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    METHOD FOR PRODUCING FINE CHEMICALS
    2.
    发明申请
    METHOD FOR PRODUCING FINE CHEMICALS 审中-公开
    生产精细化学品的方法

    公开(公告)号：WO03023016A2

    公开(公告)日：2003-03-20

    申请号：PCT/EP0210174

    申请日：2002-09-11

    Applicant: DEGUSSA ,  FARWICK MIKE ,  HERMANN THOMAS

    Inventor： FARWICK MIKE ,  HERMANN THOMAS

    IPC: C12N1/15 ,  C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/88 ,  C12P13/08 ,  C12N1/00

    CPC classification number: C12N9/93 ,  C12N9/0006 ,  C12N9/88 ,  C12P13/08

    Abstract: The present invention relates to genetically modified microorganisms, in particular coryneform bacteria and Escherichia coli, in which the phosphorylation state of proteins or polypeptides has been changed such that the bacteria produce a larger amount of a desired fine chemical or a metabolite than the wild type, and to a method for the production of fine chemicals or metabolites.

    Abstract translation: 本发明涉及基因修饰的微生物，特别是棒状杆菌和大肠杆菌，其中蛋白质或多肽的磷酸化状态已经改变，使得细菌产生比野生型更大量的期望的精细化学品或代谢物， 并涉及生产精细化学品或代谢物的方法。

    FERMENTATION PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING STRAINS OF THE FAMILY ENTEROBACTERIACEAE
    3.
    发明申请
    FERMENTATION PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING STRAINS OF THE FAMILY ENTEROBACTERIACEAE 审中-公开
    使用家禽肠杆菌菌株制备L-氨基酸的发酵方法

    公开(公告)号：WO0229080A3

    公开(公告)日：2002-09-26

    申请号：PCT/EP0110209

    申请日：2001-09-05

    Applicant: DEGUSSA

    Inventor： RIEPING MECHTHILD ,  BASTUCK CHRISTINE ,  HERMANN THOMAS ,  THIERBACH GEORG

    IPC: C12N15/52 ,  C12N15/60 ,  C12P13/06 ,  C12P13/08 ,  C12P13/04 ,  C12N1/21 ,  C12N15/11

    CPC classification number: C12R1/19 ,  C12N15/52 ,  C12P13/06 ,  C12P13/08

    Abstract: The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out: a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms at least the pckA gene and/or the open reading frames yjfA and ytfP are, individually or jointly, attenuated and, in particular, switched off, b) enrichment of the L-amino acid in the medium or in the bacterial cells, and c) isolation of the L-amino acid.

    Abstract translation: 本发明涉及一种用于制备L-氨基酸，特别是L-苏氨酸的发酵方法，其中进行以下步骤：a）产生所需L-氨基酸的肠杆菌科的微生物发酵，其中 至少pckA基因和/或开放阅读框yjfA和ytfP的微生物单独或共同减弱，特别是关闭，b）富集培养基或细菌细胞中的L-氨基酸，以及 c）分离L-氨基酸。

    PROCESS FOR THE PREPARATION OF L-AMINO ACIDS BY USING CORYNEFORM BACTERIA
    9.
    发明申请
    PROCESS FOR THE PREPARATION OF L-AMINO ACIDS BY USING CORYNEFORM BACTERIA 审中-公开
    通过使用CORYNEFORM BACTERIA制备L-氨基酸的方法

    公开(公告)号：WO02074966A2

    公开(公告)日：2002-09-26

    申请号：PCT/EP0202568

    申请日：2002-03-08

    Applicant: DEGUSSA

    Inventor： FARWICK MIKE ,  BATHE BRIGITTE ,  HERMANN THOMAS ,  PFEFFERLE WALTER

    IPC: C12N1/21 ,  C12N9/88 ,  C12N15/60 ,  C12P13/08 ,  C12N1/20 ,  C12P13/04

    CPC classification number: C12R1/15 ,  C12N9/88 ,  C12P13/08

    Abstract: The invention relates to a process for the preparation of L-amino acids, wherein the following steps are implemented: a) fermentation of the coryneform bacteria producing the desired L-amino acid, in which at least the fda gene is attenuated, b) enrichment of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the L-amino acid, and optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.

    Abstract translation: 本发明涉及一种制备L-氨基酸的方法，其中实施以下步骤：a）产生所需L-氨基酸的棒状细菌的发酵，其中至少fda基因被减弱，b）富集 在培养基或细菌细胞中的所需L-氨基酸，和c）分离L-氨基酸，并且任选地使用细菌，其中另外还需要所需L的生物合成途径的其它基因 - 氨基酸被增强，或者使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地被切断。

    SEQUENCES WHICH CODE FOR THE SIGE GENE
    10.
    发明申请
    SEQUENCES WHICH CODE FOR THE SIGE GENE 审中-公开
    序列符号代码

    公开(公告)号：WO0218428A2

    公开(公告)日：2002-03-07

    申请号：PCT/EP0108146

    申请日：2001-07-14

    Applicant: DEGUSSA

    Inventor： MOECKEL BETTINA ,  HERMANN THOMAS ,  FARWICK MIKE ,  BINDER MICHAEL ,  PFEFFERLE WALTER

    IPC: C07K14/34 ,  C12N1/21 ,  C12P13/08 ,  C07K14/195

    CPC classification number: C12R1/15 ,  C07K14/34 ,  C12P13/08

    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    Abstract translation: 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码多肽的多核苷酸的程度相同，所述多核苷酸包含SEQ ID No的氨基酸序列 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sigE 基因以增强形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

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