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公开(公告)号：WO2011143659A3
公开(公告)日：2012-07-12
申请号：PCT/US2011036670
申请日：2011-05-16
Applicant: FLUIDIGM CORP , MAY ANDREW , MIR ALAIN , RAMAKRISHNAN RAMESH , ZIMMERMANN BERNHARD
Inventor： MAY ANDREW , MIR ALAIN , RAMAKRISHNAN RAMESH , ZIMMERMANN BERNHARD
CPC classification number: C12P19/34 , C07H21/00 , C12Q1/6806 , C12Q1/6869 , C12Q2600/118 , C12Q2565/113 , C12Q2527/125 , C12Q2525/204 , C12Q2525/307 , C12Q2525/15 , C12Q2521/507
Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.
Abstract translation: 本发明提供了用于选择性富集来自癌症患者的生物样品中的母体样品中的短核酸例如胎儿DNA的生物样品或随机分析用于基因型，突变和/ 或非整倍体。
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公开(公告)号：WO2011142836A9
公开(公告)日：2012-01-05
申请号：PCT/US2011000887
申请日：2011-05-16
Applicant: FLUIDIGM CORP , JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
Inventor： JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
Abstract translation: 本发明提供了用于检测基因型，突变和/或非整倍性的基于扩增的方法。 这些方法具有广泛的适用性，但特别适合于检测和定量存在于母体液样品中的游离胎儿DNA中的靶核酸。
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公开(公告)号：WO2010088288A2
公开(公告)日：2010-08-05
申请号：PCT/US2010022258
申请日：2010-01-27
Applicant: FLUIDIGM CORP , DUBE SIMANT , MIR ALAIN , RAMAKRISHNAN RAMESH , WEAVER LESLEY SUZANNE , ZIMMERMANN BERNHARD G
Inventor： DUBE SIMANT , MIR ALAIN , RAMAKRISHNAN RAMESH , WEAVER LESLEY SUZANNE , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6853 , C12Q1/6809 , C12Q1/6886 , C12Q2600/16 , C12Q2561/113 , C12Q2545/10 , C12Q2527/107
Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。
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公开(公告)号：WO2011142836A3
公开(公告)日：2012-07-05
申请号：PCT/US2011000887
申请日：2011-05-16
Applicant: FLUIDIGM CORP , JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
Inventor： JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , PIEPRZYK MARTIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.
Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。
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公开(公告)号：WO2010083250A3
公开(公告)日：2010-11-18
申请号：PCT/US2010020942
申请日：2010-01-13
Applicant: FLUIDIGM CORP , HAMILTON AMY , LIN MIN , MIR ALAIN , PIEPRZYK MARTIN
Inventor： HAMILTON AMY , LIN MIN , MIR ALAIN , PIEPRZYK MARTIN
CPC classification number: C12Q1/6881 , C12Q1/6844 , C12Q1/686 , C12Q2531/113 , C12Q2531/119 , C12Q2531/137 , C12Q2565/101 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C12Q2600/178
Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
Abstract translation: 本发明提供了从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后进行预扩增和扩增选定的靶核酸。 分析RNA的方法可能需要所需RNA的逆转录，然后进行预扩增和扩增选择的靶核酸。
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Patent Agency Ranking
公开(公告)号：WO2010027870A3
公开(公告)日：2010-07-01
申请号：PCT/US2009055083
申请日：2009-08-26
Applicant: FLUIDIGM CORP , MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/686 , C12N15/1065 , C12Q1/6853 , Y10T436/143333 , C12Q2537/143 , C12Q2525/161
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。
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公开(公告)号：CA2734868C
公开(公告)日：2019-09-10
申请号：CA2734868
申请日：2009-08-26
Applicant: FLUIDIGM CORP
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
IPC: C12Q1/68 , C12Q1/6844 , C12Q1/6848 , C40B70/00
Abstract: The present disclosure relates to methods for detecting a plurality of target nucleic acids in a plurality of samples comprising providing S samples that will be mixed together prior to assay, where S is an integer greater than 1; separately subjecting each of said S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence comprising a sample-specific nucleotide tag and a target nucleotide sequence; wherein T is the number of target nucleic acids to be detected, T being an integer greater than one; mixing together tagged target nucleotide sequences from said S samples to form an assay mixture; dividing the assay mixture into up to S × T amplification mixtures, and separately subjecting each of said amplification mixtures to amplification using a unique pair of amplification primers.
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8.发明公开ASSAY METHODS FOR INCREASED THROUGHPUT OF SAMPLES AND/OR TARGETS 审中-公开TESTVERFAHRENFÜRERHÖHTENDURCHSATZ VON PROBEN UND / ODER TARGETS
公开(公告)号：EP2326732A4
公开(公告)日：2012-11-14
申请号：EP09812052
申请日：2009-08-26
Applicant: FLUIDIGM CORP
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/686 , C12N15/1065 , C12Q1/6853 , Y10T436/143333 , C12Q2537/143 , C12Q2525/161
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公开(公告)号：EA020593B1
公开(公告)日：2014-12-30
申请号：EA201170933
申请日：2010-01-13
Applicant: FLUIDIGM CORP
Inventor： HAMILTON AMY , LIN MIN , MIR ALAIN , PIEPRZYK MARTIN
Abstract: Изобретениеотноситсяк способаманализагеномнойДНКи/илиРНКизнебольшихобразцовилидажеединичныхклеток. СпособыанализагеномнойДНКмогутвключатьполногеномнуюамплификацию (WGA) споследующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней. СпособыанализаРНКмогутвключатьобратнуютранскрипциюжелательнойРНКс последующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней.
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公开(公告)号：CA2734868A1
公开(公告)日：2010-03-11
申请号：CA2734868
申请日：2009-08-26
Applicant: FLUIDIGM CORP
Inventor： MIR ALAIN , RAMAKRISHNAN RAMESH , UNGER MARC , ZIMMERMANN BERNHARD G
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
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