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公开(公告)号：JP2008259505A
公开(公告)日：2008-10-30
申请号：JP2008095922
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN , KROEGER BURKHARD , KIEFER PATRICK , HEINZLE ELMAR , WITTMANN CHRISTOPH
Abstract: PROBLEM TO BE SOLVED: To provide a method for accumulating and producing fine chemical materials (e.g., lysine) in a medium of an microorganism or the microorganism by increasing metabolic inflow through pentose phosphate route in the microorganism. SOLUTION: A method for increasing generation of fine chemical materials such as lysine from a microorganism, e.g., a corynebacterium by deregulating a ferment-encoding gene, i.e., fructose-1,6-bisphosphatase, is provided. Further, a new method for producing lysine by regulating carbon inflow directing to oxaloacetic acid (OAA), is also provided. In one a preferable embodiment, a method for producing lysine by using fructose or sucrose as the carbon source is provided. COPYRIGHT: (C)2009,JPO&INPIT
Abstract translation: 待解决的问题：提供一种通过在微生物中通过戊糖磷酸途径增加代谢流入而在微生物或微生物的培养基中积累和生产精细化学物质（例如赖氨酸）的方法。 解决方案：提供了通过使发酵编码基因（即果糖-1,6-二磷酸酶）失调来增加来自微生物例如棒状杆菌的精细化学物质如赖氨酸的产生的方法。 此外，还提供了通过调节引入草酰乙酸（OAA）的碳流入来生产赖氨酸的新方法。 在一个优选实施方案中，提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2008200046A
公开(公告)日：2008-09-04
申请号：JP2008095754
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： KLOPPROGGE CORINNA , ZELDER OSKAR , KROEGER BURKHARD , SCHROEDER HARTWIG , HAEFNER STEFAN , LIEBL WOLFGANG
CPC classification number: C12P13/04
Abstract: PROBLEM TO BE SOLVED: To provide methods for producing an amino acid, preferably of the group consisting of lysine, threonine, methionine, glutamate.
SOLUTION: The invention relates to the method for producing the amino acid comprising culturing a microorganism of the genus Corynebacterium or Brevibacterium wherein the microorganism is partially or completely deficient in at least one of the gene loci of the group which is formed by otsAB, treZ and treS, and subsequent isolation of the amino acid from the culture medium.
COPYRIGHT: (C)2008,JPO&INPITAbstract translation: 待解决的问题：提供优选由赖氨酸，苏氨酸，甲硫氨酸，谷氨酸组成的组的氨基酸的制备方法。 解决方案：本发明涉及生产氨基酸的方法，包括培养棒杆菌属或短杆菌属的微生物，其中微生物部分或完全缺乏由otsAB形成的基团的至少一个基因座 ，treZ和treS，然后从培养基中分离氨基酸。 版权所有（C）2008，JPO＆INPIT
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公开(公告)号：JP2008212156A
公开(公告)日：2008-09-18
申请号：JP2008095810
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： ZELDER OSKAR , KLOPPROGGE CORINNA , KROEGER BURKHARD , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: PROBLEM TO BE SOLVED: To provide the use of nucleic acid sequence for controlling the transcription and expression of genes, a new promoter and expression unit, a method for altering or arising the transcription speed and/or an expression speed of genes, the expression cassette including the expression unit, genetically altered microorganism having the altered or arisen transcription speed and/or expression speed, and a method for producing biosynthetic products by culturing the genetically altered microorganism. SOLUTION: There is provided a nucleic acid that has a specific sequence and a promoter activity, an expression unit including the nucleic acid, an expression cassette, an expression vector, a genetically altered microorganism, and a method for production of threonine by culturing the microorganism. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 要解决的问题：提供用于控制基因的转录和表达的核酸序列的使用，新的启动子和表达单位，改变或产生转录速度和/或基因的表达速度的方法， 具有表达单位的表达盒，具有改变或出现的转录速度和/或表达速度的遗传改变的微生物，以及通过培养遗传改变的微生物来生产生物合成产物的方法。 解决方案：提供了具有特定序列和启动子活性的核酸，包括核酸，表达盒，表达载体，遗传改变的微生物的表达单位和通过以下方法生产苏氨酸的方法： 培养微生物。 版权所有（C）2008，JPO＆INPIT
