公开(公告)号：JP2000131236A

公开(公告)日：2000-05-12

申请号：JP30152499

申请日：1999-10-22

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： PELTIE PHILIPPE ,  DAVID DOMINIQUE

IPC: G01N21/64 ,  G02B21/06

Abstract: PROBLEM TO BE SOLVED: To provide a decoder suitable for a chip having a small number of small-size contacts with sufficient intervals between the contact by determining the quantity of fluorescence of a contact to decide a density of substance mixed with the fluorescent material. SOLUTION: An image acquisition means 4 is focused on a matrix disposed and fixed below an optical axis of a microscope 1, and a reference contact aligned to the optical center of this system is automatically retrieved, while an image of one contact of the matrix is acquired. The system has a calculation part for calculating fluorescence of the contact and deducing the density of substance. An illuminating means has a ring-shaped light source 11 and a laser light source 13 for exciting a fluorescent material contained in a mixture constituting the substance. The system also has a means for recording the image of the contact and a processing means 7 for analyzing the image. Thereby, the system can determine a quantity of the fluorescence of the contact to decide the density of the substance mixed with the fluorescent material.

公开(公告)号：JP2002072142A

公开(公告)日：2002-03-12

申请号：JP2001236615

申请日：2001-08-03

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： PELTIE PHILIPPE ,  CAMPAGNOLO RAYMOND ,  BERGER MICHEL

IPC: G01N21/64 ,  G02B27/28

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent imaging device capable of easily discriminating molecules which are different in size. SOLUTION: This fluorescent imaging device comprises the first means in which a component to be analyzed is put, the second means which illuminates the component to be analyzed by polarization and the third means which measures fluorescence emitting from the component under the effect of the polarization. In this case, characteristically, the first means comprises a parallel micro channel structure (4), and the second means has at least one combining device (2, 5) which guides polarized light to the micro channel.

公开(公告)号：JPH11127900A

公开(公告)日：1999-05-18

申请号：JP19640298

申请日：1998-07-10

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CAILLAT PATRICE ,  PELTIE PHILIPPE ,  LIVACHE THIERRY

IPC: C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N21/66 ,  G01N27/02 ,  G01N27/327 ,  G01N33/487 ,  G01N33/53 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide an analyzer capable of practicing analysis having reliability and reproducibility by a chip comprising many electrodes coated with different reagents or probe molecules and capable of individually heating to prescribed temperatures. SOLUTION: This analyzer comprises at least one chip (110) equipped with plural analytic electrodes (112). The device is further equipped with a means (150) for individually heating the analytic electrodes. The analyzer can be applied to analysis of chemical or biological products and can be applied to e.g. antigen/antibody reaction or DNA/DNA analysis.

公开(公告)号：WO02065160A3

公开(公告)日：2002-12-12

申请号：PCT/FR0200435

申请日：2002-02-05

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  PELTIE PHILIPPE ,  DEROU-MADELINE DOMINIQUE ,  CAMPAGNOLO RAYMOND

Inventor： PELTIE PHILIPPE ,  DEROU-MADELINE DOMINIQUE ,  CAMPAGNOLO RAYMOND

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/04 ,  G02B21/16

CPC classification number: G02B21/04 ,  G02B21/16

Abstract: The invention concerns a device for microscopic observation of at least a sample (2) such as a biological sample, arranged on a support (6), said device comprising at least a light source (42) for illuminating the sample, means (48) for reflecting said light towards the sample, a catadioptric lens (38) for observing the sample, said lens forming an image of the sample, and means (52) for acquiring said image. The reflecting means are arranged between the lens and the support.

