公开(公告)号：WO2004040001A3

公开(公告)日：2004-07-22

申请号：PCT/US0331412

申请日：2003-10-02

Applicant: CALIFORNIA INST OF TECHN ,  HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH ,  QUAKE STEPHEN R ,  LEADBETTER JARED

Inventor： HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH ,  QUAKE STEPHEN R ,  LEADBETTER JARED

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

Abstract translation: 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。 随后纯化的核酸的洗脱之后可以连接和细胞转化，全部在相同的微流体芯片内。 在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

公开(公告)号：EP1546412A4

公开(公告)日：2007-10-03

申请号：EP03799829

申请日：2003-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH ,  QUAKE STEPHEN R ,  LEADBETTER JARED

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

Abstract translation: 来自各种环境的细胞和病毒的核酸可利用微流体技术进行纯化和表达。 根据本发明的一个实施方案，可以通过稀释，分选和/或分割在微流体室中分离单个或小群细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化所得的核酸。 随后纯化的核酸的洗脱可以在完全在相同的微流体芯片内进行连接和细胞转化。 在一个具体应用中，细胞分离，裂解和核酸纯化可以利用高度平行化的微流体结构来构建gDNA和cDNA文库。

公开(公告)号：AU2003299541A1

公开(公告)日：2004-05-25

申请号：AU2003299541

申请日：2003-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN R ,  LEADBETTER JARED ,  HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH

IPC: B01L3/00 ,  C12M1/34

Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. Individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. Cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

公开(公告)号：AU2003299541A8

公开(公告)日：2004-05-25

申请号：AU2003299541

申请日：2003-10-02

Applicant: CALIFORNIA INST OF TECHN

Inventor： QUAKE STEPHEN R ,  HONG JONG WOOK ,  LEADBETTER JARED ,  ANDERSON W FRENCH ,  STUDER VINCENT

IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H19/00

Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. Individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. Cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.