公开(公告)号：CA2786432C

公开(公告)日：2016-03-22

申请号：CA2786432

申请日：2011-01-10

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN

IPC: G02B21/34 ,  G06M11/00

Abstract: Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber.

公开(公告)号：CA2786432A1

公开(公告)日：2011-07-21

申请号：CA2786432

申请日：2011-01-10

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN

IPC: G02B21/34

Abstract: Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber.

公开(公告)号：CA2832759A1

公开(公告)日：2012-11-15

申请号：CA2832759

申请日：2012-05-01

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  CHU DANIEL Y ,  PATT PAUL J ,  SADRI AMIR ,  TONG ROGER

IPC: B01L7/00

Abstract: Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

公开(公告)号：EP2836815A4

公开(公告)日：2015-11-11

申请号：EP13775579

申请日：2013-04-11

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  PATT PAUL J

IPC: G01N15/02 ,  B01L3/02 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract translation: 在受控条件下分配颗粒以通过使颗粒的悬浮液通过颗粒检测装置获得准确数量的颗粒，获得累积颗粒数，将累积颗粒数与目标值进行比较，并一旦关闭悬浮液流 粒子数达到目标值，全部以自动化方式进行。

公开(公告)号：EP2988872A4

公开(公告)日：2016-11-09

申请号：EP15780234

申请日：2015-04-17

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  KIM YOUNGJOON

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N33/54366 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2300/043 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2565/629 ,  C12Q2600/156

Abstract: Provided herein are cartridges, devices, and methods for carrying out multistep assays on a microfluidic scale. A cartridge includes a block frame comprising a well, wherein the well comprises an outlet, at least one inlet, and a bottom surface; a plurality of containers, wherein each container is connected to the well via a microchannel leading to the at least one inlet; and an openable cover, which cover when closed is configured to enclose an assay surface and form a gap between (i) the assay surface and the bottom surface of the well or (ii) the assay surface and the cover. The gap can be formed by a spacer that extends from the bottom surface of the well or from the outer edge of the cover. Methods include flowing liquid into the gap and/or through an opening adjacent to the assay surface.

公开(公告)号：EP3042178A4

公开(公告)日：2017-04-05

申请号：EP14841752

申请日：2014-09-05

Applicant: BIO-RAD LABORATORIES INC

Inventor： SADRI AMIR M ,  GUO KUN ,  CHU DANIEL Y ,  KIRCANSKI NENAD ,  PATT PAUL J ,  ROSENZWEIG TAL

IPC: G01N15/10 ,  B01L3/00

CPC classification number: G01N15/1459 ,  B01L3/0265 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1404 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract translation: 用于微流体操纵，分配和/或分选颗粒（例如细胞和/或珠）的系统，包括设备和方法。

公开(公告)号：EP2704835A4

公开(公告)日：2014-12-24

申请号：EP12782793

申请日：2012-05-01

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN ,  CHU DANIEL Y ,  PATT PAUL J ,  SADRI AMIR ,  TONG ROGER

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1855

公开(公告)号：EP2524262A4

公开(公告)日：2013-11-27

申请号：EP11733236

申请日：2011-01-10

Applicant: BIO RAD LABORATORIES

Inventor： GUO KUN

IPC: G02B21/34

CPC classification number: G02B21/34 ,  Y10S435/97 ,  Y10T436/112499