Abstract translation: 本发明涉及用于显微观察至少一个布置在支撑体（6）上的生物样品（2）的样品（2）的装置，所述装置至少包括用于照射样品的光源（42），装置（48） 用于将所述光反射到样品，用于观察样品的反射折射透镜（38），所述透镜形成样品的图像，以及用于获取所述图像的装置（52）。 反射装置设置在透镜和支架之间。

公开(公告)号：WO2006087437A3

公开(公告)日：2007-04-26

申请号：PCT/FR2006000131

申请日：2006-01-20

Applicant: COMMISSARIAT ENERGIE ATOMIQUE ,  PELTIE PHILIPPE ,  BERGER MICHEL ,  SAUZE ROLAND ,  BONNET STEPHANE

Inventor： PELTIE PHILIPPE ,  BERGER MICHEL ,  SAUZE ROLAND ,  BONNET STEPHANE

IPC: G01N21/64

CPC classification number: G01N21/64 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/0638

Abstract: The inventive device comprises a first light source and a first wavelength (?1) corresponding to an excitation wavelength (?Ex) of a fluorophore. The excitation wavelength (?Ex) and an emission wavelength (?Em) of the fluorophore delimit a predetermined interval (?Em). The device also comprises a second light source having a second wavelength (?2) offset with regard to the first wavelength (?1) in such a manner that it is outside said predetermined interval (?Em). The offset (?12) between the first (?1) and second (?2) wavelengths is between 30 nm and 100 nm. A camera is provided comprising a filter opaque to the first (?1) and second (?2) wavelengths and transparent to the emission wavelength (?Em) and to the wavelengths noticeably greater than the highest of the first (?1) and second (?2) wavelengths. The light sources and the camera are synchronized for alternately activating one of the light sources and enabling the camera to alternately acquire a fluorescence image and a background noise image.

Abstract translation: 本发明的装置包括对应于荧光团的激发波长（λEx）的第一光源和第一波长（λ1）。 荧光团的激发波长（ΔEx）和发射波长（ΔEm）限定预定间隔（ΔEm）。 该装置还包括具有相对于第一波长（θ1）偏移的第二波长（λ2）的第二光源，使得其在所述预定间隔（ΔEm）之外。 第一（λ1）和第二（λ2）波长之间的偏移（θ12）在30nm和100nm之间。 提供一种照相机，包括对第一（λ1）和第二（λ2）波长不透明并且对于发射波长（θEm）透明的滤光器和明显大于第一（λ1）和第二 （λ2）波长。 光源和照相机被同步以交替地激活光源之一并且使相机能够交替地获取荧光图像和背景噪声图像。

公开(公告)号：DE602007000275D1

公开(公告)日：2009-01-08

申请号：DE602007000275

申请日：2007-04-03

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： TEXIER-NOGUES ISABELLE ,  PELTIE PHILIPPE ,  HEINRICH EMILIE ,  BLANC ROLANDE

IPC: A61B6/00 ,  A61B5/02

Abstract: The method involves introducing a fluorescent marker e.g. antibody/fluorophore conjugate, into a biological tissue e.g. organ. The marker is excited by incident light radiations, where the marker is sequentially excited at different incident excitation wavelengths. Bands of emission relating to fluorescence emitted by the marker are detected in response to the excitation. Intensities of fluorescence relative to the emission bands are analyzed. The marker is based on a group of fluorophores that are excited by the wavelengths and based on an up-converting semiconductor inorganic nanocrystal.

公开(公告)号：FR2916339A1

公开(公告)日：2008-11-28

申请号：FR0703738

申请日：2007-05-25

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： BOUTET JEROME ,  PELTIE PHILIPPE

IPC: A61B19/00

Abstract: Le domaine général de l'invention est celui des sondes optiques per-opératoires destinées à aider le chirurgien dans son geste médical.Les sondes optiques à fluorescence selon l'invention sont destinées à être utilisées sur des tissus vivants où les zones malades ont été marquées par un marqueur fluorescent. Elles possèdent un double éclairage. Le premier (1, 2) situé dans le rouge ou le proche infra-rouge est nécessaire pour réaliser la fluorescence des zones marquées (30) et obtenir une image exploitable par une caméra (7). Le second (9, 10) situé dans le visible est nécessaire pour éclairer en lumière visible les zones marquées, facilitant ainsi le travail du chirurgien. L'éclairage visible peur être soit ponctuel, soit être réalisé par un projecteur d'images. Dans ce dernier cas, l'image projetée éclaire uniquement les zones malades.

