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    METHODS, DEVICES AND SYSTEMS FOR CHARACTERIZING PROTEINS
    2.
    发明申请
    METHODS, DEVICES AND SYSTEMS FOR CHARACTERIZING PROTEINS 审中-公开
    用于表征蛋白质的方法，装置和系统

    公开(公告)号：WO0046594A9

    公开(公告)日：2001-11-15

    申请号：PCT/US0002680

    申请日：2000-02-02

    Applicant: CALIPER TECHN CORP ,  DUBROW ROBERT S ,  BLOXSOM CHRISTOPHER ,  CHOW CALVIN Y H ,  PARCE JOHN WALLACE

    Inventor： DUBROW ROBERT S ,  BLOXSOM CHRISTOPHER ,  CHOW CALVIN Y H ,  PARCE JOHN WALLACE

    IPC: G01N27/447 ,  G01N37/00 ,  G01N27/26

    CPC classification number: G01N27/44747 ,  G01N27/44791

    Abstract: A method of characterizing a polypeptide, comprising providing a first capillary channel (104) having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solution, a buffering agent, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concentration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across the polymer solution at different rates. The polypeptide is then detected as it passes a point (176) along the length of the capillary channel.

    Abstract translation: 一种表征多肽的方法，包括提供其中设置有分离缓冲液的第一毛细通道（104），其中所述分离缓冲液包含非交联聚合物溶液，缓冲剂，洗涤剂和亲脂性染料。 提供分离缓冲液，使得在检测时，缓冲液中的洗涤剂浓度不高于临界胶束浓度。 将多肽引入毛细管通道的一端。 以不同的速率在整个聚合物溶液上施加电场。 然后当毛细通道沿着毛细通道的长度通过点（176）时，检测多肽。

    Methods, devices and systems for characterizing proteins
    7.
    发明专利
    Methods, devices and systems for characterizing proteins 未知

    公开(公告)号：AU758309B2

    公开(公告)日：2003-03-20

    申请号：AU3586900

    申请日：2000-02-02

    Applicant: CALIPER TECHN CORP

    Inventor： DUBROW ROBERT S ,  BLOXSOM CHRISTOPHER ,  CHOW CALVIN Y H ,  PARCE JOHN WALLACE

    IPC: G01N27/447 ,  G01N37/00 ,  G01N27/26

    Abstract: A method of characterizing a polypeptide, comprising providing a first capillary channel (104) having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solution, a buffering agent, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concentration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across the polymer solution at different rates. The polypeptide is then detected as it passes a point (176) along the length of the capillary channel.

    MICROPUMP
    8.
    发明专利
    MICROPUMP 未知

    公开(公告)号：CA2302675A1

    公开(公告)日：1999-04-01

    申请号：CA2302675

    申请日：1998-09-24

    Applicant: CALIPER TECHN CORP

    Inventor： PARCE JOHN WALLACE

    IPC: B01L3/00 ,  F04B19/00 ,  H02N11/00 ,  H02K44/00

    Abstract: The present invention generally provides a micropump (100) that utilizes electro-osmotic pumping of fluid in one channel or region (106) to generate a pressure based flow of material in a connected channel (104), where the connected channel (104) has substantially no electro-osmotic flow generated. Such pumps have a variety of applications, and are particularly useful in those situations where the application for which the pump is to be used prohibits the application of electric fields to the channel in which fluid flow is desired, or where pressure based flow is particularly desirable.

    HIGH-THROUGHPUT SCREENING ASSAY SYSTEMS IN MICROSCALE FLUIDIC DEVICES
    9.
    发明专利
    HIGH-THROUGHPUT SCREENING ASSAY SYSTEMS IN MICROSCALE FLUIDIC DEVICES 未知

    公开(公告)号：CA2258489C

    公开(公告)日：2004-01-27

    申请号：CA2258489

    申请日：1997-06-24

    Applicant: CALIPER TECHN CORP

    Inventor： PARCE JOHN WALLACE ,  BOUSSE LUC J ,  KOPF-SILL ANN R

    IPC: G01N31/20 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/25 ,  G01N27/26 ,  G01N27/27 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/574 ,  G01N35/08 ,  G01N37/00 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/02

    Abstract: The present invention provides microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. The device includes a series of channels (110, 112), and optional reagent channel (114), fabricated into the surface of the substrate. At least one of these channels will typically have very small cross-sectional dimensions, e.g. in the range of from about 0.1 hem to about 500 .mu.m. The device also includes reservoirs (104, 106 and 108), disposed and fluidly connected at the ends of the channels (110 and 114). As shown, sample channel (112) is used to introduce the plurality of different test compounds into the device. As such, this channel will generally be fluidly connected to a source of large numbers of separate test compounds that will be individually introduced into the sample channel (112) and subsequently into channel (110).

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