公开(公告)号：WO2018013723A1

公开(公告)日：2018-01-18

申请号：PCT/US2017/041770

申请日：2017-07-12

Applicant: FLUIDIGM CORPORATION

Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

IPC: C12Q1/68

Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

Abstract translation: 本文描述了用于制备用于来自细胞群的RNA的单细胞转录物测序的DNA模板的方法。 该方法需要将来自群体的细胞分布到单独的反应体积中，使得多个单独的反应体积各自含有单个分离的细胞，其中细胞在分布之前已经用固定剂处理。 然后使分离的细胞透化或破碎，并通过反转录制备cDNA，然后扩增。 还提供了用于从单细胞中的T细胞受体或免疫球蛋白有效生成DNA模板的新化学物质。

公开(公告)号：WO2018013723A9

公开(公告)日：2018-01-18

申请号：PCT/US2017/041770

申请日：2017-07-12

Applicant: FLUIDIGM CORPORATION

Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

IPC: C12Q1/68

Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

公开(公告)号：EP3485043A1

公开(公告)日：2019-05-22

申请号：EP17828411.3

申请日：2017-07-12

Applicant: Fluidigm Corporation

Inventor： LIVAK, Kenneth J. ,  FEKETE, Richard A.

IPC: C12Q1/68