公开(公告)号：DE50005837D1

公开(公告)日：2004-05-06

申请号：DE50005837

申请日：2000-02-08

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： MARX DR ,  MOECKEL DR ,  PFEFFERLE DR ,  SAHM DR ,  DE GRAAF DR ,  EGGELING DR

IPC: C12N9/06 ,  C12N15/53 ,  C12P13/06 ,  C12P13/08 ,  C12P13/22 ,  C12P13/24

Abstract: This invention relates to a process for the production of L-amino acids using coryneform bacteria, in which the glutamate dehydrogenase gene is amplified.

公开(公告)号：DE50010097D1

公开(公告)日：2005-05-25

申请号：DE50010097

申请日：2000-11-04

Applicant: DEGUSSA

Inventor： MOECKEL DR ,  PFEFFERLE DR ,  MARX DR ,  KALINOWSKI DR ,  BATHE DR ,  PUEHLER PROF

IPC: C12N15/09 ,  C07H21/00 ,  C07K14/34 ,  C12N1/21 ,  C12N9/00 ,  C12N15/11 ,  C12N15/31 ,  C12N15/53 ,  C12N15/77 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/15

Abstract: Isolated polynucleotide (I) is the new zwa1 gene from Corynebacterium glutamicum. Isolated zwa1 polynucleotide (I) contains a sequence that is: (i) at least 70% identical with a sequence that encodes a 199 amino acid (aa) sequence (II), given in the specification; (ii) encodes a polypeptide at least 70% identical with (II); (iii) is the complement of (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) from (i)-(iii). Independent claims are also included for the following: (1) vectors containing (I); (2) coryneform bacterial host cells containing the vector of (1) or having the zwa1 gene amplified; and (3) production of L-amino acids, especially L-lysine, by growing an aa-producing bacterium in which at least the zwa1 gene has been amplified.

公开(公告)号：DE50000123D1

公开(公告)日：2002-04-25

申请号：DE50000123

申请日：2000-05-10

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： TILG YVONNE ,  EIKMANNS PROF DR ,  EGGELING DR ,  SAHM PROF DR ,  MOECKEL DR ,  PFEFFERLE DR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/18 ,  C12N9/88 ,  C12N15/52 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12R1/15

Abstract: Production of L-amino acids comprises culturing a coryneform bacterium that overexpress the accBC gene.

公开(公告)号：DE60015042D1

公开(公告)日：2004-11-25

申请号：DE60015042

申请日：2000-07-25

Applicant: DEGUSSA

Inventor： KIRCHNER OLIVER ,  BATHE DR ,  MOECKEL DR ,  HARTMANN MICHAEL ,  KALINOWSKI DR ,  PUEHLER PROF ,  PFEFFERLE DR

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/61 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for the polypeptide which is expressed by the dapF gene contained on vector pEC-XT99A-dapF in the deposited C.glutamicum strain DSM 12969, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which is complementary to the polynucleotides of a), b) or c), and e) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d), and a process for the fermentative preparation of L-Lysin with amplification of the dapF gene.

公开(公告)号：DE50000778D1

公开(公告)日：2003-01-02

申请号：DE50000778

申请日：2000-04-26

Applicant: DEGUSSA

Inventor： BINDER DR ,  UFFMANN KLAUS-ERICH ,  WALGER DR ,  BECKER DR ,  PFEFFERLE DR ,  FRIEDRICH DR

IPC: A23K1/16 ,  C12P13/02

公开(公告)号：DE50011356D1

公开(公告)日：2006-03-02

申请号：DE50011356

申请日：2000-07-06

Applicant: DEGUSSA ,  FORSCHUNGSZENTRUM JUELICH GMBH

Inventor： KREUTZER CAROLINE ,  MOECKEL DR ,  PFEFFERLE DR ,  EGGELING DR ,  SAHM PROF ,  PATEK MIROSLAV

IPC: C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70 ,  C12N15/77 ,  C12P13/08 ,  C12R1/15

Abstract: New L-lysine producing coryneform bacteria (A) contain amplified pyc (pyruvate carboxylase) and dapA (dihydrodipicolinate) genes, where the promoter of the dapA gene contains the mutation MC20 or MA16 (as shown in respective sequences of 79 or 80 bases given in the specification as SEQ-ID-NO: 5 and SEQ-ID-NO: 6). (A) optionally also contains amplified lycC, lysE and/or dapB gene(s). An independent claim is included for the preparation of L-lysine by fermentation of coryneform bacteria having an amplified pyc gene, where the bacteria additionally contain the amplified dapA gene as specified for (A).

公开(公告)号：DE59609469D1

公开(公告)日：2002-08-29

申请号：DE59609469

申请日：1996-04-20

Applicant: DEGUSSA

Inventor： SCHILLING BERNHARD ,  PFEFFERLE DR ,  BACHMANN DR ,  LEUCHTENBERGER PROF DR ,  DECKWER PROF DR W-D

IPC: C12M1/04 ,  C12M1/06

Abstract: This reactor has disc stirrers and a vol. of 10-500 l. It is novel in being fitted with at least one horizontal perforated disc above and/or below the other discs. Also claimed is use of such a reactor to investigate mass conversion and biomass prodn. during fermentation and/or enzymatic processes. It may be used to determine appropriate mixing times in reactors of vol. ≤ 100 m . Also claimed is a procedure for the modification of stirred reactors with disc stirrers, substantially in accordance with the methods described.