公开(公告)号：EP2988872A4

公开(公告)日：2016-11-09

申请号：EP15780234

申请日：2015-04-17

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  KIM YOUNGJOON

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N33/54366 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2300/043 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2565/629 ,  C12Q2600/156

Abstract: Provided herein are cartridges, devices, and methods for carrying out multistep assays on a microfluidic scale. A cartridge includes a block frame comprising a well, wherein the well comprises an outlet, at least one inlet, and a bottom surface; a plurality of containers, wherein each container is connected to the well via a microchannel leading to the at least one inlet; and an openable cover, which cover when closed is configured to enclose an assay surface and form a gap between (i) the assay surface and the bottom surface of the well or (ii) the assay surface and the cover. The gap can be formed by a spacer that extends from the bottom surface of the well or from the outer edge of the cover. Methods include flowing liquid into the gap and/or through an opening adjacent to the assay surface.