公开(公告)号：WO2010083250A3

公开(公告)日：2010-11-18

申请号：PCT/US2010020942

申请日：2010-01-13

Applicant: FLUIDIGM CORP ,  HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

Abstract translation: 本发明提供了从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后进行预扩增和扩增选定的靶核酸。 分析RNA的方法可能需要所需RNA的逆转录，然后进行预扩增和扩增选择的靶核酸。

公开(公告)号：EP2379753A4

公开(公告)日：2012-05-30

申请号：EP10732056

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：SG183029A1

公开(公告)日：2012-08-30

申请号：SG2012052031

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: SINGLE-CELL NUCLEIC ACID ANALYSISThe present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.Fig. 1

公开(公告)号：SG172965A1

公开(公告)日：2011-08-29

申请号：SG2011050739

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：EA020593B1

公开(公告)日：2014-12-30

申请号：EA201170933

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

Abstract: Изобретениеотноситсяк способаманализагеномнойДНКи/илиРНКизнебольшихобразцовилидажеединичныхклеток. СпособыанализагеномнойДНКмогутвключатьполногеномнуюамплификацию (WGA) споследующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней. СпособыанализаРНКмогутвключатьобратнуютранскрипциюжелательнойРНКс последующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней.