公开(公告)号：WO2011040884A3

公开(公告)日：2011-06-03

申请号：PCT/SG2010000369

申请日：2010-09-29

Applicant: FLUIDIGM CORP ,  COHEN DAVID ,  MAY ANDREW ,  PIEPRZYK MARTIN ,  FOWLER BRIAN ,  LEE KIM HUAT ,  YAN JUN ,  ZHOU MING FANG ,  NG SENG BENG

Inventor： COHEN DAVID ,  MAY ANDREW ,  PIEPRZYK MARTIN ,  FOWLER BRIAN ,  LEE KIM HUAT ,  YAN JUN ,  ZHOU MING FANG ,  NG SENG BENG

IPC: G01N35/08 ,  G01N33/48

CPC classification number: B01L3/5027 ,  B01L3/502738 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/12 ,  B01L2300/045 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0655 ,  G01N1/405

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader.

Abstract translation: 本发明包括具有可用可移除覆盖物覆盖的微制造腔体的微流体系统，其中可移除覆盖物允许微制造腔体的至少部分开口被操作员暴露或直接存取。 微流体系统包括腔室，形成在可包含PDMS的弹性体层中的流动和控制通道。 可移除的盖子包括通过粘合层粘合到弹性体层的热塑基膜。 当可移除盖子被剥离时，腔室至少部分打开以允许从腔室抽取样品。 室内可能形成大分子晶体或由PCR反应产生内容物。 本发明还包括一种通过使用可移除盖子来制造弹性体层中的通孔的方法。 本发明进一步包括用于剥离可剥离盖或诸如集成式加热器分散器的可移除部件的方法和装置。

公开(公告)号：SG169918A1

公开(公告)日：2011-04-29

申请号：SG2009066242

申请日：2009-10-02

Applicant: FLUIDIGM CORP

Inventor： COHEN DAVID ,  MAY ANDREW ,  PIEPRZYK MARTIN ,  FOWLER BRIAN ,  LEE KIM HUAT ,  YAN JUN ,  ZHOU MING FANG ,  NG SENG BENG

Abstract: The present invention includes microfluidic systems having a microfabricated cavity that may be covered with a removable cover, where the removable cover allows at least part of the opening of the microfabricated cavity to be exposed or directly accessed by an operator. The microfluidic systems comprise chambers, flow and control channels formed in elastomeric layers that may comprise PDMS. The removable cover comprises a thermoplastic base film bonded to an elastomer layer by an adhesive layer. When the removable cover is peeled off, the chamber is at least partially open to allow sample extraction from the chamber. The chamber may have macromolecular crystals formed inside or resulting contents from a PCR reaction. The invention also includes a method for making vias in elastomeric layers by using the removable cover. The invention further includes methods and devices for peeling the peelable cover or a removable component such as Integrated Heater Spreader. Fig. 1B