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公开(公告)号：JP2008212154A
公开(公告)日：2008-09-18
申请号：JP2008095699
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
IPC: C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/77 , C12P13/08 , C12R1/15
CPC classification number: C12N15/77
Abstract: PROBLEM TO BE SOLVED: To provide the use of a nucleic acid sequence for controlling the transcription and expression of a gene, to provide a new promoter and expression unit itself, to provide a method for modifying or inducing the transcription rate and/or the expression rate of the gene, to provide an expression cassette comprising the expression unit, to provide a genetically modified microorganism having the modified or induced transcription rate and/or expression rate and to provide a method for producing a biosynthetic product by culturing the genetically modified microorganism. SOLUTION: There is provided the use of (A) a specific nucleic acid sequence, (B) a sequence induced from the specific nucleic acid sequence by substitution, insertion or deletion of a nucleotide and having at least 90% homogeneity to the sequence at the nucleic acid level, (C) a nucleic acid sequence hybridizing to the nucleic acid sequence under stringent conditions or (D) a nucleic acid comprising a fragment functionally equivalent to the sequence (A), (B) or (C) and having a promoter activity. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 待解决的问题：提供用于控制基因的转录和表达的核酸序列的用途，以提供新的启动子和表达单元本身，以提供修饰或诱导转录速率和/ 或基因的表达速率，以提供包含表达单元的表达盒，以提供具有修饰或诱导的转录速率和/或表达率的遗传修饰微生物，并提供通过遗传培养生产生物合成产物的方法 修饰微生物。 提供（A）特定核酸序列的用途，（B）通过取代，插入或缺失核苷酸并且具有至少90％同一性的特异性核酸序列诱导的序列 （C）在严格条件下与核酸序列杂交的核酸序列或（D）包含与（A），（B）或（C）和（B）的序列功能等同的片段的核酸和 具有启动子活性。 版权所有（C）2008，JPO＆INPIT
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Patent Agency Ranking
公开(公告)号：JP2008237218A
公开(公告)日：2008-10-09
申请号：JP2008095696
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: PROBLEM TO BE SOLVED: To provide the use of nucleic acid sequences for regulating gene transcription and expression, a novel promoter and an expression unit itself, a method for modifying or inducing the gene transcription rate and/or expression rate, an expression cassette containing the expression unit, genetically modified microorganism having a modified or induced transcription rate and/or expression rate, and a method for producing biosynthetic products by culturing the genetically modified microorganism. SOLUTION: The use of a nucleic acid having promotor activity containing (A) a specific nucleic acid sequence for transcribing the gene, (B) a sequence having at least 90% homology and derived by substitution, insertion or deletion of nucleotides from the (A) sequence, (C) a nucleic acid sequence hybridizing under stringent conditions with the (A) sequence or (D) a functionally equivalent fragment of the sequence (A), (B) or (C) is provided. COPYRIGHT: (C)2009,JPO&INPIT
Abstract translation: 待解决的问题：提供用于调节基因转录和表达的核酸序列的使用，新型启动子和表达单元本身，修饰或诱导基因转录速率和/或表达率的方法，表达 含有表达单位的盒，具有修饰或诱导的转录速率和/或表达率的遗传修饰微生物，以及通过培养经遗传修饰的微生物生产生物合成产物的方法。 解决方案：使用具有启动子活性的核酸，其含有（A）用于转录该基因的特异性核酸序列，（B）具有至少90％同源性并通过其核苷酸取代，插入或缺失衍生的序列 （A）序列，（C）在严格条件下与（A）序列杂交的核酸序列或（D）序列（A），（B）或（C）的功能等同片段的核酸序列。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2008212155A
公开(公告)日：2008-09-18
申请号：JP2008095756
申请日：2008-04-02
Applicant: Basf Se , ビーエーエスエフ ソシエタス・ヨーロピアBasf Se
Inventor： KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
IPC: C12N15/09 , C07K14/34 , C12N20060101 , C12N1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/63 , C12N15/67 , C12N15/77 , C12P1/00 , C12P13/08 , C12P13/12 , C12R1/15
