公开(公告)号：ITRM910595D0

公开(公告)日：1991-08-05

申请号：ITRM910595

申请日：1991-08-05

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

公开(公告)号：CA2807617C

公开(公告)日：2018-11-27

申请号：CA2807617

申请日：2011-08-17

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: C07K1/16 ,  C07K1/22 ,  C07K16/00

Abstract: Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.

公开(公告)号：CA2807617A1

公开(公告)日：2012-02-23

申请号：CA2807617

申请日：2011-08-17

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J

IPC: C07K1/16 ,  C07K1/22 ,  C07K16/00

Abstract: Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.

公开(公告)号：ITRM910595A1

公开(公告)日：1992-02-08

申请号：ITRM910595

申请日：1991-08-05

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D20060101 ,  B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

公开(公告)号：WO2006088607A3

公开(公告)日：2007-05-31

申请号：PCT/US2006002022

申请日：2006-01-17

Applicant: BIO RAD LABORATORIES ,  CUMMINGS LARRY J

Inventor： CUMMINGS LARRY J

IPC: B01D15/08

CPC classification number: B01D15/206 ,  B01D15/22 ,  G01N30/6021 ,  G01N30/6091 ,  G01N2030/565 ,  Y10T29/49 ,  Y10T29/496

Abstract: A chromatography cartridge with a packed bed is manufactured from an inner and outer chamber (11), the inner chamber being in an insert (12) that is slidable into the outer chamber (11) with openings in each such that a slurry that is originally placed in the interior of the outer chamber (11) as the parts are being pressed into each other. During the assembly, the particles in the slurry are compressed to fill the smaller volume of the inner chamber and excess liquid is discharged through ports in both chambers. The construction allows for a highly precise and reproducible column filling process with a minimum of steps and operator intervention and thereby low cost and reduced error.

Abstract translation: 具有填充床的色谱柱由内室和外室（11）制成，内腔位于可滑入外室（11）的插入件（12）中，每个开口均为原来的浆料 当部件被相互压入时，其被放置在外室（11）的内部。 在组装期间，浆料中的颗粒被压缩以填充较小体积的内室，并且多余的液体通过两个室中的端口排出。 该结构允许高度精确和可重复的柱填充过程，具有最小的步骤和操作者干预，从而降低成本并减少误差。

公开(公告)号：CA2787009A1

公开(公告)日：2011-07-21

申请号：CA2787009

申请日：2011-01-13

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  SNYDER MARK A

IPC: G01N30/02 ,  G01N33/544

Abstract: The present invention discloses methods of neutralizing apatite surfaces, for example during chromatography and before protein elution. Specifically, provided are methods for purifying a target molecule in a sample, comprising contacting a sample comprising the target molecule to an apatite solid surface, contacting the solid surface comprising the adsorbed target molecule with a solution comprising a basic amino compound and alkali metal ion or alkali earth ion; or a sulphonated amine compound and alkali metal ion or alkali earth ion, and eluting the target molecule from the solid support by contacting the solid support with a different solution.

公开(公告)号：FR2666244A1

公开(公告)日：1992-03-06

申请号：FR9110012

申请日：1991-08-06

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

公开(公告)号：GB2246716A

公开(公告)日：1992-02-12

申请号：GB9115674

申请日：1991-07-19

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60

Abstract: A chromatographic method and apparatus for purifying proteins generally, and monoclonal antibodies from ascites fluids specifically, is disclosed. The method comprises contacting a solution containing proteins to be purified with a porous, substantially nonswellable sheet which has hydrated crystalline hydroxylapatite particles physically immobilized therein, in the presence of a pH buffer, and then eluting purified protein fractions, such that the flow rate and back pressure do not change significantly throughout the method. One preferred apparatus comprises inlet and outlet seals (10, 15), spiral- wound or core-wrapped chromatographic separation medium (60) comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet, and a Porous central support (40) upon which the sheet is wrapped. Another apparatus comprises single or stacked chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet.

公开(公告)号：CA2047902A1

公开(公告)日：1992-02-08

申请号：CA2047902

申请日：1991-07-25

Applicant: BIO RAD LABORATORIES

Inventor： CUMMINGS LARRY J ,  TAYLOR MICHAEL A

IPC: B01D15/08 ,  C07K1/14 ,  C07K1/16 ,  C07K16/06 ,  G01N30/38 ,  G01N30/60 ,  C07K1/18 ,  C07K16/00

Abstract: 21 CHROMATOGRAPHY CARTRIDGE A chromatographic method and apparatus for purifying proteins generally, and monoclonal antibodies from ascites fluids specifically, achieves results superior to other methods. The method comprises contacting a solution containing proteins to be purified with a porous, substantially nonswellable sheet which has hydrated crystalline hydroxylapatite particles physically immobilized therein, in the presence of a pH buffer, and then eluting purified protein fractions, such that the flow rate and back pressure do not change significantly throughout the method. One preferred apparatus comprises inlet and outlet seals, spiral-wound or core-wrapped chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet, and a porous central support upon which the sheet is wrapped. Another apparatus comprises single or stacked chromatographic separation medium comprising hydrated crystalline hydroxylapatite particles physically immobilized in a porous, substantially nonswellable sheet. PS\\DOCS\\5084

公开(公告)号：WO2012024400A3

公开(公告)日：2012-05-10

申请号：PCT/US2011048082

申请日：2011-08-17

Applicant: BIO RAD LABORATORIES ,  CUMMINGS LARRY J

Inventor： CUMMINGS LARRY J

IPC: C07K1/00

CPC classification number: C07K1/18 ,  B01D15/361 ,  B01D15/3804 ,  B01D15/426 ,  C07K1/20 ,  C07K1/22

Abstract: Proteins, including monoclonal antibodies, that have been retained on hydroxyapatite resins for purposes of protein separation, purification, or both, are eluted from the resins by a elution buffer that contains controlled amounts of calcium and phosphate ions. The buffer allows elution to be performed in repeated runs at an acidic pH without deterioration of the resin.

Abstract translation: 通过含有受控量的钙和磷酸根离子的洗脱缓冲液，从树脂中洗脱已经保留在羟基磷灰石树脂上用于蛋白质分离，纯化或两者的蛋白质，包括单克隆抗体。 缓冲液允许在酸性pH下反复进行洗脱，而不会使树脂劣化。