公开(公告)号：FR2900043B1

公开(公告)日：2008-07-04

申请号：FR0603610

申请日：2006-04-24

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： TEXIER NOGUES ISABELLE ,  PELTIE PHILIPPE ,  HEINRICH EMILIE ,  BLANC ROLANDE

IPC: A61B5/00

Abstract: The method involves introducing a fluorescent marker e.g. antibody/fluorophore conjugate, into a biological tissue e.g. organ. The marker is excited by incident light radiations, where the marker is sequentially excited at different incident excitation wavelengths. Bands of emission relating to fluorescence emitted by the marker are detected in response to the excitation. Intensities of fluorescence relative to the emission bands are analyzed. The marker is based on a group of fluorophores that are excited by the wavelengths and based on an up-converting semiconductor inorganic nanocrystal.

公开(公告)号：FR2900043A1

公开(公告)日：2007-10-26

申请号：FR0603610

申请日：2006-04-24

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： TEXIER NOGUES ISABELLE ,  PELTIE PHILIPPE ,  HEINRICH EMILIE ,  BLANC ROLANDE

IPC: A61B5/00

Abstract: La présente invention concerne un procédé d'imagerie optique par fluorescence d'au moins un tissu biologique, notamment pour délimiter des régions d'intérêt du ou des tissu(s) à analyser par tomographie.Le procédé selon l'invention comprend les étapes suivantes :a) l'introduction d'au moins un marqueur fluorescent dans le(s) tissu(s),b) l'excitation du ou de chaque marqueur par des rayonnements lumineux incidents et une détection de bandes d'émission relatives à des rayonnements fluorescents émis par le ou chaque marqueur en réponse à cette excitation, puisc) l'analyse de la fluorescence de ces bandes d'émission,et il est caractérisé en ce que l'étape b) comprend :- une excitation séquentielle, par n longueurs d'onde d'excitation lambdai incidentes différentes, du ou de chaque marqueur apte à être excité par au moins deux de ces lambdai et à émettre en réponse, pour chaque lambdai, une série Si de m bandes d'émission Bj simultanées de différentes longueurs d'onde lambda'j qui sont sensiblement les mêmes d'une série Si à une autre (où n et m >= 2, et où i et j varient de 1 à n et de 1 à m), et- une détection de ces séries, pour en déduire une estimation de la localisation tridimensionnelle du ou de chaque marqueur dans le(s) tissu(s) et/ou des coefficients d'absorption moyens de tissu(s) vis-à-vis des excitations lambdai.

公开(公告)号：FR2805348B1

公开(公告)日：2002-07-12

申请号：FR0002236

申请日：2000-02-23

Applicant: COMMISSARIAT ENERGIE ATOMIQUE

Inventor： CUZIN MARC ,  PELTIE PHILIPPE ,  FONTECAVE MARC ,  DECOUT JEAN LUC ,  DUEYMES CECILE

IPC: G01N21/64 ,  C12Q1/68 ,  C12Q1/6837 ,  C40B40/06 ,  G01N21/78 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/533 ,  G01N33/68 ,  B01L3/00

Abstract: Analytical support (A), which is fixed to a carrier, comprises many oligonucleotides (ON), each of which is labeled by a fluorescent compound (I), the fluorescence of which varies when ON hybridizes to its complement, is new. Independent claims are also included for the following: (1) the quality control of (A) in which the amounts and positions of the fixed ONs are determined by measuring the fluorescence from (I); an (2) analyzing biological targets by applying them to (A) and identifying those ON which have hybridized by measuring fluorescence from each ON before and after contact with the targets.