公开(公告)号：JP2001025368A

公开(公告)日：2001-01-30

申请号：JP2000185071

申请日：2000-06-20

Applicant: DEGUSSA

Inventor： BINDER MICHAEL ,  UFFMANN KLAUS-ERICH

IPC: A23K1/16 ,  A23K1/00 ,  A23K1/175

Abstract: PROBLEM TO BE SOLVED: To obtain a supplement for feed, having deactivated microorganisms from fermentation, capable of promoting metabolism and based on a fermentation-treated liquid by including L-lysin (salt), a biomass formed during the fermentation, or the like. SOLUTION: This supplement for feed contains (A) L-lysin (salt), preferably of 10-35 wt.% (based on the total amount of a solution or a suspension), (g) a biomass formed during the fermentation of 0-100 wt.%, preferably 50-100 wt.%, especially preferably 90-100 wt.%, and (C) at least a large amount of the content of other dissolved and suspended contents of the fermentation treated liquid. The supplement is present in a shape of (D) the liquid or optionally the suspension, and has (E) a pH value lower than 7, preferably of 2-6.5. The solution or suspension containing the lysin (salt) is obtained by producing the L-lysin-containing treated liquid by fermentation, regulating the pH of the obtained treated liquid so as to be

公开(公告)号：JP2000325024A

公开(公告)日：2000-11-28

申请号：JP2000132558

申请日：2000-05-01

Applicant: DEGUSSA

Inventor： BINDER MICHAEL ,  UFFMANN KLAUS-ERICH ,  WALGER ILONA ,  BECKER ULRICH ,  PFEFFERLE WALTER ,  FRIEDRICH HEINZ

IPC: A23K1/00 ,  A23K1/16 ,  A23K1/175 ,  C12P13/02

Abstract: PROBLEM TO BE SOLVED: To profitably and efficiently obtain an animal feed additive having excellent flowability by adding an alkali (ne earth)metal hydroxide, or the like, to a fermentation broth containing D-pantothenic acid(salt) and then subjecting the mixture to a spray-drying treatment, or the like. SOLUTION: This animal feed additive is obtained by perfectly or partially separating a biomass and/or several other components from a fermentation broth containing D-pantothenic acid and/or one or more salts thereof selected from the group consisting of sodium, potassium, ammonium, magnesium and calcium salt thereof, adding an alkali (ne earth) metal hydroxide or oxide to the residual broth, and then (spray-)drying or (spray-)granulating the mixture to obtain the solid, particulate or granular easily flowable animal feed additive. The (hydr)oxide of the metal selected from the group consisting of the sodium, potassium, ammonium, magnesium and calcium compounds is preferably added in a stoichiometric ratio of 0.8 to 1.2 to the D-pantothenic acid.

公开(公告)号：WO0218428A2

公开(公告)日：2002-03-07

申请号：PCT/EP0108146

申请日：2001-07-14

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  HERMANN THOMAS ,  FARWICK MIKE ,  BINDER MICHAEL ,  PFEFFERLE WALTER

IPC: C07K14/34 ,  C12N1/21 ,  C12P13/08 ,  C07K14/195

CPC classification number: C12R1/15 ,  C07K14/34 ,  C12P13/08

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract translation: 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码多肽的多核苷酸的程度相同，所述多核苷酸包含SEQ ID No的氨基酸序列 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sigE 基因以增强形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

公开(公告)号：WO0210209A8

公开(公告)日：2002-04-04

申请号：PCT/EP0108220

申请日：2001-07-17

Applicant: DEGUSSA

Inventor： BATHE BRIGITTE ,  MOECKEL BETTINA ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  RUECKERT CHRISTIAN ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

IPC: A23K1/16 ,  C12N1/21 ,  C12N9/10 ,  C12P13/12 ,  C07K14/34 ,  A23L1/305 ,  C12N15/10 ,  C12N15/63 ,  C12P13/08 ,  C12Q1/68

CPC classification number: C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15 ,  C12R1/19

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene coding for 5 -methyltetrahydrofolate homocysteine methyltransferase (EC 2.1.1.13) is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

Abstract translation: 本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含SEQ ID NO： 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与 ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因 编码5-甲基四氢叶酸高半胱氨酸甲基转移酶（EC 2.1.1.13）以增强形式存在，并且使用多核苷酸序列作为杂交探针。

公开(公告)号：WO0218430A3

公开(公告)日：2002-07-04

申请号：PCT/EP0108221

申请日：2001-07-17

Applicant: DEGUSSA

Inventor： BATHE BRIGITTE ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  RUECKERT CHRISTIAN ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

IPC: C07K14/34 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

CPC classification number: C12P13/12 ,  C07K14/34 ,  C12N9/88 ,  C12P13/08

Abstract: The invention relates to polynucleotides from coryneform bacteria which code for the metR and/or metZ genes and comprise polynucleotide sequences, chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 3, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 3, e) polynucleotide which is complementary to the polynucleotides of a) b), c) or d), and f) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b), c), d) or e) and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metR gene and/or the metZ gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract translation: 本发明涉及编码metR和/或metZ基因的来自棒状细菌的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多核苷酸的多核苷酸至少70％相同的多核苷酸， 包含SEQ ID No.2的氨基酸序列的多肽，b）与编码包含SEQ ID No.3的氨基酸序列的多肽的多核苷酸的至少70％同一性的多核苷酸，c ）多核苷酸，其编码包含与SEQ ID No.2的氨基酸序列的至少70％的同一性的氨基酸序列的多肽，d）编码包含氨基酸序列的多肽的多核苷酸 其与SEQ ID No.3的氨基酸序列的至少70％的同一性程度相同，e）与a）b），c）或d）和f）多核苷酸的多核苷酸互补的多核苷酸 其包含a），b），c），d）或e）的多核苷酸序列的至少15个连续核苷酸以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少该metR基因和/或 metZ基因以减毒形式存在，并且使用包含根据本发明的序列作为杂交探针的多核苷酸。

公开(公告)号：WO2005093080A3

公开(公告)日：2006-04-27

申请号：PCT/EP2005002134

申请日：2005-03-01

Applicant: DEGUSSA

Inventor： OSSWALD STEFFEN ,  VERSECK STEFAN ,  DEITING UTA ,  WECKBECKER CHRISTOPH ,  HUTHMACHER KLAUS ,  BINDER MICHAEL ,  KULA MARIA-REGINA ,  ODENDAHL KONRAD

IPC: C12P13/02 ,  C07K14/47 ,  C12N9/78 ,  C12N9/88

CPC classification number: C07K14/47 ,  C12N9/88 ,  C12P13/02

Abstract: The invention relates to a polynucleotide cluster of Rhodococcus, containing nucleotide sequences coding for polypeptides having the activity of a nitrile hydratase, an auxiliary protein P15K activating said enzyme, and a cobalt transporter. The invention also relates to micro-organisms transformed by said cluster, in which the nucleotide sequences coding for said proteins are reinforced, and to the use of the transformed micro-organisms for producing amides from nitriles.

Abstract translation: 本发明涉及从红球菌一个Polynucleotidcluster，具有这种酶激活辅助蛋白P15K和转运编码钴核苷酸序列的腈水合酶的活性，所述多肽包含转化的，其中用于这些蛋白质的编码核苷酸序列的至少扩增的微生物和利用所述转化的微生物用于生产 从腈酰胺。

公开(公告)号：WO02066651A2

公开(公告)日：2002-08-29

申请号：PCT/EP0200573

申请日：2002-01-22

Applicant: DEGUSSA

Inventor： MOECKEL BETTINA ,  BATHE BRIGITTE ,  HANS STEPHAN ,  KREUTZER CAROLINE ,  HERMANN THOMAS ,  PFEFFERLE WALTER ,  BINDER MICHAEL

IPC: C07K14/34 ,  C12N15/31

CPC classification number: C07K14/34

Abstract: The invention relates to an isolated polynucleotide coding for the ribosomal protein S12 from Corynebacterium glutamicum and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the rpsL gene is present in enhanced form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract translation: 本发明涉及编码来自谷氨酸棒杆菌的核糖体蛋白S12的分离的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少rpsL基因以增强形式存在，以及使用多核苷酸 包括根据本发明的序列作为杂交探针。

