wherein a first ligation probe (half-probe) specific for a first target sequence is hybridised to a target nucleic acid, a second ligation probe (half-probe) specific for a second target sequence is hybridised to an adjacent nucleic acid sequence and the two half-probes are joined by ligation prior to partitioning, preferably in an water-in-oil emulsion and subjected to quantitative amplification, preferably by a quantitative PCR approach. A microcapsule comprising a solid-phase exterior and an aqueous phase containing a ligated probe and a water-in-oil mixture comprising a ligation probes are also disclosed.

公开(公告)号：GB2485850B

公开(公告)日：2014-01-29

申请号：GB201100787

申请日：2010-11-25

Applicant: BIO RAD LABORATORIES

Inventor： HINDSON BENJAMIN JOSEPH ,  SAXONOV SERGE ,  BELGRADER PHILIP ,  NESS KEVIN DEAN ,  LUCERO MICHAEL ,  COLSTON BILLY WAYNE JR

IPC: C12Q1/68

公开(公告)号：CA2830443C

公开(公告)日：2021-11-16

申请号：CA2830443

申请日：2012-03-19

Applicant: BIO RAD LABORATORIES

Inventor： SAXONOV SERGE ,  DUBE SIMANT ,  HINDSON BENJAMIN J ,  MCCOY ADAM M

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6816 ,  C12Q1/6851 ,  C40B30/04 ,  G01N21/64

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay on a greater number of targets through combinatorial use of signals.

公开(公告)号：CA2826748C

公开(公告)日：2020-08-04

申请号：CA2826748

申请日：2012-02-09

Applicant: BIO-RAD LABORATORIES INC

Inventor： REGAN JOHN FREDERICK ,  SAXONOV SERGE ,  LUCERO MIKE ,  HINDSON BEN ,  BELGRADER PHIL ,  DUBE SIMANT ,  SO AUSTIN ,  MELLEN JEFFREY CLARK ,  HEREDIA NICHOLAS JACK ,  NESS KEVIN ,  COLSTON BILL

IPC: C12Q1/68 ,  C12Q1/6809 ,  C12Q1/683 ,  C12Q1/686 ,  C40B30/00

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g., whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

公开(公告)号：AU2012249759A1

公开(公告)日：2013-11-07

申请号：AU2012249759

申请日：2012-04-25

Applicant: BIO RAD LABORATORIES

Inventor： SAXONOV SERGE

IPC: C12Q1/68 ,  G01N21/64

Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.