Abstract: PROBLEM TO BE SOLVED: To provide a nucleic acid sequence regulating the gene transcription and expression, to provide a new promoter and expression unit, to provide a method for altering or inducing the gene transcription rate and/or expression rate, to provide an expression cassette comprising the expression unit, to provide a genetically modified microorganism having the altered or the induced transcription rate and/or expression rate, and to provide a method for producing a biosynthetic product by culturing the genetically modified microorganism. SOLUTION: There are provided a nucleic acid sequence composed of a specific sequence and having a promoter activity, the expression unit comprising the nucleic acid, an expression vector comprising the expression unit and the method for preparing the biosynthetic product, particularly lysine by culturing the microorganism genetically modified with the nucleic acid having the promotor activity. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 待解决的问题：提供调节基因转录和表达的核酸序列，以提供新的启动子和表达单元，以提供改变或诱导基因转录速率和/或表达率的方法，以提供 包含表达单元的表达盒，提供具有改变的或诱导的转录速率和/或表达率的遗传修饰的微生物，并提供通过培养经遗传修饰的微生物来生产生物合成产物的方法。 提供了由特异性序列构成的具有启动子活性的核酸序列，包含核酸的表达单位，包含表达单位的表达载体和通过以下步骤制备生物合成产物的方法，特别是赖氨酸： 培养用具有启动子活性的核酸进行遗传修饰的微生物。 版权所有（C）2008，JPO＆INPIT
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公开(公告)号：AT531819T
公开(公告)日：2011-11-15
申请号：AT07123761
申请日：2005-07-16
Applicant: BASF SE
Inventor： ZELDER OSKAR , KLOPPROGGE CORINNA , KROEGER BURKHARD , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).
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公开(公告)号：ES2376035T3
公开(公告)日：2012-03-08
申请号：ES07123761
申请日：2005-07-16
Applicant: BASF SE
Inventor： ZELDER OSKAR , KLOPPROGGE CORINNA , KROEGER BURKHARD , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: Método para modificar o causar la rata de transcripción de genes en microorganismos en comparación con el tipo silvestre mediante regulación de la transcripción de genes en el microorganismo por ácidos nucleicos con actividad de promotor, que contiene A) la secuencia de ácido nucleico SEQ.ID.NO. 1 o B) una secuencia derivada de esta secuencia por sustitución, inserción o deleción de nucleótidos, que tiene una identidad de al menos 90 % a nivel de ácido nucleico con la secuencia SEQ.ID.NO. 1 o C) una secuencia de ácido nucleico que hibrida con la secuencia de ácido nucleico SEQ.ID.NO. 1 en condiciones estrictas, en cuyo caso los genes respecto de los ácidos nucleicos con actividad de promotor son heterólogos.
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公开(公告)号：ES2439946T3
公开(公告)日：2014-01-27
申请号：ES07123785
申请日：2004-12-15
Applicant: BASF SE
Inventor： KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
IPC: C12N15/77 , C07K14/34 , C12N20060101 , C12N1/00 , C12N1/21 , C12N15/63 , C12N15/67 , C12P1/00 , C12P13/08 , C12P13/12
Abstract: Proceso para aumentar el grado de transcripción de genes en microorganismos del género Corynebacterium encomparación con el tipo silvestre mediante regulación de la transcripción de genes en el microorganismo medianteácidos nucleicos con actividad promotora que contienen A) la secuencia de ácido nucleico SEQ.ID.NO. 1 o B) una secuencia derivada de esta secuencia mediante sustitución, inserción o deleción de nucleótidos, quepresenta una identidad de al menos 90 % a nivel de ácido nucleico con la secuencia SEQ.ID.NO. 1En cuyo caso los genes son heterólogos respecto de los ácidos nucleicos con actividad promotora.
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