公开(公告)号：WO0218430A8

公开(公告)日：2004-03-04

申请号：PCT/EP0108221

申请日：2001-07-17

Applicant: DEGUSSA

Inventor： BATHE BRIGITTE ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  RUECKERT CHRISTIAN ,  KALINOWSKI JOERN ,  PUEHLER ALFRED ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

IPC: C07K14/34 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

CPC classification number: C12P13/12 ,  C07K14/34 ,  C12N9/88 ,  C12P13/08

Abstract: The invention relates to polynucleotides from coryneform bacteria which code for the metR and/or metZ genes and comprise polynucleotide sequences, chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 3, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 3, e) polynucleotide which is complementary to the polynucleotides of a) b), c) or d), and f) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b), c), d) or e) and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metR gene and/or the metZ gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract translation: 本发明涉及来自棒状细菌的多核苷酸，其编码metR和/或metZ基因，并且包含选自以下的多核苷酸序列：a）与编码 多肽，其包含SEQ ID No.2的氨基酸序列，b）与编码包含SEQ ID No.3的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸，c ）多核苷酸，其编码包含与SEQ ID No.2的氨基酸序列至少70％相同程度的氨基酸序列的多肽，d）编码包含氨基酸序列的多肽的多核苷酸 其与SEQ ID No.3的氨基酸序列至少70％的程度相同，e）与a）b），c）或d）的多核苷酸互补的多核苷酸，和f）多核苷酸 包括a），b），c），d）或e）的多核苷酸序列的至少15个连续核苷酸的方法，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metR基因和/或 metZ基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

公开(公告)号：WO0229029A3

公开(公告)日：2002-06-13

申请号：PCT/EP0109723

申请日：2001-08-23

Applicant: DEGUSSA

Inventor： FARWICK MIKE ,  HUTHMACHER KLAUS ,  SCHISCHKA NATALIE ,  BATHE BRIGITTE ,  PFEFFERLE WALTER ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

IPC: A23K1/00 ,  A23K1/16 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12 ,  C12P13/04 ,  C12Q1/68

CPC classification number: C12N9/0051 ,  A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C12N9/1029 ,  C12N9/1241 ,  C12N9/88 ,  C12P13/08 ,  C12P13/12

Abstract: The invention provides nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.

Abstract translation: 本发明提供了编码cysD，cysN，cysK，cysE和cysH基因的棒状细菌的核苷酸序列，以及使用其中提及的基因增强的细菌发酵制备氨基酸的方法，L的发酵制备方法 使用棒状细菌，其中至少cysD基因，cysN基因，cysK基因，cysE基因和/或cysH基因以增强形式存在，并且使用含有根据本发明的序列的多核苷酸 作为杂交探针和从发酵液中制备含有L-甲硫氨酸的动物饲料添加剂的方法。

公开(公告)号：WO0210206A3

公开(公告)日：2002-05-02

申请号：PCT/EP0108224

申请日：2001-07-17

Applicant: DEGUSSA

Inventor： BATHE BRIGITTE ,  MOECKEL BETTINA ,  PFEFFERLE WALTER ,  HUTHMACHER KLAUS ,  BINDER MICHAEL ,  GREISSINGER DIETER ,  THIERBACH GEORG

IPC: A23K1/00 ,  A23K1/16 ,  C07K14/34 ,  C12N1/21 ,  C12N9/06 ,  C12P13/12 ,  A23L1/305 ,  C12N15/10 ,  C12N15/63 ,  C12P13/08 ,  C12Q1/68

CPC classification number: C12N9/0044 ,  A23K10/12 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metF gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

Abstract translation: 本发明涉及分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.的氨基酸序列的多肽的多核苷酸至少70％相同的程度的多核苷酸 2的多核苷酸，b）编码包含与SEQ ID No.2的氨基酸序列的至少70％的同一性的氨基酸序列的多肽的多核苷酸，c）与SEQ ID No.2的多核苷酸互补的多核苷酸， ）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，和使用棒状细菌发酵制备L-氨基酸的方法，其中至少metF基因 以增强形式存在，以及使用多核苷酸序列作为杂交探针。