公开(公告)号：DE60114217D1

公开(公告)日：2005-11-24

申请号：DE60114217

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： ZHU MINGDE ,  OLECH LEE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：DE60114217T2

公开(公告)日：2006-07-13

申请号：DE60114217

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： ZHU MINGDE ,  OLECH LEE

IPC: G01N33/58 ,  G01N33/68 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：WO2007075325A2

公开(公告)日：2007-07-05

申请号：PCT/US2006047307

申请日：2006-12-11

Applicant: BIO RAD LABORATORIES ,  ZHU MINGDE ,  MOERSCHELL RICHARD P

Inventor： ZHU MINGDE ,  MOERSCHELL RICHARD P

IPC: B01D61/42 ,  G01N27/00

CPC classification number: G01N27/44704 ,  B01D57/02 ,  B01D69/12 ,  B01D2325/12 ,  B01D2325/20

Abstract: Proteins and other analytes that have been separated by electrophoretic means in a gel are transferred to a membrane by conventional blotting techniques, the membrane being a composite of an analyte binding layer and a size retention layer. The pore size of the size retention layer is large enough to allow non-macromolecular ions to pass, yet not large enough to allow the passage of the analytes from the gel, nor antibodies or other macromolecules or reagents in general that might be used in the detection, imaging, or quantification of the analytes.

Abstract translation: 通过电泳方法在凝胶中分离的蛋白质和其他分析物通过常规的印迹技术转移到膜中，该膜是分析物结合层和尺寸保持层的复合物。 尺寸保留层的孔径足够大以使非大分子离子通过，但不足以允许分析物从凝胶通过，也不允许抗体或其它大分子或试剂通常可用于 分析物的检测，成像或定量。

公开(公告)号：CA2420149C

公开(公告)日：2006-10-31

申请号：CA2420149

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  ZHU MINGDE

IPC: G01N33/58 ,  G01N33/68 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves.

公开(公告)号：AU2001278141B2

公开(公告)日：2005-04-14

申请号：AU2001278141

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  ZHU MINGDE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68 ,  G01N033/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：AU7814101A

公开(公告)日：2002-03-04

申请号：AU7814101

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： ZHU MINGDE ,  OLECH LEE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

公开(公告)号：CA2420149A1

公开(公告)日：2002-02-28

申请号：CA2420149

申请日：2001-08-03

Applicant: BIO RAD LABORATORIES

Inventor： OLECH LEE ,  ZHU MINGDE

IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves.