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    Chromatography cartridge
    4.
    发明专利
    Chromatography cartridge 未知

    公开(公告)号：GB2246716A

    公开(公告)日：1992-02-12

    申请号：GB9115674

    申请日：1991-07-19

    Applicant: BIO RAD LABORATORIES

    Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

    IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

    Abstract: A chromatographic method and apparatus for purifying proteins generally, and monoclonal antibodies from ascites fluids specifically, is disclosed. The method comprises contacting a solution containing proteins to be purified with a porous, substantially nonswellable sheet which has hydrated crystalline hydroxylapatite particles physically immobilized therein, in the presence of a pH buffer, and then eluting purified protein fractions, such that the flow rate and back pressure do not change significantly throughout the method. One preferred apparatus comprises inlet and outlet seals (10, 15), spiral- wound or core-wrapped chromatographic separation medium (60) comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet, and a Porous central support (40) upon which the sheet is wrapped. Another apparatus comprises single or stacked chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet.

    CHROMATOGRAPHY CARTRIDGE
    5.
    发明专利
    CHROMATOGRAPHY CARTRIDGE 未知

    公开(公告)号：CA2047902A1

    公开(公告)日：1992-02-08

    申请号：CA2047902

    申请日：1991-07-25

    Applicant: BIO RAD LABORATORIES

    Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

    IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60 ,  C07K1/18 ,  C07K16/00

    Abstract: 21 CHROMATOGRAPHY CARTRIDGE A chromatographic method and apparatus for purifying proteins generally, and monoclonal antibodies from ascites fluids specifically, achieves results superior to other methods. The method comprises contacting a solution containing proteins to be purified with a porous, substantially nonswellable sheet which has hydrated crystalline hydroxylapatite particles physically immobilized therein, in the presence of a pH buffer, and then eluting purified protein fractions, such that the flow rate and back pressure do not change significantly throughout the method. One preferred apparatus comprises inlet and outlet seals, spiral-wound or core-wrapped chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet, and a porous central support upon which the sheet is wrapped. Another apparatus comprises single or stacked chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet. PS\\DOCS\\5084

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