中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

45 records (took 0.026 seconds)

    METHOD OF PREPARING DNA FRAGMENTS AND APPLICATIONS THEREOF
    1.
    发明申请
    METHOD OF PREPARING DNA FRAGMENTS AND APPLICATIONS THEREOF 审中-公开
    制备DNA片段的方法及其应用

    公开(公告)号：WO2004085678A3

    公开(公告)日：2004-12-09

    申请号：PCT/FR2004000671

    申请日：2004-03-18

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  BRACHET ANNE-GAELLE ,  RIZO PHILIPPE

    Inventor： BRACHET ANNE-GAELLE ,  RIZO PHILIPPE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q2525/131

    Abstract: The invention relates to a method of preparing DNA fragments and to the applications thereof, in particular for the hybridisation of nucleic acids. The inventive method is essentially characterised in that it consists of at least the following steps comprising: (a) preparation of double-stranded DNA fragments from a sample of nucleic acids to be analysed; (b) ligation of the ends of the aforementioned DNA fragments to a double-stranded oligonucleotide adapter (adapter AA') comprising the site for the recognition of a restriction enzyme of which the cleavage site is situated downstream of said recognition site; (c) amplification of the fragments linked to the above-mentioned adapter, using a pair of suitable primers, one of which is optionally marked at the 5' end thereof; and (d) cleavage of said DNA fragments close to one of the ends of same, using the restriction enzyme, such as to generate short fragments.

    Abstract translation: 本发明涉及制备DNA片段的方法及其应用，特别是用于核酸杂交的方法。 本发明的方法的基本特征在于其至少由以下步骤组成，所述步骤包括：（a）从待分析的核酸样品制备双链DNA片段; （b）将上述DNA片段的末端连接至包含识别限制性酶的位点的双链寡核苷酸接头（接头AA'），其中切割位点位于所述识别位点的下游; （c）使用一对合适的引物扩增与上述接头连接的片段，其中一个任选在其5'末端标记; 和（d）使用限制性内切酶切割靠近其一端的所述DNA片段，以产生短片段。

    METHOD OF MEASURING A QUANTITY OF PHOTONS WHICH IS PROPORTIONAL TO THE QUANTITY OF PHOTONS RECEIVED BY THE OBJECT AND ASSOCIATED DEVICE
    2.
    发明申请
    METHOD OF MEASURING A QUANTITY OF PHOTONS WHICH IS PROPORTIONAL TO THE QUANTITY OF PHOTONS RECEIVED BY THE OBJECT AND ASSOCIATED DEVICE 审中-公开
    测量由对象和相关设备接收的光子数量的光子数量的方法

    公开(公告)号：WO2004055502A3

    公开(公告)日：2004-08-12

    申请号：PCT/FR0350160

    申请日：2003-12-11

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  APIBIO ,  RIZO PHILIPPE

    Inventor： RIZO PHILIPPE

    IPC: G01N21/64

    CPC classification number: G01N21/6458 ,  G01N21/648

    Abstract: The invention relates to a fluorescence-measuring device comprising a photon-emitting source (1) which is intended to emit a photon beam in the direction of a sample to be analysed. The inventive device also comprises means (11) of detecting a fluorescence light which is emitted by the sample in response to the photons of the beam received by said sample. In addition, the device comprises: sampling means (3) which, during the fluorescence measuring process, can sample a determined fraction of the photon beam emitted by the photon source; means (4) of measuring the sampled fraction of the photon beam; and integration means (5) which can be used to integrate the determined fraction of the photon beam. The invention also relates to a method of obtaining a precise and repeatable estimation of the quantity of photons sent to the sample for a given measurement, using the inventive device. Said method comprises at least one comparison step whereby the value obtained by the measuring means (4) is compared with a reference value, said comparison producing a value known as the normalised fluorescence value.

    Abstract translation: 本发明涉及一种荧光测量装置，其包括光子发射源（1），其旨在沿待分析样品的方向发射光子束。 本发明的装置还包括检测由样品响应于由所述样品接收的光束的光子而发射的荧光的装置（11）。 此外，该装置包括：采样装置（3），其在荧光测量过程期间可以对由光子源发射的光子束的确定部分进行采样; 用于测量光子束采样分数的装置（4）; 和集成装置（5），其可用于整合确定的光子束分数。 本发明还涉及使用本发明的装置获得用于给定测量发送到样品的光子量的精确和可重复的估计的方法。 所述方法包括至少一个比较步骤，其中由测量装置（4）获得的值与参考值进行比较，所述比较产生称为标准化荧光值的值。

