公开(公告)号：DE60015042T3

公开(公告)日：2008-03-06

申请号：DE60015042

申请日：2000-07-25

Applicant: EVONIK DEGUSSA GMBH

Inventor： KIRCHNER OLIVER ,  BATHE BRIGITTE DR ,  MOECKEL BETTINA DR ,  HARTMANN MICHAEL ,  KALINOWSKI JOERN DR ,  PUEHLER PROF ,  PFEFFERLE WALTER DR

IPC: C12N15/09 ,  C12N15/61 ,  C12N1/21 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for the polypeptide which is expressed by the dapF gene contained on vector pEC-XT99A-dapF in the deposited C.glutamicum strain DSM 12969, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which is complementary to the polynucleotides of a), b) or c), and e) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d), and a process for the fermentative preparation of L-Lysin with amplification of the dapF gene.

公开(公告)号：DE50010097D1

公开(公告)日：2005-05-25

申请号：DE50010097

申请日：2000-11-04

Applicant: DEGUSSA

Inventor： MOECKEL DR ,  PFEFFERLE DR ,  MARX DR ,  KALINOWSKI DR ,  BATHE DR ,  PUEHLER PROF

IPC: C12N15/09 ,  C07H21/00 ,  C07K14/34 ,  C12N1/21 ,  C12N9/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/53 ,  C12N15/77 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/15

Abstract: Isolated polynucleotide (I) is the new zwa1 gene from Corynebacterium glutamicum. Isolated zwa1 polynucleotide (I) contains a sequence that is: (i) at least 70% identical with a sequence that encodes a 199 amino acid (aa) sequence (II), given in the specification; (ii) encodes a polypeptide at least 70% identical with (II); (iii) is the complement of (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) from (i)-(iii). Independent claims are also included for the following: (1) vectors containing (I); (2) coryneform bacterial host cells containing the vector of (1) or having the zwa1 gene amplified; and (3) production of L-amino acids, especially L-lysine, by growing an aa-producing bacterium in which at least the zwa1 gene has been amplified.

公开(公告)号：DE60015042D1

公开(公告)日：2004-11-25

申请号：DE60015042

申请日：2000-07-25

Applicant: DEGUSSA

Inventor： KIRCHNER OLIVER ,  BATHE DR ,  MOECKEL DR ,  HARTMANN MICHAEL ,  KALINOWSKI DR ,  PUEHLER PROF ,  PFEFFERLE DR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/61 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for the polypeptide which is expressed by the dapF gene contained on vector pEC-XT99A-dapF in the deposited C.glutamicum strain DSM 12969, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which is complementary to the polynucleotides of a), b) or c), and e) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d), and a process for the fermentative preparation of L-Lysin with amplification of the dapF gene.