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    NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM
    2.
    发明申请
    NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM 审中-公开
    核酸和蛋白质及其制备和使用方法

    公开(公告)号：WO2008143679A8

    公开(公告)日：2009-01-29

    申请号：PCT/US2007070284

    申请日：2007-06-01

    Applicant: VERENIUM CORP ,  UNIV CALIFORNIA ,  CALIFORNIA INST OF TECHN ,  CHANG CATHY ,  MATHUR ERIC J ,  CAYOUETTE MICHELLE ,  ROBERTSON DAN E ,  HUGENHOLTZ PHILIP ,  WARNECKE FALK ,  LEADBETTER JARED ,  IVANOVA NATALIA ,  LUGINBUHL PETER ,  HUTCHISON DON

    Inventor： CHANG CATHY ,  MATHUR ERIC J ,  CAYOUETTE MICHELLE ,  ROBERTSON DAN E ,  HUGENHOLTZ PHILIP ,  WARNECKE FALK ,  LEADBETTER JARED ,  IVANOVA NATALIA ,  LUGINBUHL PETER ,  HUTCHISON DON

    IPC: C12N9/24

    CPC classification number: C12Y302/01015 ,  C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01039 ,  C12Y302/01067 ,  C12Y302/01082

    Abstract: The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

    Abstract translation: 本发明提供多肽，包括酶，结构蛋白和结合蛋白，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明的多肽，包括酶和抗体以及核酸可用于工业，实验，食品和饲料加工，营养和药物应用中，例如用于食品和饲料添加剂，着色剂，中性药物，化妆品和药物需求。 本发明的多肽可用于食品加工，酿造，浴添加剂，酒精生产，肽合成，对映选择性，皮革行业中的皮革制备，废物管理和动物降解，摄影工业中的银回收，医疗，丝绸脱胶， 生物膜降解，生物燃料转化为生物燃料（例如生物乙醇，生物甲醇，生物丙醇或生物丁醇，生物柴油等），生物防治剂，抗微生物剂和消毒剂，个人护理和化妆品，生物技术试剂，玉米湿法研磨和药物如 消化助剂和抗炎（抗炎）药剂。

    MICROFLUIDIC NUCLEIC ACID ANALYSIS
    4.
    发明公开
    MICROFLUIDIC NUCLEIC ACID ANALYSIS 有权
    MIKROFLUIDISCHENUKLEINSûUREANALYSE

    公开(公告)号：EP1546412A4

    公开(公告)日：2007-10-03

    申请号：EP03799829

    申请日：2003-10-02

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH ,  QUAKE STEPHEN R ,  LEADBETTER JARED

    IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    Abstract translation: 来自各种环境的细胞和病毒的核酸可利用微流体技术进行纯化和表达。 根据本发明的一个实施方案，可以通过稀释，分选和/或分割在微流体室中分离单个或小群细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化所得的核酸。 随后纯化的核酸的洗脱可以在完全在相同的微流体芯片内进行连接和细胞转化。 在一个具体应用中，细胞分离，裂解和核酸纯化可以利用高度平行化的微流体结构来构建gDNA和cDNA文库。

    Microfluidic nucleic acid analysis
    5.
    发明专利
    Microfluidic nucleic acid analysis 未知

    公开(公告)号：AU2003299541A8

    公开(公告)日：2004-05-25

    申请号：AU2003299541

    申请日：2003-10-02

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： QUAKE STEPHEN R ,  HONG JONG WOOK ,  LEADBETTER JARED ,  ANDERSON W FRENCH ,  STUDER VINCENT

    IPC: B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H19/00

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. Individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. Cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    MICROFLUIDIC NUCLEIC ACID ANALYSIS
    6.
    发明专利
    MICROFLUIDIC NUCLEIC ACID ANALYSIS 未知

    公开(公告)号：AU2003299541A1

    公开(公告)日：2004-05-25

    申请号：AU2003299541

    申请日：2003-10-02

    Applicant: CALIFORNIA INST OF TECHN

    Inventor： QUAKE STEPHEN R ,  LEADBETTER JARED ,  HONG JONG WOOK ,  STUDER VINCENT ,  ANDERSON W FRENCH

    IPC: B01L3/00 ,  C12M1/34

    Abstract: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. Individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. Cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

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