公开(公告)号：JP2013152246A

公开(公告)日：2013-08-08

申请号：JP2013080058

申请日：2013-04-08

Applicant: Bio-Rad Laboratories Inc ,  バイオ−ラッド ラボラトリーズ インコーポレーティッド

Inventor： CRAIG ROWELL ,  CORY PANATTONI ,  THOMAS R BERKELMAN ,  SEAN CATER ,  SHANE PETERSEN ,  LEE OLECH ,  YANG XUEMEI

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: PROBLEM TO BE SOLVED: To provide a polyacrylamide gel capable of providing high resolution in protein separation and having higher stability to hydrolysis as compared with a conventional polyacrylamide gel depending on Tris or Tris-Bis as a buffer agent.SOLUTION: A polyacrylamide gel is produced by including triethanolamine instead of almost or all of Tris or Tris-Bis.

Abstract translation: 要解决的问题：与依赖于Tris或Tris-Bis作为缓冲剂的常规聚丙烯酰胺凝胶相比，提供能够提供高分辨率的蛋白质分离并且具有更高的水解稳定性的聚丙烯酰胺凝胶。溶液：产生聚丙烯酰胺凝胶 通过包括三乙醇胺代替几乎或全部的Tris或Tris-Bis。

公开(公告)号：CA2736107A1

公开(公告)日：2010-03-11

申请号：CA2736107

申请日：2009-09-02

Applicant: BIO RAD LABORATORIES

Inventor： ROWELL CRAIG ,  PANATTONI CORY ,  BERKELMAN THOMAS R ,  CATER SEAN ,  PETERSEN SHANE ,  OLECH LEE ,  YANG XUEMEI

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：CA2709213A1

公开(公告)日：2009-06-18

申请号：CA2709213

申请日：2008-12-08

Applicant: BIO RAD LABORATORIES

Inventor： YANG XUEMEI ,  PANATTONI CORY M

IPC: G01N33/559

Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester, polyester/cellulose blend, or polyurethane-coated cellulose wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.

公开(公告)号：EP2318839A4

公开(公告)日：2014-11-26

申请号：EP09812131

申请日：2009-09-02

Applicant: BIO RAD LABORATORIES

Inventor： ROWELL CRAIG ,  PANATTONI CORY ,  BERKELMAN THOMAS R ,  CATER SEAN ,  PETERSEN SHANE ,  OLECH LEE ,  YANG XUEMEI

IPC: G01N33/558 ,  B01D57/02 ,  G01N27/447 ,  G01N33/559 ,  G01N33/561

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

公开(公告)号：EP2220498A4

公开(公告)日：2011-01-05

申请号：EP08859533

申请日：2008-12-08

Applicant: BIO RAD LABORATORIES

Inventor： YANG XUEMEI ,  PANATTONI CORY M

IPC: G01N27/447 ,  C07K1/26 ,  G01N33/559

CPC classification number: G01N27/4473 ,  C07K1/26

公开(公告)号：EP2969141A4

公开(公告)日：2016-11-30

申请号：EP14769469

申请日：2014-03-10

Applicant: BIO RAD LABORATORIES

Inventor： LI LEI ,  YANG XUEMEI ,  BELISLE CHRISTOPHER

IPC: B01D57/02 ,  G01N27/447

CPC classification number: G01N27/44747 ,  B01D57/02 ,  B65D85/70 ,  C07K1/26 ,  C08F2/46 ,  C08K3/00 ,  G01N21/6428

Abstract: Hand cast gels, and solutions for their preparation, are described.