公开(公告)号：DE50005837D1

公开(公告)日：2004-05-06

申请号：DE50005837

申请日：2000-02-08

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： MARX DR ,  MOECKEL DR ,  PFEFFERLE DR ,  SAHM DR ,  DE GRAAF DR ,  EGGELING DR

IPC: C12N9/06 ,  C12N15/53 ,  C12P13/06 ,  C12P13/08 ,  C12P13/22 ,  C12P13/24

Abstract: This invention relates to a process for the production of L-amino acids using coryneform bacteria, in which the glutamate dehydrogenase gene is amplified.

公开(公告)号：DE50010097D1

公开(公告)日：2005-05-25

申请号：DE50010097

申请日：2000-11-04

Applicant: DEGUSSA

Inventor： MOECKEL DR ,  PFEFFERLE DR ,  MARX DR ,  KALINOWSKI DR ,  BATHE DR ,  PUEHLER PROF

IPC: C12N15/09 ,  C07H21/00 ,  C07K14/34 ,  C12N1/21 ,  C12N9/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/53 ,  C12N15/77 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/15

Abstract: Isolated polynucleotide (I) is the new zwa1 gene from Corynebacterium glutamicum. Isolated zwa1 polynucleotide (I) contains a sequence that is: (i) at least 70% identical with a sequence that encodes a 199 amino acid (aa) sequence (II), given in the specification; (ii) encodes a polypeptide at least 70% identical with (II); (iii) is the complement of (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) from (i)-(iii). Independent claims are also included for the following: (1) vectors containing (I); (2) coryneform bacterial host cells containing the vector of (1) or having the zwa1 gene amplified; and (3) production of L-amino acids, especially L-lysine, by growing an aa-producing bacterium in which at least the zwa1 gene has been amplified.