公开(公告)号：DE60114217T2

公开(公告)日：2006-07-13

申请号：DE60114217

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： ZHU MINGDE ,  OLECH LEE

IPC: G01N33/58 ,  G01N33/68 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：DE69926725D1

公开(公告)日：2005-09-22

申请号：DE69926725

申请日：1999-10-20

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  HARBERS J

IPC: G01N27/447 ,  B01D57/02

Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

公开(公告)号：AT548347T

公开(公告)日：2012-03-15

申请号：AT05705627

申请日：2005-01-12

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE

IPC: C07C279/12 ,  G01N27/447

公开(公告)号：CA2420149C

公开(公告)日：2006-10-31

申请号：CA2420149

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  ZHU MINGDE

IPC: G01N33/58 ,  G01N33/68 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves.

公开(公告)号：AU2001278141B2

公开(公告)日：2005-04-14

申请号：AU2001278141

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  ZHU MINGDE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68 ,  G01N033/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：CA2318542C

公开(公告)日：2003-08-05

申请号：CA2318542

申请日：1999-10-20

Applicant: BIO RAD LABORATORIES

Inventor： HARBERS ADRIANA J ,  OLECH LEE

IPC: G01N27/447 ,  B01D57/02

Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip (12) from atmospheric exposure and yet contains sufficient volumetric capacity (15) to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures (21, 22) each of whi ch is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures (23) through which lipid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap (24, 2 5, 26) that is removable to expose the aperture.

公开(公告)号：CA2736107A1

公开(公告)日：2010-03-11

申请号：CA2736107

申请日：2009-09-02

Applicant: BIO RAD LABORATORIES

Inventor： ROWELL CRAIG ,  PANATTONI CORY ,  BERKELMAN THOMAS R ,  CATER SEAN ,  PETERSEN SHANE ,  OLECH LEE ,  YANG XUEMEI

IPC: G01N33/558 ,  G01N33/559 ,  G01N33/561

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：DE05705627T1

公开(公告)日：2007-05-03

申请号：DE05705627

申请日：2005-01-12

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE

IPC: G01N27/26 ,  C07C279/00 ,  C07C279/12 ,  G01N27/447

公开(公告)号：DE60114217D1

公开(公告)日：2005-11-24

申请号：DE60114217

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： ZHU MINGDE ,  OLECH LEE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：AU1127900A

公开(公告)日：2000-06-13

申请号：AU1127900

申请日：1999-10-20

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  HARBERS ADRIANA J

IPC: G01N27/447 ,  B01D57/02

Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.