    METHOD OF PREPARING DNA FRAGMENTS BY SELECTIVE FRAGMENTATION OF NUCLEIC ACIDS AND APPLICATIONS THEREOF
    3.
    发明申请
    METHOD OF PREPARING DNA FRAGMENTS BY SELECTIVE FRAGMENTATION OF NUCLEIC ACIDS AND APPLICATIONS THEREOF 审中-公开
    通过核酸选择性分解制备DNA片段的方法及其应用

    公开(公告)号：WO2004094662A3

    公开(公告)日：2005-04-14

    申请号：PCT/FR2004000963

    申请日：2004-04-19

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  UNIV JOSEPH FOURIER ,  CENTRE NAT RECH SCIENT ,  BRACHET ANNE-GAELLE ,  RIZO PHILIPPE ,  TABERLET PIERRE ,  TEXIER-NOGUES ISABELLE

    Inventor： BRACHET ANNE-GAELLE ,  RIZO PHILIPPE ,  TABERLET PIERRE ,  TEXIER-NOGUES ISABELLE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q2525/131 ,  C12Q2521/313

    Abstract: The invention relates to a method of preparing DNA fragments by selective fragmentation of nucleic acid fragments. The inventive method comprises a first selection step involving selection of short fragments, consisting in: a) preparing first double-stranded DNA fragments F1 using at least one restriction enzyme E1 which can randomly fragment the nucleic acid sample to be analysed, by generating said DNA fragments F1 with blunt or cohesive ends; b) ligating the ends of the DNA fragments F1 obtained in step (a) to at least one adapter AA'; c) cleaving the DNA fragments F'1 obtained in step (b) using a restriction enzyme E2, such as to select a fraction of short fragments F2; and d) using any suitable means to purify the aforementioned fraction of short fragments F2. The inventive method also comprises the following optional step involving the second selection of one or more fragment sub-groups from the fraction of short fragments F2 obtained in step (d), said optional step consisting in: e) ligating the free end of the short fragments F2 obtained in step d) to at least one second complementary adapter BB' (production of fragments F'2); and f) amplifying short fragments F'2. The invention also relates to the applications of the above-mentioned method for the analysis of genomes and transcriptomes.

    Abstract translation: 本发明涉及通过选择性片段化核酸片段制备DNA片段的方法。 本发明的方法包括涉及选择短片段的第一选择步骤，其包括：a）使用至少一种限制酶E1制备第一双链DNA片段F1，所述限制酶E1可以通过产生所述DNA产生随机片段化的待分析的核酸样品 片段F1具有钝或粘性末端; b）将步骤（a）中获得的DNA片段F1的末端连接至至少一个衔接子AA'; c）使用限制酶E2切割步骤（b）中获得的DNA片段F'1，以选择一小部分短片段F2; 和d）使用任何合适的方法来纯化上述部分的短片段F2。 本发明的方法还包括以下任选步骤，其涉及从步骤（d）中获得的短片段F2的一个或多个片段亚组的第二选择，所述任选步骤包括：e）连接短片段的自由末端 步骤d）中获得的片段F2至少一个第二互补适配体BB'（片段F'2的产生）; 和f）放大短片段F'2。 本发明还涉及上述用于分析基因组和转录组的方法的应用。

    PROCEDE DE CORRECTION D’UNE IMAGE DE FLUORESCENCE
    4.
    发明专利
    PROCEDE DE CORRECTION D’UNE IMAGE DE FLUORESCENCE 未知

    公开(公告)号：FR3036187B1

    公开(公告)日：2019-09-13

    申请号：FR1554260

    申请日：2015-05-12

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  FLUOPTICS

    Inventor： RIZO PHILIPPE ,  DAURES ANTHONY

    IPC: G01N21/64 ,  A61B6/00 ,  G06F17/10

    Abstract: L'invention concerne un procédé pour corriger une image de fluorescence d'un objet, et en particulier d'un tissu biologique susceptible de comporter des agents fluorescents. Selon ce procédé, on mesure la distance entre une sonde de fluorescence, générant l'image de fluorescence et l'objet examiné. Cette distance est utilisée pour appliquer une fonction de correction à l'image de fluorescence. Un domaine d'application de l'invention est l'imagerie de fluorescence peropératoire pour le diagnostic et le suivi de l'évolution de pathologies, en particulier les cancers.

