HYPERSPECTRAL VISIBLE ABSORPTION IMAGING OF MOLECULAR PROBES AND DYES IN BIOMATERIALS
    2.
    发明申请
    HYPERSPECTRAL VISIBLE ABSORPTION IMAGING OF MOLECULAR PROBES AND DYES IN BIOMATERIALS 审中-公开
    分子生物学中分子探针和染料的超视距可吸收成像

    公开(公告)号:WO2007038650A2

    公开(公告)日:2007-04-05

    申请号:PCT/US2006037780

    申请日:2006-09-26

    CPC classification number: G01N21/31

    Abstract: A system and method for obtaining hyperspectral visible absorption images. The system includes a light source which illuminates a sample containing light absorbing material, a platform, an optical lens, a detector. The platform has a section for placement of the sample and a section devoid of sample. The transmitted photons produced by the sample and collected by the optical lens are separated into a plurality of wavelength bands using a filter or a fiber array spectral translator coupled to a spectrometer. The system includes a programmable code for operating in a sample mode or background mode and calculating an absorption image. In the background mode, the platform motion is controlled so the optical lens collects transmitted photons from the portion of the platform devoid of sample to generate a background transmission image. In the sample mode, the platform motion is controlled so the optical lens collects transmitted photons from the sample to generate a sample transmission image. The sample transmission image and the background transmission image are used to generate a photon absorption image of the sample.

    Abstract translation: 一种用于获得高光谱可见吸收图像的系统和方法。 该系统包括照亮含有光吸收材料的样品的光源,平台,光学透镜,检测器。 该平台具有放置样品的部分和没有样品的部分。 由样品产生并由光学透镜收集的透射光子使用耦合到光谱仪的滤光器或光纤阵列光谱转换器分离成多个波长带。 该系统包括用于以采样模式或背景模式操作并计算吸收图像的可编程代码。 在背景模式中,控制平台运动,使得光学透镜从没有采样的平台的部分收集所发射的光子,以产生背景透射图像。 在采样模式中,控制平台运动,使得光学透镜从样本收集透射的光子以产生采样透射图像。 采样透射图像和背景透射图像用于产生样品的光子吸收图像。

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