    PROCEDE DE CORRECTION D’UNE IMAGE DE FLUORESCENCE
    5.
    发明专利
    PROCEDE DE CORRECTION D’UNE IMAGE DE FLUORESCENCE 未知

    公开(公告)号：FR3036187A1

    公开(公告)日：2016-11-18

    申请号：FR1554260

    申请日：2015-05-12

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  FLUOPTICS

    Inventor： RIZO PHILIPPE ,  DAURES ANTHONY

    IPC: G01N21/64 ,  A61B6/00 ,  G06F17/10

    Abstract: L'invention concerne un procédé pour corriger une image de fluorescence d'un objet, et en particulier d'un tissu biologique susceptible de comporter des agents fluorescents. Selon ce procédé, on mesure la distance entre une sonde de fluorescence, générant l'image de fluorescence et l'objet examiné. Cette distance est utilisée pour appliquer une fonction de correction à l'image de fluorescence. Un domaine d'application de l'invention est l'imagerie de fluorescence peropératoire pour le diagnostic et le suivi de l'évolution de pathologies, en particulier les cancers.

    6.
    发明专利
    未知

    公开(公告)号：DE602004017980D1

    公开(公告)日：2009-01-08

    申请号：DE602004017980

    申请日：2004-04-19

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  UNIV JOSEPH FOURIER ,  CENTRE NAT RECH SCIENT

    Inventor： BRACHET ANNE-GAELLE ,  RIZO PHILIPPE ,  TABERLET PIERRE ,  TEXIER-NOGUES ISABELLE

    IPC: C12Q1/68

    Abstract: Method for preparing fragments (F) of DNA from a sample of nucleic acids being analyzed, by selective fragmentation. Method for preparing fragments (F) of DNA from a sample of nucleic acids being analyzed, by selective fragmentation comprises at least one of the steps: (1) a first selection of short fragments by producing first double-stranded DNA fragments (F1), using at least one restriction enzyme (E1) that cuts in a random manner to generate free or cohesive ends; (2) ligating the ends of F1 to an adapter (AA') to form a motif (M) (at the junction of the complementary strand of AA' and the 5'-end of F1) where the sequence of (M) is N-x first base pairs (bp) from the recognition site (of N bp) of a restriction enzyme (E2) for which the cutting site is downstream of the recognition site (x is between 1 and N+1), and the 3'-end (situated in the 5'-region of F1) is the restriction site for E1, resulting in fragments F'1; (3) cutting F'1 close to their 5'-ends, using E2, to select a fraction of short fragments F2 and these are purified by any standard method; (4) a second selection of one or more sub-sets of F2 by ligating the free end (not bound to AA') to a second complementary adapter (BB') to form fragments F'2; (5) amplifying F'2 linked to both adapters, using appropriate primers, at least one being optionally 5'-labeled, so as to select at least one subset of F'2. Independent claims are also included for the following: (1) DNA fragment (A1) that represents a genetic marker and prepared by the new method; (2) DNA chip that carries (A1); (3) method for hybridization of nucleic acid, using (A1) or the chip of (2); (4) kit for method (3) containing (A1) or the chip of (2); and (5) kit for performing the new method that contains at least one adapter AA'.

    PROCEDE ET DISPOSITIF DE LOCALISATION DE FLUOROPHORES OU D'ABSORBEURS DANS UN MILIEU ENVIRONNANT
    10.
    发明专利
    PROCEDE ET DISPOSITIF DE LOCALISATION DE FLUOROPHORES OU D'ABSORBEURS DANS UN MILIEU ENVIRONNANT 未知

    公开(公告)号：FR2938181A1

    公开(公告)日：2010-05-14

    申请号：FR0857699

    申请日：2008-11-13

    Applicant: COMMISSARIAT ENERGIE ATOMIQUE

    Inventor： DA SILVA ANABELA ,  DINTEN JEAN MARC ,  RIZO PHILIPPE

    IPC: A61B5/00

    Abstract: L'invention concerne un procédé de localisation d'un fluorophore (22) dans un milieu diffusant (20), à l'aide d'une source (8, 10) de rayonnement apte à émettre un rayonnement d'excitation de ce fluorophore et des moyens (4, 12) de détection apte à mesurer un signal de fluorescence (Φ ) émis par ce fluorophore (22) comportant: a) pour au moins 3 couples différents de positions de la source de rayonnement et des moyens de détection, une excitation par un rayonnement provenant de la source (8) de rayonnement, et une détection par les moyens (4) de détection du signal de fluorescence émis par ce fluorophore après cette excitation, b) pour chacun de ces couples, l'identification d'une surface sur laquelle le fluorophore est situé, ou d'un volume comprenant cette surface et dans lequel le fluorophore est situé, c) une estimation de la localisation du fluorophore dans son milieu environnant, par calcul de l'intersection des trois surfaces, ou éventuellement d'un volume autour de cette intersection.

Patent Agency Ranking